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spn-E spindle E [ Drosophila melanogaster (fruit fly) ]

Gene ID: 41919, updated on 18-Sep-2024

Summary

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Official Symbol
spn-Eprovided by FlyBase
Official Full Name
spindle Eprovided by FlyBase
Primary source
FLYBASE:FBgn0003483
Locus tag
Dmel_CG3158
See related
AllianceGenome:FB:FBgn0003483
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Drosophila melanogaster
Lineage
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as
CG3158; Dmel\CG3158; fs(1)hls; fs(3)hls; hes; hls; homeless; spin-E; Spindle-E; spn E; Spn-E; SPN-E; spn-E/hls; spnE; SpnE
Summary
Predicted to enable RNA binding activity and helicase activity. Involved in several processes, including negative regulation of macromolecule metabolic process; oogenesis; and specification of axis polarity. Located in P granule and nucleus. Is expressed in egg chamber; female germline stem cell; germarium; nurse cell; and ovary. Human ortholog(s) of this gene implicated in spermatogenic failure 30. Orthologous to human TDRD9 (tudor domain containing 9). [provided by Alliance of Genome Resources, Apr 2022]
Orthologs
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Genomic context

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See spn-E in Genome Data Viewer
Location:
89A5-89A5; 3-58 cM
Exon count:
11
Annotation release Status Assembly Chr Location
Release 6.54 current Release 6 plus ISO1 MT (GCF_000001215.4) 3R NT_033777.3 (15837419..15844415)
Release 5.57 previous assembly Release 5 (GCF_000001215.2) 3R NT_033777.2 (11663141..11670137)

Chromosome 3R - NT_033777.3Genomic Context describing neighboring genes Neighboring gene Actin-related protein 2/3 complex, subunit 3A Neighboring gene mir-4911 stem loop Neighboring gene NADH dehydrogenase (ubiquinone) 23 kDa subunit Neighboring gene PSEA-binding protein 45kD Neighboring gene crossveinless d Neighboring gene uncharacterized protein Neighboring gene Heavy metal tolerance factor 1

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Interaction of Telomeric Retroelement HeT-A Transcripts and Their Protein Product Gag in Early Embryogenesis of Drosophila. Olovnikov IA, et al. Biochemistry (Mosc), 2016 Sep. PMID 27682174
  2. Selective expansion of the newly evolved genomic variants of retrotransposon 1731 in the Drosophila genomes. Kalmykova AI, et al. Mol Biol Evol, 2004 Dec. PMID 15356284
  3. The GATE retrotransposon in Drosophila melanogaster: mobility in heterochromatin and aspects of its expression in germline tissues. Kogan GL, et al. Mol Genet Genomics, 2003 May. PMID 12756535
  4. Epigenetic Requirements for Triggering Heterochromatinization and Piwi-Interacting RNA Production from Transgenes in the Drosophila Germline. Komarov PA, et al. Cells, 2020 Apr 10. PMID 32290057, Free PMC Article
  5. Subcellular localization and Egl-mediated transport of telomeric retrotransposon HeT-A ribonucleoprotein particles in the Drosophila germline and early embryogenesis. Kordyukova M, et al. PLoS One, 2018. PMID 30157274, Free PMC Article

See all (228) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. The lack of Spn-E caused a significant drop of protein levels of key ping-pong participants, Aubergine (Aub) and AGO3 proteins of PIWI subfamily, in the germline of both males and females, but did not disrupt of their assembly in nuage granules.
    Title: RNA helicase Spn-E is required to maintain Aub and AGO3 protein levels for piRNA silencing in the germline of Drosophila.
  2. DExH box domain of Spindle-E is necessary for its function in the piRNA pathway and retrotransposon silencing during oogenesis and embryonic patterning.
    Title: The DExH box helicase domain of spindle-E is necessary for retrotransposon silencing and axial patterning during Drosophila oogenesis.
  3. RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.
    Title: RNAi is activated during Drosophila oocyte maturation in a manner dependent on aubergine and spindle-E.
  4. mutations in piwi, aubergine, or spindle-E which encode RNAi components result in reduction of H3 Lys9 methylation and delocalization of HP1 and HP2
    Title: Heterochromatic silencing and HP1 localization in Drosophila are dependent on the RNAi machinery.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by FlyBase

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP-dependent activity, acting on RNA TAS
Traceable Author Statement
more info
 PubMed 
enables RNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA helicase activity TAS
Traceable Author Statement
more info
 PubMed 
enables helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables helicase activity ISS
Inferred from Sequence or Structural Similarity
more info
 PubMed 
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in RNA localization TAS
Traceable Author Statement
more info
 PubMed 
involved_in dorsal appendage formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in germarium-derived oocyte fate determination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in global gene silencing by mRNA cleavage IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular mRNA localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in intracellular mRNA localization TAS
Traceable Author Statement
more info
 PubMed 
involved_in mitotic chromosome condensation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in oocyte karyosome formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in oocyte localization involved in germarium-derived egg chamber formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in oocyte maturation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in oogenesis IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in oogenesis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in oogenesis TAS
Traceable Author Statement
more info
 PubMed 
involved_in piRNA-mediated gene silencing by mRNA destabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in piRNA-mediated retrotransposon silencing by mRNA destabilization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in polarity specification of anterior/posterior axis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in polarity specification of dorsal/ventral axis IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in pole plasm assembly NAS
Non-traceable Author Statement
more info
 PubMed 
involved_in regulation of pole plasm oskar mRNA localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in retrotransposon silencing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in secondary piRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in P granule IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm NAS
Non-traceable Author Statement
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
spindle E
Names
CG3158-PA
Spindle-E (homeless)
SpindleE
female sterile(3)homeless
homeless
homeless/spindle-E
spindle-E
spn-E-PA
NP_476741.1

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NT_033777.3 Reference assembly

    Range
    15837419..15844415
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_057393.4 → NP_476741.1  spindle E [Drosophila melanogaster]

    See identical proteins and their annotated locations for NP_476741.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    A2RVD0, Q26453, Q9VF26
    UniProtKB/TrEMBL
    L0CPW3, L0CR04, L0CRC8
    Related
    FBpp0082637
    Conserved Domains (2) summary
    COG1643
    Location:113 → 697
    HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]
    pfam00567
    Location:892 → 1016
    TUDOR; Tudor domain
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9VF26.1 Q9VF26

Gene LinkOut

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