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Atp1a4 ATPase Na+/K+ transporting subunit alpha 4 [ Rattus norvegicus (Norway rat) ]

Gene ID: 29132, updated on 10-Oct-2024

Summary

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Official Symbol
Atp1a4provided by RGD
Official Full Name
ATPase Na+/K+ transporting subunit alpha 4provided by RGD
Primary source
RGD:61952
See related
EnsemblRapid:ENSRNOG00000032378 AllianceGenome:RGD:61952
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary
Enables P-type sodium:potassium-exchanging transporter activity. Involved in flagellated sperm motility; regulation of cellular pH; and spermatogenesis. Predicted to be located in rod photoreceptor outer segment and sperm midpiece. Predicted to be part of sodium:potassium-exchanging ATPase complex. Orthologous to human ATP1A4 (ATPase Na+/K+ transporting subunit alpha 4). [provided by Alliance of Genome Resources, Apr 2022]
Expression
Biased expression in Testes (RPKM 585.2), Muscle (RPKM 104.5) and 1 other tissue See more
Orthologs
human mouse all
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Genomic context

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See Atp1a4 in Genome Data Viewer
Location:
13q24
Exon count:
23
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) 13 NC_086031.1 (87216139..87252155, complement)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) 13 NC_051348.1 (84683766..84719790, complement)
106 previous assembly Rnor_6.0 (GCF_000001895.5) 13 NC_005112.4 (90605792..90642032, complement)

Chromosome 13 - NC_086031.1Genomic Context describing neighboring genes Neighboring gene DDB1 and CUL4 associated factor 8 Neighboring gene proliferation and apoptosis adaptor protein 15 Neighboring gene calsequestrin 1 Neighboring gene ATPase Na+/K+ transporting subunit alpha 2 Neighboring gene immunoglobulin superfamily, member 8 Neighboring gene uncharacterized LOC134481592 Neighboring gene potassium inwardly-rectifying channel, subfamily J, member 9

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. The α4 isoform of the Na⁺,K⁺-ATPase is tuned for changing extracellular environments. Clausen MV, et al. FEBS J, 2016 Jan. PMID 26476261
  2. Activity of the Na,K-ATPase α4 isoform is regulated during sperm capacitation to support sperm motility. Jimenez T, et al. J Androl, 2012 Sep-Oct. PMID 22441762
  3. Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa. Jimenez T, et al. Reproduction, 2010 May. PMID 20179187
  4. Different expression and activity of the alpha1 and alpha4 isoforms of the Na,K-ATPase during rat male germ cell ontogeny. Wagoner K, et al. Reproduction, 2005 Nov. PMID 16264093
  5. Functional expression of the alpha4 isoform of the Na,K-ATPase in both diploid and haploid germ cells of male rats. Blanco G. Ann N Y Acad Sci, 2003 Apr. PMID 12763881

See all (17) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Ouabain interactions with the alpha4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatocytes.
    Title: Ouabain interactions with the α4 isoform of the sodium pump trigger non-classical steroid hormone signaling and integrin expression in spermatogenic cells.
  2. Atp1a4 is tuned for changing extracellular environments during sperm capacitation.
    Title: The α4 isoform of the Na⁺,K⁺-ATPase is tuned for changing extracellular environments.
  3. Na,K-ATPase alpha4 activity is up-regulated during sperm capacitation through mechanisms that involve both increases in molecular activity and levels of alpha4 at the sperm plasma membrane.
    Title: Activity of the Na,K-ATPase α4 isoform is regulated during sperm capacitation to support sperm motility.
  4. These results provide new genetic evidence for the role of the ATP1A4 isoform in sperm motility, under both noncapacitating and capacitating conditions.
    Title: Increased expression of the Na,K-ATPase alpha4 isoform enhances sperm motility in transgenic mice.
  5. In sperm, alpha4 is isoform primarily involved in controlling transmembrane Na+ gradient; alpha4 activity is necessary for maintaining membrane potential, [Ca2+]i, and [H+]i. Thus, alpha4 activity is necessary to maintain sperm motility.
    Title: Activity of the Na,K-ATPase alpha4 isoform is important for membrane potential, intracellular Ca2+, and pH to maintain motility in rat spermatozoa.
  6. This isoform is developmentally expressed in haploid and diploid spermatozoa.
    Title: Functional expression of the alpha4 isoform of the Na,K-ATPase in both diploid and haploid germ cells of male rats.
Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Clone Names

  • MGC93426

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATPase-coupled monoatomic cation transmembrane transporter activity ISO
Inferred from Sequence Orthology
more info
  
enables P-type sodium:potassium-exchanging transporter activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables P-type sodium:potassium-exchanging transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables P-type sodium:potassium-exchanging transporter activity IEA
Inferred from Electronic Annotation
more info
  
enables P-type sodium:potassium-exchanging transporter activity ISO
Inferred from Sequence Orthology
more info
  
enables P-type sodium:potassium-exchanging transporter activity TAS
Traceable Author Statement
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in establishment of localization in cell IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within establishment of localization in cell ISO
Inferred from Sequence Orthology
more info
  
involved_in establishment or maintenance of transmembrane electrochemical gradient IEA
Inferred from Electronic Annotation
more info
  
involved_in fertilization IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within fertilization ISO
Inferred from Sequence Orthology
more info
  
involved_in flagellated sperm motility IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in flagellated sperm motility IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within flagellated sperm motility ISO
Inferred from Sequence Orthology
more info
  
involved_in flagellated sperm motility ISO
Inferred from Sequence Orthology
more info
  
involved_in flagellated sperm motility ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in intracellular potassium ion homeostasis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in intracellular sodium ion homeostasis IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in potassium ion import across plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in potassium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in potassium ion transmembrane transport ISO
Inferred from Sequence Orthology
more info
  
involved_in potassium ion transport TAS
Traceable Author Statement
more info
 PubMed 
involved_in proton transmembrane transport IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of cellular pH IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in regulation of cellular pH IEA
Inferred from Electronic Annotation
more info
  
involved_in regulation of cellular pH ISO
Inferred from Sequence Orthology
more info
  
involved_in regulation of cellular pH ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in regulation of membrane potential IEA
Inferred from Electronic Annotation
more info
  
acts_upstream_of_or_within regulation of membrane potential ISO
Inferred from Sequence Orthology
more info
  
involved_in sodium ion export across plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in sodium ion transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
involved_in sodium ion transmembrane transport ISO
Inferred from Sequence Orthology
more info
  
acts_upstream_of_or_within sodium ion transport ISO
Inferred from Sequence Orthology
more info
  
involved_in sodium ion transport TAS
Traceable Author Statement
more info
 PubMed 
involved_in spermatogenesis IEA
Inferred from Electronic Annotation
more info
  
involved_in spermatogenesis IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within spermatogenesis ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
is_active_in cell projection IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in photoreceptor cell cilium ISO
Inferred from Sequence Orthology
more info
  
is_active_in plasma membrane IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in rod photoreceptor outer segment IEA
Inferred from Electronic Annotation
more info
  
located_in rod photoreceptor outer segment ISO
Inferred from Sequence Orthology
more info
  
part_of sodium:potassium-exchanging ATPase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of sodium:potassium-exchanging ATPase complex IEA
Inferred from Electronic Annotation
more info
  
part_of sodium:potassium-exchanging ATPase complex ISO
Inferred from Sequence Orthology
more info
  
part_of sodium:potassium-exchanging ATPase complex TAS
Traceable Author Statement
more info
 PubMed 
located_in sperm midpiece IEA
Inferred from Electronic Annotation
more info
  
located_in sperm midpiece ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
sodium/potassium-transporting ATPase subunit alpha-4
Names
ATPase, Na+/K+ transporting, alpha 4 polypeptide
Na(+)/K(+) ATPase alpha-4 subunit
sodium pump subunit alpha-4
sodium/potassium-transporting ATPase subunit alpha-4-like
NP_001257959.1
NP_074039.1
XP_017454251.1
XP_063128272.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001271030.1 → NP_001257959.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 1

    See identical proteins and their annotated locations for NP_001257959.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.
    Source sequence(s)
    BC087015, JAXUCZ010000013
    UniProtKB/Swiss-Prot
    Q64541
    UniProtKB/TrEMBL
    Q5PQV3
    Conserved Domains (1) summary
    cd02608
    Location:67 → 1025
    P-type_ATPase_Na-K_like; alpha-subunit of Na(+)/K(+)-ATPases and of gastric H(+)/K(+)-ATPase, similar to the human Na(+)/K(+)-ATPase alpha subunits 1-4

  2. NM_001429529.1 → NP_001416458.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 2

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

  3. NM_001429530.1 → NP_001416459.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 3

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

  4. NM_001429533.1 → NP_001416462.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 4

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

  5. NM_001429534.1 → NP_001416463.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 4

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

  6. NM_001429536.1 → NP_001416465.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 5

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

  7. NM_001429537.1 → NP_001416466.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 6

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000013

  8. NM_022848.3 → NP_074039.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform 1

    See identical proteins and their annotated locations for NP_074039.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) is the longer transcript. Both variants 1 and 2 encode the same protein.
    Source sequence(s)
    JAXUCZ010000013
    UniProtKB/Swiss-Prot
    Q64541
    UniProtKB/TrEMBL
    Q5PQV3
    Related
    ENSRNOP00000062079.3, ENSRNOT00000064601.5
    Conserved Domains (1) summary
    cd02608
    Location:67 → 1025
    P-type_ATPase_Na-K_like; alpha-subunit of Na(+)/K(+)-ATPases and of gastric H(+)/K(+)-ATPase, similar to the human Na(+)/K(+)-ATPase alpha subunits 1-4

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086031.1 Reference GRCr8

    Range
    87216139..87252155 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_017598762.3 → XP_017454251.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform X1

  2. XM_063272202.1 → XP_063128272.1  sodium/potassium-transporting ATPase subunit alpha-4 isoform X1

Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q64541.1 GenPept UniProtKB/Swiss-Prot:Q64541

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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