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Nit2 nitrilase family, member 2 [ Rattus norvegicus (Norway rat) ]

Gene ID: 288174, updated on 2-Nov-2024

Summary

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Official Symbol
Nit2provided by RGD
Official Full Name
nitrilase family, member 2provided by RGD
Primary source
RGD:1310494
See related
EnsemblRapid:ENSRNOG00000027797 AllianceGenome:RGD:1310494
Gene type
protein coding
RefSeq status
PROVISIONAL
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
RGD1310494
Summary
Predicted to enable omega-amidase activity. Predicted to be involved in asparagine metabolic process; glutamine metabolic process; and oxaloacetate metabolic process. Predicted to be located in centrosome; cytosol; and mitochondrion. Orthologous to human NIT2 (nitrilase family member 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in Liver (RPKM 1128.0), Kidney (RPKM 1005.5) and 8 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Nit2 in Genome Data Viewer
Location:
11q12
Exon count:
10
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 11 NC_086029.1 (56832957..56844142)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 11 NC_051346.1 (43363839..43378713)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 11 NC_005110.4 (45462345..45473107)

Chromosome 11 - NC_086029.1Genomic Context describing neighboring genes Neighboring gene TBC1 domain family, member 23 Neighboring gene transfer RNA alanine (anticodon AGC) 25 Neighboring gene ribosome biogenesis protein NSA2 homolog pseudogene Neighboring gene translocase of outer mitochondrial membrane 70 Neighboring gene uncharacterized LOC108352328

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination. Krasnikov BF, et al. Biochimie, 2009 Sep. PMID 19595734, Free PMC Article
  2. Structural insights into the catalytic active site and activity of human Nit2/ω-amidase: kinetic assay and molecular dynamics simulation. Chien CH, et al. J Biol Chem, 2012 Jul 27. PMID 22674578, Free PMC Article
  3. Interacting Network of the Gap Junction (GJ) Protein Connexin43 (Cx43) is Modulated by Ischemia and Reperfusion in the Heart. Martins-Marques T, et al. Mol Cell Proteomics, 2015 Nov. PMID 26316108, Free PMC Article
  4. Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Mootha VK, et al. Cell, 2003 Nov 26. PMID 14651853
  5. Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context. Morgenstern M, et al. Cell Metab, 2021 Dec 7. PMID 34800366, Free PMC Article

See all (9) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Data report that rat liver Nit2 is omega-amidase and in vivo amidase substrates are alpha-ketoglutaramate and alpha-ketosuccinamate, generated by transamination of glutamine and asparagine, respectively.
    Title: Identification of the putative tumor suppressor Nit2 as omega-amidase, an enzyme metabolically linked to glutamine and asparagine transamination.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC124762

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables 2-oxoglutaramate amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides IEA
Inferred from Electronic Annotation
more info
  
enables omega-amidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables omega-amidase activity IEA
Inferred from Electronic Annotation
more info
  
enables omega-amidase activity ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in asparagine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in asparagine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in asparagine metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in glutamine metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in glutamine metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in glutamine metabolic process ISO
Inferred from Sequence Orthology
more info
  
involved_in oxaloacetate metabolic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in oxaloacetate metabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in oxaloacetate metabolic process ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in centrosome IEA
Inferred from Electronic Annotation
more info
  
located_in centrosome ISO
Inferred from Sequence Orthology
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
 PubMed 

General protein information

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Preferred Names
omega-amidase NIT2
Names
Nit protein 2
nitrilase homolog 2
NP_001029298.1
XP_038944121.1
XP_038944122.1
XP_063126479.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001034126.1 → NP_001029298.1  omega-amidase NIT2

    See identical proteins and their annotated locations for NP_001029298.1

    Status: PROVISIONAL

    Source sequence(s)
    BC100637
    UniProtKB/Swiss-Prot
    Q497B0
    UniProtKB/TrEMBL
    A0A8I5ZM02, A6IQN1
    Related
    ENSRNOP00000078811.1, ENSRNOT00000105850.2
    Conserved Domains (1) summary
    cd07572
    Location:5 → 265
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086029.1 Reference GRCr8

    Range
    56832957..56844142
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_039088193.1 → XP_038944121.1  omega-amidase NIT2 isoform X1

    UniProtKB/TrEMBL
    A0A8L2QJE6
    Related
    ENSRNOP00000034144.5, ENSRNOT00000029420.6
    Conserved Domains (1) summary
    cd07572
    Location:89 → 349
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

  2. XM_039088194.2 → XP_038944122.1  omega-amidase NIT2 isoform X2

    UniProtKB/TrEMBL
    A0A8L2QJE6
    Conserved Domains (1) summary
    cd07572
    Location:89 → 316
    nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)

  3. XM_063270409.1 → XP_063126479.1  omega-amidase NIT2 isoform X3

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q497B0.1 GenPept UniProtKB/Swiss-Prot:Q497B0

Gene LinkOut

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