AGO3 Argonaute 3 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: AGO3provided by FlyBase
Official Full Name: Argonaute 3provided by FlyBase
Primary source: FLYBASE:FBgn0250816
Locus tag: Dmel_CG40300
See related: AllianceGenome:FB:FBgn0250816
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: Ago-3; AGO-3; ago3; Ago3; BEST:LD15785; CG40300; dAgo3; Dmel\CG40300
Summary: Enables piRNA binding activity. Involved in negative regulation of transposition; positive regulation of nuclear-transcribed mRNA poly(A) tail shortening; and post-transcriptional gene silencing by RNA. Located in P granule; cytosol; and mitochondrion. Is expressed in germarium; male germline stem cell; primary gonial cell; spermatogonium; and spermatozoon. Orthologous to several human genes including PIWIL1 (piwi like RNA-mediated gene silencing 1) and PIWIL2 (piwi like RNA-mediated gene silencing 2). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 80F9-80F9; 3-47 cM

Exon count:: 9

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (23554593..23689640)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (23547693..23682740)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We propose that Armi shuttles between nuage and mitochondria, feeding precursor piRNAs generated by Ago3 cleavage into the Zucchini-dependent production of Aubergine- and Piwi-bound piRNAs on the mitochondrial surface.
Title: The RNA-Binding ATPase, Armitage, Couples piRNA Amplification in Nuage to Phased piRNA Production on Mitochondria.
Data show that Cbp80 knockdown not only led to the upregulation of transposons, but also to delocalization of Piwi, Aub and Ago3, key factors in the piRNA biosynthesis pathway.
Title: Cbp80 is needed for the expression of piRNA components and piRNAs.
The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules.
Title: RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.
We find there are large differences in evolutionary rates and gene turnover between pathways, and that paralogs of Ago2, Ago3, and Piwi/Aub show contrasting rates of evolution after duplication.
Title: Duplication and Diversification of Dipteran Argonaute Genes, and the Evolutionary Divergence of Piwi and Aubergine.
Krimper interacts directly with Aub and Ago3 to coordinate the assembly of the ping-pong piRNA processing complex.
Title: Aub and Ago3 Are Recruited to Nuage through Two Mechanisms to Form a Ping-Pong Complex Assembled by Krimper.
Krimp enforces an antisense bias on piRNA pools by binding AGO3 and blocking its access to primary piRNAs.
Title: Krimper Enforces an Antisense Bias on piRNA Pools by Binding AGO3 in the Drosophila Germline.
Although Argonaute3 slicing is required to efficiently trigger phased piRNA production, an alternative, slicing-independent pathway suffices to generate Piwi-bound piRNAs that repress transcription of a subset of transposon families.
Title: Slicing and Binding by Ago3 or Aub Trigger Piwi-Bound piRNA Production by Distinct Mechanisms.
AGO3 Slicer activity is essential for piRNA amplification.
Title: AGO3 Slicer activity regulates mitochondria-nuage localization of Armitage and piRNA amplification.
PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition.
Title: PAPI, a novel TUDOR-domain protein, complexes with AGO3, ME31B and TRAL in the nuage to silence transposition.
Biogenesis pathways of piRNAs loaded onto AGO3 in testes.
Title: Biogenesis pathways of piRNAs loaded onto AGO3 in the Drosophila testis.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: 23556291-23564561 mobile_element_type = transposon:jockey{}4145
Loc: 23557459-23559016 mobile_element_type = transposon:Tc1-2{}1779
Loc: 23561817-23563262 mobile_element_type = transposon:gypsy9{}4146
Loc: 23564562-23564897 mobile_element_type = transposon:INE-1{}5799
Loc: 23566234-23566378 mobile_element_type = transposon:Cr1a{}5909
Loc: 23566455-23566513 mobile_element_type = transposon:INE-1{}5805
Loc: 23567922-23568360 mobile_element_type = transposon:INE-1{}5806
Loc: 23578718-23579309 mobile_element_type = transposon:Idefix{}1817
Loc: 23579845-23580227 mobile_element_type = transposon:INE-1{}5808
Loc: 23587272-23588633 mobile_element_type = transposon:Stalker2{}4148
Loc: 23587600-23588378 mobile_element_type = transposon:412{}1862
Loc: 23588763-23588827 mobile_element_type = transposon:Doc4{}5900
Loc: 23588945-23589011 mobile_element_type = transposon:Doc{}5864
Loc: 23589012-23589090 mobile_element_type = transposon:INE-1{}5814
Loc: 23589469-23589847 mobile_element_type = transposon:1360{}5894
Loc: 23591540-23592122 mobile_element_type = transposon:1360{}4149
Loc: 23599889-23600309 mobile_element_type = transposon:roo{}5834
Loc: 23608556-23609267 mobile_element_type = transposon:G5{}1777
Loc: 23609268-23610746 mobile_element_type = transposon:G5A{}5879
Loc: 23611484-23612236 mobile_element_type = transposon:baggins{}1812
Loc: 23612488-23612987 mobile_element_type = transposon:McClintock{}5830
Loc: 23613961-23614216 mobile_element_type = transposon:INE-1{}5815
Loc: 23617025-23617237 mobile_element_type = transposon:INE-1{}5816
Loc: 23617767-23618959 mobile_element_type = transposon:INE-1{}5819
Loc: 23620654-23625375 mobile_element_type = transposon:Doc{}2375
Loc: 23630623-23631881 mobile_element_type = transposon:BS3{}4609
Loc: 23632603-23632677 mobile_element_type = transposon:accord{}2988
Loc: 23637661-23639375 mobile_element_type = transposon:Doc3{}2946
Loc: 23639714-23640401 mobile_element_type = transposon:diver2{}2939
Loc: 23645192-23645726 mobile_element_type = transposon:gypsy8{}2955
Loc: 23648443-23648701 mobile_element_type = transposon:INE-1{}1889
Loc: 23650387-23651476 mobile_element_type = transposon:1360{}2399
Loc: 23653666-23654025 mobile_element_type = transposon:INE-1{}1891
Loc: 23654285-23655248 mobile_element_type = transposon:Tc1-2{}4610
Loc: 23655493-23655916 mobile_element_type = transposon:roo{}2355
Loc: 23656426-23657062 mobile_element_type = transposon:G4{}2381
Loc: 23657339-23661870 mobile_element_type = transposon:gypsy2{}4611
Loc: 23661896-23662342 mobile_element_type = transposon:Porto1{}2979
Loc: 23665328-23665420 mobile_element_type = transposon:INE-1{}1893
Loc: 23668448-23668765 mobile_element_type = transposon:GATE{}2925
Loc: 23681533-23681641 mobile_element_type = transposon:F{}4612
Loc: 23682825-23682910 mobile_element_type = transposon:INE-1{}1894
Loc: 23682913-23683224 mobile_element_type = transposon:INE-1{}1895
Loc: 23683225-23685577 mobile_element_type = transposon:G4{}2383
Loc: 23685604-23685773 mobile_element_type = transposon:INE-1{}1896
Loc: 23685785-23685853 mobile_element_type = transposon:INE-1{}1897
Loc: 23686205-23686418 mobile_element_type = transposon:F{}4636
Loc: 23686691-23687810 mobile_element_type = transposon:transib3{}2949

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity	ISS Inferred from Sequence or Structural Similarity more info
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables piRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables piRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in piRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in piRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piRNA-mediated retrotransposon silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in secondary piRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in secondary piRNA processing	IGI Inferred from Genetic Interaction more info	PubMed
involved_in secondary piRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in secondary piRNA processing	IPI Inferred from Physical Interaction more info	PubMed
involved_in spermatogenesis	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
is_active_in P granule	IBA Inferred from Biological aspect of Ancestor more info
is_active_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in P granule	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in extrinsic component of mitochondrial outer membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: argonaute 3

Names: AGO3-PD; AGO3-PE; AGO3-PF; AGO3-PG; Argonaute-3; Argonaute3; BEST:LD15785; CG40300-PD; CG40300-PE; CG40300-PF; CG40300-PG

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

23554593..23689640

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001043164.3 → NP_001036629.2 argonaute 3, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036629.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4GND8, A4GUJ7, A7YFW6, B6IDV1, B7FNN2, K7WKR9, K7XHY4, Q7PLK0

Conserved Domains (2) summary

smart00949
Location:291 → 425

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:412 → 850

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...
NM_001043163.3 → NP_001036628.2 argonaute 3, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036628.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4GND8, A4GUJ7, A7YFW6, B6IDV1, B7FNN2, K7WKR9, K7XHY4, Q7PLK0

Conserved Domains (2) summary

smart00949
Location:291 → 425

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:412 → 850

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...
NM_001043162.3 → NP_001036627.2 argonaute 3, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001036627.2

Status: REVIEWED

UniProtKB/Swiss-Prot

A4GND8, A4GUJ7, A7YFW6, B6IDV1, B7FNN2, K7WKR9, K7XHY4, Q7PLK0

Conserved Domains (2) summary

smart00949
Location:291 → 425

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:412 → 850

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...
NM_001170027.2 → NP_001163498.1 argonaute 3, isoform G [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001163498.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q7PLK0

Conserved Domains (2) summary

smart00949
Location:291 → 425

PAZ; This domain is named PAZ after the proteins Piwi Argonaut and Zwille

cd04658
Location:412 → 849

Piwi_piwi-like_Euk; Piwi_piwi-like_Euk: PIWI domain, Piwi-like subfamily found in eukaryotes. This domain is found in Piwi and closely related proteins, where it is believed to perform a crucial role in germline cells, via RNA silencing. RNA silencing refers to a group of ...