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Orc4 origin recognition complex, subunit 4 [ Mus musculus (house mouse) ]

Gene ID: 26428, updated on 2-Nov-2024

Summary

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Official Symbol
Orc4provided by MGI
Official Full Name
origin recognition complex, subunit 4provided by MGI
Primary source
MGI:MGI:1347043
See related
Ensembl:ENSMUSG00000026761 AllianceGenome:MGI:1347043
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Orc4P; Orc4l; mMmORC4
Summary
Predicted to enable DNA replication origin binding activity and nucleotide binding activity. Involved in polar body extrusion after meiotic divisions and protein polymerization. Acts upstream of or within DNA replication. Is active in cytoplasm. Is expressed in brain; embryo; and pancreas epithelium. Human ortholog(s) of this gene implicated in Meier-Gorlin syndrome 2. Orthologous to human ORC4 (origin recognition complex subunit 4). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Broad expression in CNS E18 (RPKM 9.0), CNS E14 (RPKM 7.9) and 25 other tissues See more
Orthologs
human all
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Genomic context

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See Orc4 in Genome Data Viewer
Location:
2 C1.1; 2 28.42 cM
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 2 NC_000068.8 (48792836..48840287, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 2 NC_000068.7 (48902824..48952902, complement)

Chromosome 2 - NC_000068.8Genomic Context describing neighboring genes Neighboring gene predicted gene, 31324 Neighboring gene glyceraldehyde-3-phosphate dehydrogenase pseudogene Neighboring gene activin receptor IIA Neighboring gene CapStarr-seq enhancer MGSCv37_chr2:48804892-48805001 Neighboring gene methyl-CpG binding domain protein 5 Neighboring gene predicted gene 13510 Neighboring gene adaptor-related protein complex 2, mu 1 subunit, pseudogene

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion. Nguyen H, et al. Syst Biol Reprod Med, 2020 Dec. PMID 32972244, Free PMC Article
  2. Higher Order Oligomerization of the Licensing ORC4 Protein Is Required for Polar Body Extrusion in Murine Meiosis. Nguyen H, et al. J Cell Biochem, 2017 Sep. PMID 28230328, Free PMC Article
  3. ORC4 surrounds extruded chromatin in female meiosis. Nguyen H, et al. J Cell Biochem, 2015 May. PMID 25502171, Free PMC Article
  4. Deletion of Orc4 during oogenesis severely reduces polar body extrusion and blocks zygotic DNA replication†. Nguyen H, et al. Biol Reprod, 2022 Apr 26. PMID 34977916, Free PMC Article
  5. Identification and characterization of MmORC4 and MmORC5, two subunits of the mouse origin of replication recognition complex. Springer J, et al. Chromosoma, 1999 Aug. PMID 10460412

See all (28) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
    Title: The role of ORC4 in enucleation of Murine Erythroleukemia (MEL) cells is similar to that in oocyte polar body extrusion.
  2. Together, these data demonstrate that ORC4 oligomerization is required to form the ORC4 cage and that it is required for polar body extrusion.
    Title: Higher Order Oligomerization of the Licensing ORC4 Protein Is Required for Polar Body Extrusion in Murine Meiosis.
  3. ORC4 surrounds the chromatin that will be extruded in the polar body in both female meiotic divisions, then makes a transition from the cytoplasm to the chromosomes at zygotic anaphase, suggesting multiple roles for this replication licensing protein.
    Title: ORC4 surrounds extruded chromatin in female meiosis.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (1) 
  • Gene trapped (1) 
  • Targeted (3)  1 citation

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA replication origin binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA replication origin binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA replication origin binding ISO
Inferred from Sequence Orthology
more info
  
enables nucleotide binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within DNA replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within DNA replication TAS
Traceable Author Statement
more info
 PubMed 
involved_in DNA replication initiation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA replication initiation ISO
Inferred from Sequence Orthology
more info
  
involved_in polar body extrusion after meiotic divisions IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein polymerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
located_in chromosome, telomeric region IEA
Inferred from Electronic Annotation
more info
  
colocalizes_with chromosome, telomeric region ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
part_of nuclear origin of replication recognition complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of nuclear origin of replication recognition complex ISO
Inferred from Sequence Orthology
more info
  
part_of nuclear origin of replication recognition complex ISS
Inferred from Sequence or Structural Similarity
more info
  
part_of nuclear origin of replication recognition complex NAS
Non-traceable Author Statement
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleolus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
part_of origin recognition complex ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
origin recognition complex subunit 4

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001177313.2 → NP_001170784.1  origin recognition complex subunit 4 isoform 2

    See identical proteins and their annotated locations for NP_001170784.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) lacks multiple 3' exons, but has an alternate 3' exon, as compared to variant 1. The resulting isoform (2) is shorter and has a different C-terminus, as compared to isoform 1. Variants 2, 11, 12, and 13 all encode the same isoform (2).
    Source sequence(s)
    AL732317, AL845521
    Consensus CDS
    CCDS50582.1
    UniProtKB/TrEMBL
    Q9CZF7
    Related
    ENSMUSP00000088497.4, ENSMUST00000090976.10
    Conserved Domains (2) summary
    PRK00411
    Location:46 → 130
    cdc6; ORC1-type DNA replication protein
    pfam13191
    Location:35 → 82
    AAA_16; AAA ATPase domain

  2. NM_001355296.2 → NP_001342225.1  origin recognition complex subunit 4 isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 4, 7, and 8 all encode the same isoform (1).
    Source sequence(s)
    AL732317, AL845521
    Consensus CDS
    CCDS16022.1
    UniProtKB/Swiss-Prot
    O88708, Q91V62, Q9QYX1
    UniProtKB/TrEMBL
    Q8BN98, Q8C693
    Conserved Domains (2) summary
    COG1474
    Location:41 → 223
    CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
    pfam14629
    Location:225 → 412
    ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus

  3. NM_001421264.1 → NP_001408193.1  origin recognition complex subunit 4 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    UniProtKB/Swiss-Prot
    O88708, Q91V62, Q9QYX1

  4. NM_001421265.1 → NP_001408194.1  origin recognition complex subunit 4 isoform 1

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    UniProtKB/Swiss-Prot
    O88708, Q91V62, Q9QYX1

  5. NM_001421266.1 → NP_001408195.1  origin recognition complex subunit 4 isoform 3

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521

  6. NM_001421267.1 → NP_001408196.1  origin recognition complex subunit 4 isoform 4

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    Related
    ENSMUSP00000119274.2, ENSMUST00000142851.8

  7. NM_001421268.1 → NP_001408197.1  origin recognition complex subunit 4 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    UniProtKB/TrEMBL
    Q9CZF7

  8. NM_001421269.1 → NP_001408198.1  origin recognition complex subunit 4 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    UniProtKB/TrEMBL
    Q9CZF7

  9. NM_001421270.1 → NP_001408199.1  origin recognition complex subunit 4 isoform 2

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    UniProtKB/TrEMBL
    Q9CZF7

  10. NM_011958.5 → NP_036088.3  origin recognition complex subunit 4 isoform 1

    See identical proteins and their annotated locations for NP_036088.3

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1, 4, 7, and 8 all encode the same isoform (1).
    Source sequence(s)
    AL732317, AL845521
    Consensus CDS
    CCDS16022.1
    UniProtKB/Swiss-Prot
    O88708, Q91V62, Q9QYX1
    UniProtKB/TrEMBL
    Q8BN98, Q8C693
    Related
    ENSMUSP00000028098.5, ENSMUST00000028098.11
    Conserved Domains (2) summary
    COG1474
    Location:41 → 223
    CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
    pfam14629
    Location:225 → 412
    ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus

RNA

  1. NR_033442.3 RNA Sequence

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) lacks an internal segment in the 3' CDS, as compared to variant 1. The transcript is a nonsense-mediated mRNA decay candidate and is probably not making a protein.
    Source sequence(s)
    AL732317, AL845521

  2. NR_149355.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521

  3. NR_149356.2 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521

  4. NR_185286.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521

  5. NR_185287.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521
    Related
    ENSMUST00000154784.8

  6. NR_185288.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521

  7. NR_185289.1 RNA Sequence

    Status: VALIDATED

    Source sequence(s)
    AL732317, AL845521

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000068.8 Reference GRCm39 C57BL/6J

    Range
    48792836..48840287 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_006498066.4 → XP_006498129.1  origin recognition complex subunit 4 isoform X2

    UniProtKB/TrEMBL
    Q8BN98, Q8C693
    Conserved Domains (2) summary
    COG1474
    Location:41 → 190
    CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
    pfam14629
    Location:195 → 354
    ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus

  2. XM_030251581.1 → XP_030107441.1  origin recognition complex subunit 4 isoform X2

    UniProtKB/TrEMBL
    Q8BN98, Q8C693
    Conserved Domains (2) summary
    COG1474
    Location:41 → 190
    CDC6; Cdc6-related protein, AAA superfamily ATPase [Replication, recombination and repair]
    pfam14629
    Location:195 → 354
    ORC4_C; Origin recognition complex (ORC) subunit 4 C-terminus
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O88708.2 GenPept UniProtKB/Swiss-Prot:O88708

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