AGO2 Argonaute 2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: AGO2provided by FlyBase
Official Full Name: Argonaute 2provided by FlyBase
Primary source: FLYBASE:FBgn0087035
Locus tag: Dmel_CG7439
See related: AllianceGenome:FB:FBgn0087035
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: AG02; ago; AGO 2; ago-2; Ago-2; AGO-2; AGO1; ago2; Ago2; Ago2a; argonaute2; CG13452; CG7439; dAgo2; dAGO2; Dm Ago2; DmAgo2; Dmel\CG7439; eIF2C2
Summary: Enables endoribonuclease activity, cleaving siRNA-paired mRNA; siRNA binding activity; and single-stranded RNA binding activity. Involved in several processes, including dsRNA transport; gene silencing by RNA; and response to virus. Located in neuronal ribonucleoprotein granule and nucleus. Part of RISC complex; RISC-loading complex; and messenger ribonucleoprotein complex. Is expressed in adult brain cell body rind and adult head. [provided by Alliance of Genome Resources, Apr 2022]
Orthologs: all
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Genomic context

Location:: 71E1-71E1; 3-43 cM

Exon count:: 8

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3L	NT_037436.4 (15554113..15561042)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3L	NT_037436.3 (15547213..15554142)

Chromosome 3L - NT_037436.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates.
Title: N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates.
Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs during Sperm Maturation in Drosophila.
Title: Modulation of Ago2 Loading by Cyclophilin 40 Endows a Unique Repertoire of Functional miRNAs during Sperm Maturation in Drosophila.
Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation.
Title: Ago2 protects Drosophila siRNAs and microRNAs from target-directed degradation, even in the absence of 2'-O-methylation.
Age-Related Argonaute Loading of Ribosomal RNA Fragments.
Title: Age-Related Argonaute Loading of Ribosomal RNA Fragments.
AGO2 impairs the ability of Pol II to elongate and alters NELF-E recruitment at active promoters.
Title: Argonaute2 attenuates active transcription by limiting RNA Polymerase II elongation in Drosophila melanogaster.
We chose Drosophila as a model system to take advantage of the presence of two biochemically distinct Argonautes, AGO1 and AGO2. Our results revealed AGO2 loading to be strongly favored by G-rich sequences. In contrast, AGO1 showed an enrichment of the 'GAC' motif in loaded species.
Title: Hidden sequence specificity in loading of single-stranded RNAs onto Drosophila Argonautes.
Cricket paralysis virus protein CrPV-1A comprises a BC-box motif to recruit an E3 ubiquitin ligase for Ago-2 degradation.
Title: A Viral Protein Restricts Drosophila RNAi Immunity by Regulating Argonaute Activity and Stability.
Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies.
Title: Conserved association of Argonaute 1 and 2 proteins with miRNA and siRNA pathways throughout insect evolution, from cockroaches to flies.
Results provide evidence for an Ago2-interaction network participating in dosage compensation in drosophila.
Title: Chromatin That Guides Dosage Compensation Is Modulated by the siRNA Pathway in Drosophila melanogaster.
findings reveal coordination of AGO2 and LaminB function to dictate genome architecture and thereby regulate gene expression
Title: Argonaute2 and LaminB modulate gene expression by controlling chromatin topology.

Submit: New GeneRIF Correction See all GeneRIFs (51)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

mobile_element

Loc: 15554974-15556705 mobile_element_type = transposon:S{}AGO2[S1008]

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables RNA endonuclease activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA endonuclease activity, producing 5'-phosphomonoesters	IDA Inferred from Direct Assay more info	PubMed
enables endoribonuclease activity, cleaving siRNA-paired mRNA	IDA Inferred from Direct Assay more info	PubMed
enables miRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNAi-mediated antiviral immune response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in RNAi-mediated antiviral immunity against RNA virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellularization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to virus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in dosage compensation by hyperactivation of X chromosome	IGI Inferred from Genetic Interaction more info	PubMed
involved_in dsRNA transport	IMP Inferred from Mutant Phenotype more info	PubMed
NOT involved_in miRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in miRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of negative regulation of viral genome replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pole cell formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IDA Inferred from Direct Assay more info	PubMed
involved_in regulatory ncRNA-mediated post-transcriptional gene silencing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in segment polarity determination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in siRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in siRNA-mediated gene silencing by mRNA destabilization	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA-mediated gene silencing by mRNA destabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in siRNA-mediated heterochromatin formation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in siRNA-mediated pericentric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in syncytial nuclear migration	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC complex	ISS Inferred from Sequence or Structural Similarity more info
part_of RISC complex	TAS Traceable Author Statement more info	PubMed
part_of RISC-loading complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IGI Inferred from Genetic Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasmic ribonucleoprotein granule	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	HDA more info	PubMed
located_in neuronal ribonucleoprotein granule	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: argonaute 2

Names: AGO2-PB; AGO2-PC; AGO2-PE; ARGONAUTE2; CG7439-PB; CG7439-PC; CG7439-PE; argonaute; argonaute-2

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_037436.4 Reference assembly

Range

15554113..15561042

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_168626.3 → NP_730054.1 argonaute 2, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_730054.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q9VUQ5

Related

FBpp0075313

Conserved Domains (5) summary

PLN03202
Location:411 → 1180

PLN03202; protein argonaute; Provisional

cd02825
Location:605 → 719

PAZ; PAZ domain, named PAZ after the proteins Piwi Argonaut and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the piwi and Dicer families. PAZ functions as a ...

cd04657
Location:764 → 1186

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:554 → 601

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:411 → 543

ArgoN; N-terminal domain of argonaute
NM_140518.3 → NP_648775.1 argonaute 2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_648775.1

Status: REVIEWED

UniProtKB/Swiss-Prot

C7LAD2, Q2Q3Y0, Q2Q3Y1, Q2Q3Y3, Q2Q3Y4, Q2Q3Y5, Q86P07, Q8T3N2, Q9VUQ5, Q9VUQ6

Related

FBpp0075312

Conserved Domains (5) summary

PLN03202
Location:408 → 1177

PLN03202; protein argonaute; Provisional

cd02825
Location:602 → 716

PAZ; PAZ domain, named PAZ after the proteins Piwi Argonaut and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the piwi and Dicer families. PAZ functions as a ...

cd04657
Location:761 → 1183

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:551 → 598

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:408 → 540

ArgoN; N-terminal domain of argonaute
NM_001274953.1 → NP_001261882.1 argonaute 2, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001261882.1

Status: REVIEWED

UniProtKB/TrEMBL

E5KZ94, M9PFK7

Conserved Domains (4) summary

cd02825
Location:175 → 289

PAZ; PAZ domain, named PAZ after the proteins Piwi Argonaut and Zwille. PAZ is found in two families of proteins that are essential components of RNA-mediated gene-silencing pathways, including RNA interference, the piwi and Dicer families. PAZ functions as a ...

cd04657
Location:334 → 756

Piwi_ago-like; Piwi_ago-like: PIWI domain, Argonaute-like subfamily. Argonaute is the central component of the RNA-induced silencing complex (RISC) and related complexes. The PIWI domain is the C-terminal portion of Argonaute and consists of two subdomains, one of ...

pfam08699
Location:124 → 171

ArgoL1; Argonaute linker 1 domain

pfam16486
Location:3 → 113

ArgoN; N-terminal domain of argonaute