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Mid2 midline 2 [ Rattus norvegicus (Norway rat) ]

Gene ID: 363502, updated on 14-Nov-2024

Summary

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Official Symbol
Mid2provided by RGD
Official Full Name
midline 2provided by RGD
Primary source
RGD:1565674
See related
EnsemblRapid:ENSRNOG00000060542 AllianceGenome:RGD:1565674
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Rattus norvegicus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as
RGD1565674
Summary
Predicted to enable several functions, including microtubule binding activity; protein homodimerization activity; and transcription coactivator activity. Predicted to be involved in several processes, including defense response to symbiont; negative regulation of viral transcription; and positive regulation of NF-kappaB transcription factor activity. Predicted to be located in cytoplasm. Predicted to colocalize with microtubule. Human ortholog(s) of this gene implicated in non-syndromic X-linked intellectual disability 101. Orthologous to human MID2 (midline 2). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Biased expression in Lung (RPKM 111.1), Adrenal (RPKM 61.7) and 9 other tissues See more
Orthologs
human mouse all
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Genomic context

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See Mid2 in Genome Data Viewer
Location:
Xq33
Exon count:
11
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCr8 (GCF_036323735.1) X NC_086039.1 (109143057..109245257)
RS_2023_06 previous assembly mRatBN7.2 (GCF_015227675.2) X NC_051356.1 (104354692..104456757)
106 previous assembly Rnor_6.0 (GCF_000001895.5) X NC_005120.4 (112019897..112121980)

Chromosome X - NC_086039.1Genomic Context describing neighboring genes Neighboring gene TSC22 domain family, member 3 Neighboring gene ribosomal protein L23a, pseudogene 9 Neighboring gene U6 spliceosomal RNA Neighboring gene uncharacterized LOC103694504 Neighboring gene ribosomal protein SA, pseudogene 18

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
  • Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
  • BioProject: PRJNA238328
  • Publication: PMID 24510058
  • Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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Related articles in PubMed

  1. MID2, a homologue of the Opitz syndrome gene MID1: similarities in subcellular localization and differences in expression during development. Buchner G, et al. Hum Mol Genet, 1999 Aug. PMID 10400986
  2. MID1 and MID2 homo- and heterodimerise to tether the rapamycin-sensitive PP2A regulatory subunit, alpha 4, to microtubules: implications for the clinical variability of X-linked Opitz GBBB syndrome and other developmental disorders. Short KM, et al. BMC Cell Biol, 2002. PMID 11806752, Free PMC Article
  3. TRIM-mediated precision autophagy targets cytoplasmic regulators of innate immunity. Kimura T, et al. J Cell Biol, 2015 Sep 14. PMID 26347139, Free PMC Article
  4. TRIM protein-mediated regulation of inflammatory and innate immune signaling and its association with antiretroviral activity. Uchil PD, et al. J Virol, 2013 Jan. PMID 23077300, Free PMC Article
  5. TRIM E3 ligases interfere with early and late stages of the retroviral life cycle. Uchil PD, et al. PLoS Pathog, 2008 Feb 8. PMID 18248090, Free PMC Article

See all (8) citations in PubMed

GeneRIFs: Gene References Into Functions

General gene information

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Markers

Homology

Gene Ontology Provided by RGD

Function Evidence Code Pubs
enables enzyme binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables microtubule binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables microtubule binding ISO
Inferred from Sequence Orthology
more info
  
enables phosphoprotein binding ISO
Inferred from Sequence Orthology
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables transcription coactivator activity ISO
Inferred from Sequence Orthology
more info
  
enables transferase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in innate immune response ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of viral entry into host cell ISO
Inferred from Sequence Orthology
more info
  
involved_in negative regulation of viral transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of DNA-binding transcription factor activity ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of DNA-templated transcription IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of NF-kappaB transcription factor activity ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of autophagy ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of canonical NF-kappaB signal transduction ISO
Inferred from Sequence Orthology
more info
  
involved_in protein localization to microtubule IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in protein localization to microtubule ISO
Inferred from Sequence Orthology
more info
  
involved_in suppression of viral release by host ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
colocalizes_with microtubule IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in microtubule ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
probable E3 ubiquitin-protein ligase MID2
NP_001178818.1
XP_006234309.1
XP_006234310.1
XP_063136223.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001191889.2 → NP_001178818.1  probable E3 ubiquitin-protein ligase MID2 isoform 3

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021
    UniProtKB/TrEMBL
    A0A0G2JWJ3
    Related
    ENSRNOP00000069903.2, ENSRNOT00000079841.3
    Conserved Domains (5) summary
    smart00502
    Location:239 → 364
    BBC; B-Box C-terminal domain
    smart00336
    Location:195 → 232
    BBOX; B-Box-type zinc finger
    cd00063
    Location:401 → 531
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd00162
    Location:29 → 83
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    cd13739
    Location:536 → 705
    SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

  2. NM_001434288.1 → NP_001421217.1  probable E3 ubiquitin-protein ligase MID2 isoform 1

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021

  3. NM_001434289.1 → NP_001421218.1  probable E3 ubiquitin-protein ligase MID2 isoform 2

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021

  4. NM_001434290.1 → NP_001421219.1  probable E3 ubiquitin-protein ligase MID2 isoform 4

    Status: VALIDATED

    Source sequence(s)
    JAXUCZ010000021

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

  1. NC_086039.1 Reference GRCr8

    Range
    109143057..109245257
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. XM_063280153.1 → XP_063136223.1  probable E3 ubiquitin-protein ligase MID2 isoform X2

    Related
    ENSRNOP00000101518.1, ENSRNOT00000118902.2

  2. XM_006234248.5 → XP_006234310.1  probable E3 ubiquitin-protein ligase MID2 isoform X3

    Conserved Domains (5) summary
    smart00502
    Location:219 → 344
    BBC; B-Box C-terminal domain
    smart00336
    Location:175 → 212
    BBOX; B-Box-type zinc finger
    cd00063
    Location:381 → 511
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd00162
    Location:9 → 63
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    cd13739
    Location:516 → 685
    SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2

  3. XM_006234247.5 → XP_006234309.1  probable E3 ubiquitin-protein ligase MID2 isoform X1

    UniProtKB/TrEMBL
    A6HLR4
    Conserved Domains (5) summary
    smart00502
    Location:239 → 364
    BBC; B-Box C-terminal domain
    smart00336
    Location:195 → 232
    BBOX; B-Box-type zinc finger
    cd00063
    Location:401 → 501
    FN3; Fibronectin type 3 domain; One of three types of internal repeats found in the plasma protein fibronectin. Its tenth fibronectin type III repeat contains an RGD cell recognition sequence in a flexible loop between 2 strands. Approximately 2% of all ...
    cd00162
    Location:29 → 83
    RING; RING-finger (Really Interesting New Gene) domain, a specialized type of Zn-finger of 40 to 60 residues that binds two atoms of zinc; defined by the 'cross-brace' motif C-X2-C-X(9-39)-C-X(1-3)- H-X(2-3)-(N/C/H)-X2-C-X(4-48)C-X2-C; probably involved in ...
    cd13739
    Location:506 → 675
    SPRY_PRY_TRIM1; PRY/SPRY domain of tripartite motif-binding protein 1 (TRIM1) or MID2
Nucleotide Protein Strain
Heading Accession and Version

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