U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

arp3 ARP2/3 actin-organizing complex actin-related protein subunit Arp3 [ Schizosaccharomyces pombe (fission yeast) ]

Gene ID: 2543233, updated on 10-Jul-2024

Summary

Go to the top of the page Help
Gene symbol
arp3
Gene description
ARP2/3 actin-organizing complex actin-related protein subunit Arp3
Locus tag
SPOM_SPAC630.03
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Schizosaccharomyces pombe (strain: 972h-)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as
act2
Old locus tag
SPAC630.03
NEW
Try the new Gene table
Try the new Transcript table

Genomic context

Go to the top of the page Help
See arp3 in Genome Data Viewer
Location:
chromosome: I
Exon count:
5
Sequence:
Chromosome: I; NC_003424.3 (350433..352296)

Chromosome I - NC_003424.3Genomic Context describing neighboring genes Neighboring gene ncRNA Neighboring gene rRNA-processing protein Neighboring gene GATOR2-SEACAT complex ubiquitin-protein ligase E3 subunit Sea4 Neighboring gene uncharacterized protein Neighboring gene ncRNA Neighboring gene uncharacterized protein Neighboring gene GTPase-activating protein Gyp7

Genomic regions, transcripts, and products

Go to the top of the page Help
Genomic Sequence:
NC_003424.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

Go to the top of the pageHelp

Related articles in PubMed

  1. Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction. Chou SZ, et al. Proc Natl Acad Sci U S A, 2022 Dec 6. PMID 36442092, Free PMC Article
  2. Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state. Shaaban M, et al. Nat Struct Mol Biol, 2020 Nov. PMID 32839613, Free PMC Article
  3. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches. Pandit NG, et al. Proc Natl Acad Sci U S A, 2020 Jun 16. PMID 32461373, Free PMC Article
  4. Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers. Burke TA, et al. Curr Biol, 2014 Mar 3. PMID 24560576, Free PMC Article
  5. Dip1 Co-opts Features of Branching Nucleation to Create Linear Actin Filaments that Activate WASP-Bound Arp2/3 Complex. Balzer CJ, et al. Curr Biol, 2018 Dec 3. PMID 30471998, Free PMC Article

See all (34) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
    Title: Mechanism of actin filament branch formation by Arp2/3 complex revealed by a high-resolution cryo-EM structureof the branch junction.
  2. Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
    Title: Cryo-EM reveals the transition of Arp2/3 complex from inactive to nucleation-competent state.
  3. Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.
    Title: Force and phosphate release from Arp2/3 complex promote dissociation of actin filament branches.
  4. WASp-VCA binding to Arp2/3 protein complex causes a conformational change that favors binding to the side of an actin filament, which allows further changes required to nucleate a daughter filament.
    Title: Conformational changes in Arp2/3 complex induced by ATP, WASp-VCA, and actin filaments.
  5. ARP3 role in the actin filament nucleation.ATP binding destabilizes this interaction via an allosteric link between the Arp3 nucleotide cleft and the hydrophobic groove, thereby promoting the short-pitch conformation.
    Title: Identification of an ATP-controlled allosteric switch that controls actin filament nucleation by Arp2/3 complex.
  6. Arp2/3 complex participates in regulating formin F-actin networks.
    Title: Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers.
  7. Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex
    Title: Insertions within the actin core of actin-related protein 3 (Arp3) modulate branching nucleation by Arp2/3 complex.
  8. Cofilin rapidly dissociates Arp2/3 complex and branches by direct competition for binding sites on the actin filament and by propagation of structural changes in the actin filament that reduce affinity for Arp2/3 complex.
    Title: Cofilin dissociates Arp2/3 complex and branches from actin filaments.
  9. Truncation of the N terminus of ARPC5, the smallest subunit in the complex, increased the yield of Arp2Delta Arp2/3 complex during purification but did not compromise nucleation activity of the full Arp2/3 complex.
    Title: Structure and biochemical properties of fission yeast Arp2/3 complex lacking the Arp2 subunit.
Submit: New GeneRIF Correction

Interactions

Go to the top of the page Help
Products Interactant Other Gene Complex Source Pubs Description

General gene information

Go to the top of the page Help

Homology

Gene Ontology Provided by PomBase

Function Evidence Code Pubs
enables ATP binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables actin filament binding EXP
Inferred from Experiment
more info
 PubMed 
enables actin filament binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in Arp2/3 complex-mediated actin nucleation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in Arp2/3 complex-mediated actin nucleation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in actin cortical patch organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament branching EXP
Inferred from Experiment
more info
 PubMed 
involved_in actin filament polymerization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in endocytosis IC
Inferred by Curator
more info
  
Component Evidence Code Pubs
part_of Arp2/3 protein complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of Arp2/3 protein complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in actin cortical patch IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in actin cortical patch IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cell cortex of cell tip IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cell division site IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm HDA PubMed 
is_active_in cytosol HDA PubMed 

General protein information

Go to the top of the page Help
Preferred Names
ARP2/3 actin-organizing complex actin-related protein subunit Arp3

NCBI Reference Sequences (RefSeq)

Go to the top of the page Help

NEW Try the new Transcript table

Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003424.3 Reference assembly

    Range
    350433..352296
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001018298.3 → NP_592898.1  ARP2/3 actin-organizing complex actin-related protein subunit Arp3 [Schizosaccharomyces pombe]

    See identical proteins and their annotated locations for NP_592898.1

    Status: PROVISIONAL

    UniProtKB/Swiss-Prot
    P32390
    Conserved Domains (1) summary
    PTZ00280
    Location:8 → 425
    PTZ00280; Actin-related protein 3; Provisional
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
3DWL_A.1
3DWL_B.1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

Molecular Biology Databases
Research Materials
Miscellaneous