nbs1 Mre11 complex BRCT domain subunit Nbs1 [Schizosaccharomyces pombe (fission yeast)] - Gene

Summary

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Official Symbol: nbs1
Official Full Name: Mre11 complex BRCT domain subunit Nbs1
Primary source: PomBase:SPBC6B1.09c
Locus tag: SPOM_SPBC6B1.09C
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Schizosaccharomyces pombe (strain: 972h-)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina; Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae; Schizosaccharomyces
Also known as: slr10; slr8
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Genomic context

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See nbs1 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 7

Sequence:: Chromosome: II; NC_003423.3 (2648577..2650977, complement)

Chromosome II - NC_003423.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003423.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

a mre11 mutant defective in endonucleolytic activity, the same mutant lacking Ctp1, or the triple mutant also lacking the putative hairpin nuclease Pso2 showed wild-type levels of repair. Thus, MRN (Mre11-Rad50-Nbs1)may act to recruit the hairpin opening activity that allows subsequent repair
Title: Nonhomologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe.
these data propose that release of the MRN complex and Ku from DNA ends by Mre11 nuclease activity and Ctp1 is a critical step required to expose ssDNA for replication protein A localization and ensuing homologous recombination repair.
Title: Release of Ku and MRN from DNA ends by Mre11 nuclease activity and Ctp1 is required for homologous recombination repair of double-strand breaks.
Mre11-Rad50-Nbs1 participates in checkpoint-dependent replication slowing through negative regulation of recombination.
Title: The fission yeast Rad32(Mre11)-Rad50-Nbs1 complex acts both upstream and downstream of checkpoint signaling in the S-phase DNA damage checkpoint.
Rad32 nuclease has the catalytic site for Rec12-oligonucleotide generation and is activated by Ctp1, which plays an additional role in meiotic recombination.
Title: Meiotic DNA double-strand break repair requires two nucleases, MRN and Ctp1, to produce a single size class of Rec12 (Spo11)-oligonucleotide complexes.
Tethering of Ctp1 to a flexible Nbs1 arm suggests a mechanism for restricting DNA end processing and homologous recombination activities of Sae2/Ctp1/CtIP to the immediate vicinity of double-strand breaks.
Title: Nbs1 flexibly tethers Ctp1 and Mre11-Rad50 to coordinate DNA double-strand break processing and repair.
Study presents a 2.3A crystal structure of the N-terminal region of fission yeast Nbs1, revealing an unusual but conserved architecture in which the FHA- and BRCT-repeat domains structurally coalesce.
Title: A supramodular FHA/BRCT-repeat architecture mediates Nbs1 adaptor function in response to DNA damage.
Study finds that in the presence of the MRN (Rad32-Rad50-Nbs1) complex efficient recombination requires Ctp1, the ortholog of the nuclease Sae2, but not the nuclease activity of MRN.
Title: Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.
The Mre11-Rad50-Nbs1 S-phase checkpoint role is separate from its Ctp1- and resection-dependent role in double-strand break repair.
Title: The role of MRN in the S-phase DNA damage checkpoint is independent of its Ctp1-dependent roles in double-strand break repair and checkpoint signaling.
This study demonstrates for the first time that Mre11 (Schizosaccharomyces pombe Rad32(Mre11)) nuclease activity is required for the removal of Rec12(Spo11).
Title: Ctp1CtIP and Rad32Mre11 nuclease activity are required for Rec12Spo11 removal, but Rec12Spo11 removal is dispensable for other MRN-dependent meiotic functions.
ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1.
Title: ATM activation and its recruitment to damaged DNA require binding to the C terminus of Nbs1.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by PomBase

Function	Evidence Code	Pubs
enables chromatin-protein adaptor activity	EXP Inferred from Experiment more info	PubMed
enables chromatin-protein adaptor activity	IDA Inferred from Direct Assay more info	PubMed
enables damaged DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphorylation-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA double-strand break processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA duplex unwinding	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via homologous recombination	IBA Inferred from Biological aspect of Ancestor more info
involved_in double-strand break repair via nonhomologous end joining	IGI Inferred from Genetic Interaction more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G2 DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to chromosome, telomeric region	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IDA Inferred from Direct Assay more info	PubMed
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of Mre11 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Mre11 complex	IDA Inferred from Direct Assay more info	PubMed
part_of Mre11 complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in chromosome, telomeric repeat region	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	HDA more info	PubMed
is_active_in site of double-strand break	IDA Inferred from Direct Assay more info	PubMed