Bruce BIR repeat containing ubiquitin-conjugating enzyme [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Bruceprovided by FlyBase
Official Full Name: BIR repeat containing ubiquitin-conjugating enzymeprovided by FlyBase
Primary source: FLYBASE:FBgn0266717
Locus tag: Dmel_CG6303
See related: AllianceGenome:FB:FBgn0266717
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: bruce; BRUCE; CG6303; dbruce; dBruce; dBRUCE; Dmel\CG6303; mod86
Summary: Enables ubiquitin conjugating enzyme activity. Involved in several processes, including germ cell development; negative regulation of hippo signaling; and negative regulation of nurse cell apoptotic process. Predicted to be active in nucleus and trans-Golgi network. Is expressed in adult head; organism; spermatozoon; and testis. Orthologous to human BIRC6 (baculoviral IAP repeat containing 6). [provided by Alliance of Genome Resources, Nov 2024]
Orthologs: all
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Genomic context

Location:: 86A7-86A8; 3-49 cM

Exon count:: 33

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (10312039..10331353, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (6137761..6157075, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

E2 enzyme Bruce negatively regulates Hippo signaling through POSH-mediated expanded degradation.
Title: E2 enzyme Bruce negatively regulates Hippo signaling through POSH-mediated expanded degradation.
dBruce physically interacts with Reaper, dependent upon Reaper's IAP-binding (IBM) and GH3 motifs.
Title: Drosophila BRUCE inhibits apoptosis through non-lysine ubiquitination of the IAP-antagonist REAPER.
Drosophila inhibitor of apoptosis dBruce is degraded by autophagy and this degradation promotes DNA fragmentation and subsequent nurse cell death.
Title: Autophagy as a trigger for cell death: autophagic degradation of inhibitor of apoptosis dBruce controls DNA fragmentation during late oogenesis in Drosophila.
Results reveal autophagic degradation of the IAP protein Bruce as a novel mechanism of triggering cell death and thereby provide a mechanistic link between autophagy and cell death.
Title: Autophagic degradation of dBruce controls DNA fragmentation in nurse cells during late Drosophila melanogaster oogenesis.
Data show that the effector caspase Dcp-1 and the inhibitor of apoptosis protein Bruce function to regulate both autophagy and starvation-induced cell death .
Title: Effector caspase Dcp-1 and IAP protein Bruce regulate starvation-induced autophagy during Drosophila melanogaster oogenesis.
Drosophila Bruce can potently suppress Rpr- and Grim-dependent but not Hid-dependent cell death
Title: Drosophila Bruce can potently suppress Rpr- and Grim-dependent but not Hid-dependent cell death.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Dm1583 (e-PCR)
- UniSTS:45601

Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ubiquitin conjugating enzyme activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin conjugating enzyme activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in apoptotic process	IEA Inferred from Electronic Annotation more info
involved_in cellular response to starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of apoptotic process	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of apoptotic process	ISS Inferred from Sequence or Structural Similarity more info	PubMed
involved_in negative regulation of hippo signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of nurse cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in oogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in programmed cell death	IGI Inferred from Genetic Interaction more info	PubMed
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cytokinesis	IEA Inferred from Electronic Annotation more info
involved_in spermatid development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent protein catabolic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in trans-Golgi network	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: BIR repeat containing ubiquitin-conjugating enzyme

Names: Bruce-PA; Bruce-PB; Bruce-PC; Bruce-PD; Bruce-PE; CG6303-PA; CG6303-PB; CG6303-PC; CG6303-PD; CG6303-PE; dbruce; mod86

NP_001262460.1

EC 2.3.2.23

NP_001262461.1

EC 2.3.2.23

NP_001262462.1

EC 2.3.2.23

NP_001262463.1

EC 2.3.2.23

NP_649995.2

EC 2.3.2.23

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

10312039..10331353 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001275533.2 → NP_001262462.1 BIR repeat containing ubiquitin-conjugating enzyme, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262462.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KGR9

Conserved Domains (3) summary

pfam00179
Location:4535 → 4689

UQ_con; Ubiquitin-conjugating enzyme

pfam00653
Location:251 → 321

BIR; Inhibitor of Apoptosis domain

pfam12356
Location:3438 → 3563

DUF3643; Protein of unknown function (DUF3643)
NM_001275532.2 → NP_001262461.1 BIR repeat containing ubiquitin-conjugating enzyme, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262461.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KH36

Conserved Domains (3) summary

pfam00179
Location:4512 → 4666

UQ_con; Ubiquitin-conjugating enzyme

pfam00653
Location:251 → 321

BIR; Inhibitor of Apoptosis domain

pfam12356
Location:3415 → 3540

DUF3643; Protein of unknown function (DUF3643)
NM_001275534.2 → NP_001262463.1 BIR repeat containing ubiquitin-conjugating enzyme, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262463.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KFQ0

Conserved Domains (3) summary

pfam00179
Location:4525 → 4679

UQ_con; Ubiquitin-conjugating enzyme

pfam00653
Location:251 → 321

BIR; Inhibitor of Apoptosis domain

pfam12356
Location:3439 → 3564

DUF3643; Protein of unknown function (DUF3643)
NM_141738.5 → NP_649995.2 BIR repeat containing ubiquitin-conjugating enzyme, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_649995.2

Status: REVIEWED

UniProtKB/TrEMBL

Q8MUM9, Q9VH01

Related

FBpp0081717

Conserved Domains (3) summary

pfam00179
Location:4536 → 4690

UQ_con; Ubiquitin-conjugating enzyme

pfam00653
Location:251 → 321

BIR; Inhibitor of Apoptosis domain

pfam12356
Location:3439 → 3564

DUF3643; Protein of unknown function (DUF3643)
NM_001275531.2 → NP_001262460.1 BIR repeat containing ubiquitin-conjugating enzyme, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001262460.1

Status: REVIEWED

UniProtKB/TrEMBL

A0A0B4KG50

Conserved Domains (3) summary

pfam00179
Location:4636 → 4790

UQ_con; Ubiquitin-conjugating enzyme

pfam00653
Location:251 → 321

BIR; Inhibitor of Apoptosis domain

pfam12356
Location:3539 → 3664

DUF3643; Protein of unknown function (DUF3643)