Mapk8 mitogen-activated protein kinase 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mapk8provided by MGI
Official Full Name: mitogen-activated protein kinase 8provided by MGI
Primary source: MGI:MGI:1346861
See related: Ensembl:ENSMUSG00000021936 AllianceGenome:MGI:1346861
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: JNK; JNK1; Prkm8; SAPK1
Summary: Enables JUN kinase activity and protein serine/threonine/tyrosine kinase activity. Involved in several processes, including generation of neurons; integrin-mediated signaling pathway; and positive regulation of protein metabolic process. Acts upstream of or within several processes, including cellular response to nitric oxide; positive regulation of determination of dorsal identity; and protein phosphorylation. Located in several cellular components, including basal dendrite; mitochondrion; and podosome. Is active in Schaffer collateral - CA1 synapse and cytoplasm. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; sensory organ; and skin. Human ortholog(s) of this gene implicated in abdominal aortic aneurysm; chronic mucocutaneous candidiasis; colorectal adenoma; rectal benign neoplasm; and type 2 diabetes mellitus. Orthologous to human MAPK8 (mitogen-activated protein kinase 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Broad expression in CNS E18 (RPKM 19.1), CNS E14 (RPKM 18.4) and 26 other tissues See more
Orthologs: human all
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Genomic context

Location:: 14 B; 14 20.22 cM

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	14	NC_000080.7 (33099855..33169213, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	14	NC_000080.6 (33377898..33447353, complement)

Chromosome 14 - NC_000080.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

HIF-1alpha is a "brake" in JNK-mediated activation of amyloid protein precursor and hyperphosphorylation of tau induced by T-2 toxin in BV2 cells.
Title: HIF-1α is a "brake" in JNK-mediated activation of amyloid protein precursor and hyperphosphorylation of tau induced by T-2 toxin in BV2 cells.
Knockdown of BUB1B Inhibits the Proliferation, Migration, and Invasion of Colorectal Cancer by Regulating the JNK/c-Jun Signaling Pathway.
Title: Knockdown of BUB1B Inhibits the Proliferation, Migration, and Invasion of Colorectal Cancer by Regulating the JNK/c-Jun Signaling Pathway.
Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI.
Title: Axonal injury mediated by neuronal p75NTR/TRAF6/JNK pathway contributes to cognitive impairment after repetitive mTBI.
Inhibitor of nuclear factor kappa B kinase subunit epsilon regulates murine acetaminophen toxicity via RIPK1/JNK.
Title: Inhibitor of nuclear factor kappa B kinase subunit epsilon regulates murine acetaminophen toxicity via RIPK1/JNK.
Physical exercise elicits UPR[mt] in the skeletal muscle: The role of c-Jun N-terminal kinase.
Title: Physical exercise elicits UPR(mt) in the skeletal muscle: The role of c-Jun N-terminal kinase.
MicroRNA miR-181d-5p regulates the MAPK signaling pathway by targeting mitogen-activated protein kinase 8 (MAPK8) to improve lupus nephritis.
Title: MicroRNA miR-181d-5p regulates the MAPK signaling pathway by targeting mitogen-activated protein kinase 8 (MAPK8) to improve lupus nephritis.
Endoplasmic reticulum chaperone GRP78 participates in fluoride-induced autophagy in LS8 cells by regulating the IRE1-TRAF2-JNK pathway.
Title: Endoplasmic reticulum chaperone GRP78 participates in fluoride-induced autophagy in LS8 cells by regulating the IRE1-TRAF2-JNK pathway.
JNK-JUN-NCOA4 axis contributes to chondrocyte ferroptosis and aggravates osteoarthritis via ferritinophagy.
Title: JNK-JUN-NCOA4 axis contributes to chondrocyte ferroptosis and aggravates osteoarthritis via ferritinophagy.
Transmembrane p24 trafficking protein 10 (TMED10) inhibits mitochondrial damage and protects neurons in ischemic stroke via the c-Jun N-terminal kinase (JNK) signaling pathway.
Title: Transmembrane p24 trafficking protein 10 (TMED10) inhibits mitochondrial damage and protects neurons in ischemic stroke via the c-Jun N-terminal kinase (JNK) signaling pathway.
c-Jun N-terminal kinase 1 (JNK1) phosphorylates OTX2 transcription factor that regulates early retinal development.
Title: c-Jun N-terminal kinase 1 (JNK1) phosphorylates OTX2 transcription factor that regulates early retinal development.

Submit: New GeneRIF Correction See all GeneRIFs (490)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (6)
Gene trapped (1)
Targeted (5) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Mapk8 (e-PCR), detects polymorphism
- UniSTS:527415

STS-L26318 (e-PCR)
- UniSTS:6223

UniSTS:237161 (e-PCR)
- UniSTS:237161

NoName (e-PCR)
- UniSTS:237162

AI849689 (e-PCR)
- UniSTS:180630

RH129367 (e-PCR)
- UniSTS:212674

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables JUN kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables JUN kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables JUN kinase activity	IEA Inferred from Electronic Annotation more info
enables JUN kinase activity	ISO Inferred from Sequence Orthology more info
enables enzyme binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase regulator activity	ISO Inferred from Sequence Orthology more info
enables kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables kinesin binding	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein phosphatase binding	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine/tyrosine kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in JNK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in JNK cascade	IDA Inferred from Direct Assay more info	PubMed
involved_in JNK cascade	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within JNK cascade	ISO Inferred from Sequence Orthology more info	PubMed
involved_in JNK cascade	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within JUN phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within JUN phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in JUN phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in SMAD protein signal transduction	ISO Inferred from Sequence Orthology more info
acts_upstream_of apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to amino acid starvation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to biotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to cadmium ion	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within cellular response to hydrogen peroxide	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within cellular response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info	PubMed
acts_upstream_of_or_within cellular response to nitric oxide	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in cellular response to oxygen-glucose deprivation	ISO Inferred from Sequence Orthology more info
involved_in cellular response to reactive oxygen species	ISO Inferred from Sequence Orthology more info
involved_in dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within determination of dorsal identity	ISO Inferred from Sequence Orthology more info	PubMed
involved_in energy homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in integrin-mediated signaling pathway	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IDA Inferred from Direct Assay more info	PubMed
involved_in modulation of chemical synaptic transmission	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of protein binding	ISO Inferred from Sequence Orthology more info
involved_in neuron migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron projection development	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within neuronal stem cell population maintenance	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within ossification	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within peptidyl-serine phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-serine phosphorylation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within peptidyl-threonine phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-threonine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA replication	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of NLRP3 inflammasome complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of NLRP3 inflammasome complex assembly	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within positive regulation of apoptotic signaling pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of cardiac muscle cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cyclase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of determination of dorsal identity	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of gene expression	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of glial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of microtubule polymerization	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuroblast proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of neuron migration	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of podosome assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein metabolic process	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of protein ubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within programmed necrotic cell death	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to tricellular tight junction	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in regulation of centrosome cycle	ISO Inferred from Sequence Orthology more info
involved_in regulation of circadian rhythm	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of dense core granule transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within regulation of gene expression	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in regulation of protein localization	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to UV	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within response to cadmium ion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to hydrogen peroxide	ISO Inferred from Sequence Orthology more info
involved_in response to mechanical stimulus	IBA Inferred from Biological aspect of Ancestor more info
involved_in response to oxidative stress	ISO Inferred from Sequence Orthology more info
involved_in rhythmic process	IEA Inferred from Electronic Annotation more info
involved_in stress-activated MAPK cascade	ISO Inferred from Sequence Orthology more info
involved_in type B pancreatic cell apoptotic process	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in Schaffer collateral - CA1 synapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in Schaffer collateral - CA1 synapse	IMP Inferred from Mutant Phenotype more info	PubMed
located_in axon	IMP Inferred from Mutant Phenotype more info	PubMed
located_in axon	ISO Inferred from Sequence Orthology more info
located_in basal dendrite	IMP Inferred from Mutant Phenotype more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	IMP Inferred from Mutant Phenotype more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite cytoplasm	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in neuron projection	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in perikaryon	ISO Inferred from Sequence Orthology more info
located_in podosome	IDA Inferred from Direct Assay more info	PubMed
located_in synapse	ISO Inferred from Sequence Orthology more info
located_in synapse	ISS Inferred from Sequence or Structural Similarity more info
located_in vesicle	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: mitogen-activated protein kinase 8

Names: JNK1 beta1 protein kinase; MAP kinase 8; MAPK 8; c-Jun N-terminal kinase 1; protein kinase mitogen-activated 8; stress-activated protein kinase JNK1

NP_001297381.1

EC 2.7.11.24

NP_001297382.1

EC 2.7.11.24

NP_001297383.1

EC 2.7.11.24

NP_057909.1

EC 2.7.11.24

XP_030103680.1

EC 2.7.11.24

XP_036014495.1

EC 2.7.11.24

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001310452.2 → NP_001297381.1 mitogen-activated protein kinase 8 isoform 2

See identical proteins and their annotated locations for NP_001297381.1

Status: VALIDATED

Description

Transcript Variant: This variant (2) differs in its 5' UTR and both contains and lacks an alternate in-frame exon in the central coding region compared to variant 1. It encodes isoform 2 which is the same length as isoform 1, but differs in a small segment in the central region of the protein, compared to isoform 1.

Source sequence(s)

AC154649

Consensus CDS

CCDS84111.1

UniProtKB/Swiss-Prot

Q61831, Q9R0U6

UniProtKB/TrEMBL

A6P3E4

Related

ENSMUSP00000107574.2, ENSMUST00000111943.8

Conserved Domains (1) summary

cd07850
Location:25 → 360

STKc_JNK; Catalytic domain of the Serine/Threonine Kinase, c-Jun N-terminal Kinase
NM_001310453.2 → NP_001297382.1 mitogen-activated protein kinase 8 isoform 4

See identical proteins and their annotated locations for NP_001297382.1

Status: VALIDATED

Description

Transcript Variant: This variant (4) both contains and lacks an alternate in-frame exon in the central coding region, and uses an alternate splice donor site in its 3' terminal exon which results in a frameshift, compared to variant 1. It encodes isoform 4 has an alternate region in the central region of the protein and a longer and distinct C-terminus, compared to isoform 1. Isoforms 3 and 4 are the same length, but differ in a small segment in the central region of the protein.

Source sequence(s)

AC154649

Consensus CDS

CCDS79293.1

UniProtKB/TrEMBL

G3X8U9

Related

ENSMUSP00000022504.6, ENSMUST00000022504.12

Conserved Domains (1) summary

cd07850
Location:25 → 360

STKc_JNK; Catalytic domain of the Serine/Threonine Kinase, c-Jun N-terminal Kinase
NM_001310454.2 → NP_001297383.1 mitogen-activated protein kinase 8 isoform 3

See identical proteins and their annotated locations for NP_001297383.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) uses an alternate splice donor site in its 3' terminal exon which results in a frameshift, compared to variant 1. It encodes isoform 3, which has a longer and distinct C-terminus, compared to isoform 1. Isoforms 3 and 4 are the same length, but differ in a small segment in the central region of the protein.

Source sequence(s)

AC154649

Consensus CDS

CCDS79295.1

UniProtKB/TrEMBL

Q7TSJ7

Related

ENSMUSP00000107575.4, ENSMUST00000111944.10

Conserved Domains (1) summary

cd07850
Location:25 → 360

STKc_JNK; Catalytic domain of the Serine/Threonine Kinase, c-Jun N-terminal Kinase
NM_016700.5 → NP_057909.1 mitogen-activated protein kinase 8 isoform 1

See identical proteins and their annotated locations for NP_057909.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript. Isoforms 1 and 2 are the same length, but differ in a small segment in the central region of the protein.

Source sequence(s)

AC154649

Consensus CDS

CCDS36869.1

UniProtKB/Swiss-Prot

Q61831, Q91Y86, Q9R0U6

UniProtKB/TrEMBL

Q544A0

Related

ENSMUSP00000107576.3, ENSMUST00000111945.9

Conserved Domains (1) summary

cd07850
Location:25 → 360

STKc_JNK; Catalytic domain of the Serine/Threonine Kinase, c-Jun N-terminal Kinase

RNA

NR_188805.1 RNA Sequence

Status: VALIDATED

Source sequence(s)

AC154649

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000080.7 Reference GRCm39 C57BL/6J

Range

33099855..33169213 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036158602.1 → XP_036014495.1 mitogen-activated protein kinase 8 isoform X4

Conserved Domains (1) summary

cl21453
Location:1 → 180

PKc_like; Protein Kinases, catalytic domain
XM_030247820.2 → XP_030103680.1 mitogen-activated protein kinase 8 isoform X2

Conserved Domains (1) summary

cl21453
Location:1 → 180

PKc_like; Protein Kinases, catalytic domain