Rhcg Rhesus blood group-associated C glycoprotein [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rhcgprovided by MGI
Official Full Name: Rhesus blood group-associated C glycoproteinprovided by MGI
Primary source: MGI:MGI:1888517
See related: Ensembl:ENSMUSG00000030549 AllianceGenome:MGI:1888517
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Summary: Enables ammonium channel activity. Involved in ammonium transmembrane transport and regulation of pH. Located in apical plasma membrane. Is expressed in medulla oblongata basal plate ventricular layer; renal calyx; and spinal cord ventricular layer. Orthologous to human RHCG (Rh family C glycoprotein). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in genital fat pad adult (RPKM 120.3), kidney adult (RPKM 68.3) and 1 other tissue See more
Orthologs: human all
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Genomic context

Location:: 7 D2; 7 45.09 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	7	NC_000073.7 (79243111..79267405, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	7	NC_000073.6 (79593363..79617657, complement)

Chromosome 7 - NC_000073.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RhCG and H+ATPases are located within the same cellular protein complex in the kidney and this interaction is required for maximal urinary acidification by H+-ATPases, a prerequisite for efficient NH3 secretion and urine excretion of NH4+.
Title: The ammonia transporter RhCG modulates urinary acidification by interacting with the vacuolar proton-ATPases in renal intercalated cells.
We characterized ammonia and ammonium (NH3/NH4 (+)) transport by the rhesus-associated (Rh) glycoproteins RhAG, Rhbg, and Rhcg expressed in Xenopus oocytes. We used ion-selective microelectrodes and two-electrode voltage clamp to measure changes in intracellular pH, surface pH, and whole cell currents induced by NH3/NH4 (+) and methyl amine/ammonium (MA/MA(+)).
Title: Mechanisms of ammonia and ammonium transport by rhesus-associated glycoproteins.
investigated the renal adaptive response of acid excretory pathways to high-protein diets containing normal or low amounts of acid-producing sulfur amino acids (SAA) and examined how this adaption requires the RhCG ammonia transporter
Title: The role of the renal ammonia transporter Rhcg in metabolic responses to dietary protein.
Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) are identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion.
Title: Ammonia transport in the kidney by Rhesus glycoproteins.
Rhcg is critically important in the renal response to metabolic acidosis. in mice with intact Rhcg expression, metabolic acidosis does not induce maximal changes in PEPCK, NHE-3, and GS expression despite the presence of persistent metabolic acidosis.
Title: Effect of collecting duct-specific deletion of both Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) on renal response to metabolic acidosis.
highly expressed in specific cells in the male reproductive tract
Title: Expression of the rhesus glycoproteins, ammonia transporter family members, RHCG and RHBG in male reproductive organs.
hypokalemia increases collecting duct Rhcg expression, and this likely contributes to the hypokalemia-stimulated increase in urinary ammonia excretion, and its adaptive increases in phosphate-dependent glutaminase expression can compensate for the absence of collecting duct Rhcg.
Title: Renal ammonia excretion in response to hypokalemia: effect of collecting duct-specific Rh C glycoprotein deletion.
Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct
Title: Haploinsufficiency of the ammonia transporter Rhcg predisposes to chronic acidosis: Rhcg is critical for apical and basolateral ammonia transport in the mouse collecting duct.
Rhcg expression in intercalated cells is necessary for the normal renal response to metabolic acidosis, contributing to both basal and acidosis-stimulated ammonia excretion. Adaptations in Rhbg expression occur in response to acid-loading.
Title: Effect of intercalated cell-specific Rh C glycoprotein deletion on basal and metabolic acidosis-stimulated renal ammonia excretion.
Rhbg and Rhcg may contribute to bronchial epithelial cell ammonia metabolism and suggest that they do not contribute to pulmonary carbon dioxide transport.
Title: Expression of the gas-transporting proteins, Rh B glycoprotein and Rh C glycoprotein, in the murine lung.

Submit: New GeneRIF Correction See all GeneRIFs (23)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (3)
Targeted (3) 1 citation

General gene information

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Markers

Rhcg (e-PCR), detects polymorphism
- UniSTS:178189

BB065800 (e-PCR)
- UniSTS:185719

Rhcg (e-PCR), detects polymorphism
- UniSTS:546977

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables ammonium channel activity	IBA Inferred from Biological aspect of Ancestor more info
enables ammonium channel activity	IDA Inferred from Direct Assay more info	PubMed
enables ammonium channel activity	ISO Inferred from Sequence Orthology more info
enables ankyrin binding	IEA Inferred from Electronic Annotation more info
enables ankyrin binding	ISO Inferred from Sequence Orthology more info
enables carbon dioxide transmembrane transporter activity	ISO Inferred from Sequence Orthology more info
enables carbon dioxide transmembrane transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in ammonium homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in ammonium transmembrane transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in ammonium transmembrane transport	IDA Inferred from Direct Assay more info	PubMed
involved_in ammonium transmembrane transport	ISO Inferred from Sequence Orthology more info
involved_in carbon dioxide transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in intracellular monoatomic ion homeostasis	IEA Inferred from Electronic Annotation more info
involved_in intracellular monoatomic ion homeostasis	ISO Inferred from Sequence Orthology more info
involved_in regulation of pH	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transepithelial ammonium transport	IEA Inferred from Electronic Annotation more info
involved_in transepithelial ammonium transport	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in apical plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in apical plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in apical plasma membrane	ISO Inferred from Sequence Orthology more info
located_in basal plasma membrane	ISO Inferred from Sequence Orthology more info
is_active_in basolateral plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in basolateral plasma membrane	IEA Inferred from Electronic Annotation more info
located_in basolateral plasma membrane	ISO Inferred from Sequence Orthology more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: ammonium transporter Rh type C

Names: Rh type C glycoprotein; rh family type C glycoprotein; rhesus blood group family type C glycoprotein; rhesus-associated C glycoprotein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_019799.3 → NP_062773.2 ammonium transporter Rh type C

See identical proteins and their annotated locations for NP_062773.2

Status: VALIDATED

Source sequence(s)

AI648871, AK076757

Consensus CDS

CCDS21383.1

UniProtKB/Swiss-Prot

Q8BVS0, Q9QXP0

UniProtKB/TrEMBL

B2RPX8

Related

ENSMUSP00000032766.5, ENSMUST00000032766.5

Conserved Domains (1) summary

cl03012
Location:61 → 436

Ammonium_transp; Ammonium Transporter Family

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000073.7 Reference GRCm39 C57BL/6J

Range

79243111..79267405 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_011250878.4 → XP_011249180.1 ammonium transporter Rh type C isoform X1

UniProtKB/TrEMBL

B2RPX8

Conserved Domains (1) summary

cl03012
Location:39 → 411

Ammonium_transp; Ammonium Transporter Family
XM_006541004.1 → XP_006541067.1 ammonium transporter Rh type C isoform X2

Conserved Domains (1) summary

cl03012
Location:20 → 287

Ammonium_transp; Ammonium Transporter Family