Agap2 ArfGAP with GTPase domain, ankyrin repeat and PH domain 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Agap2provided by RGD
Official Full Name: ArfGAP with GTPase domain, ankyrin repeat and PH domain 2provided by RGD
Primary source: RGD:628844
See related: EnsemblRapid:ENSRNOG00000025584 AllianceGenome:RGD:628844
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: Pike; Centg1
Summary: Enables protein kinase activator activity and protein kinase binding activity. Involved in negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of neuron apoptotic process; and positive regulation of phosphatidylinositol 3-kinase signaling. Located in nucleus. Human ortholog(s) of this gene implicated in prostate cancer. Orthologous to human AGAP2 (ArfGAP with GTPase domain, ankyrin repeat and PH domain 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Biased expression in Brain (RPKM 303.8), Thymus (RPKM 101.0) and 5 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 7q22

Exon count:: 20

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (64782618..64799624)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (62897282..62914295)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (70360199..70377422)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

RACK1 scaffolds AGAP2 to FAK to regulate FAK activity and cell adhesion during the differentiation process.
Title: RACK1 promotes neurite outgrowth by scaffolding AGAP2 to FAK.
PIKE plays pivotal roles to advance oligodendroglia development and myelinogenesis through Akt/mTOR activation.
Title: PIKE is essential for oligodendroglia development and CNS myelination.
PIKE is a critical factor in controlling synaptic AMPA receptor insertion.
Title: PIKE-mediated PI3-kinase activity is required for AMPA receptor surface expression.
Pike/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of nerve growth factor in the cell nucleus.
Title: PIKE/nuclear PI 3-kinase signaling mediates the antiapoptotic actions of NGF in the nucleus.
PIKE-L is an important mediator of merlin growth suppression
Title: Neurofibromatosis 2 (NF2) tumor suppressor merlin inhibits phosphatidylinositol 3-kinase through binding to PIKE-L.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH132545 (e-PCR)
- UniSTS:215828

RH140015 (e-PCR)
- UniSTS:222097

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTP binding	ISO Inferred from Sequence Orthology more info
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info
enables GTPase activator activity	ISO Inferred from Sequence Orthology more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables GTPase activity	ISO Inferred from Sequence Orthology more info
enables ionotropic glutamate receptor binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables phosphatidylinositol 3-kinase regulator activity	ISO Inferred from Sequence Orthology more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein kinase activator activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activator activity	ISO Inferred from Sequence Orthology more info
enables protein kinase binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within actin cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in activation of GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of cell surface receptor signaling pathway via JAK-STAT	ISO Inferred from Sequence Orthology more info
involved_in cellular response to glycine	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within endosomal transport	ISO Inferred from Sequence Orthology more info
acts_upstream_of epithelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within mammary gland alveolus development	ISO Inferred from Sequence Orthology more info
acts_upstream_of mammary gland epithelial cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of epithelial cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of extrinsic apoptotic signaling pathway in absence of ligand	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neuron apoptotic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within negative regulation of neuron apoptotic process	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of protein catabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of mammary gland epithelial cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of protein localization to cell surface	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of receptor signaling pathway via JAK-STAT	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within positive regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Flemming body	IEA Inferred from Electronic Annotation more info
located_in Flemming body	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in cytosol	TAS Traceable Author Statement more info
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in external side of plasma membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in neuron projection	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	ISO Inferred from Sequence Orthology more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
is_active_in postsynapse	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Names: AGAP-2; centaurin, gamma 1; cnt-g1; nuclear GTPase PIKE; phosphatidylinositol 3-kinase enhancer

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_023026.2 → NP_075415.2 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

See identical proteins and their annotated locations for NP_075415.2

Status: VALIDATED

Source sequence(s)

AF280816, AY128688, JAXUCZ010000007

UniProtKB/Swiss-Prot

Q8CGU4, Q9JHW8

UniProtKB/TrEMBL

A6HQS4

Related

ENSRNOP00000109311.1, ENSRNOT00000136987.1

Conserved Domains (6) summary

cd04103
Location:401 → 560

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

PRK07764
Location:37 → 174

PRK07764; DNA polymerase III subunits gamma and tau; Validated

cd01250
Location:668 → 908

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

sd00045
Location:1084 → 1115

ANK; ANK repeat [structural motif]

cd08836
Location:926 → 1033

ArfGap_AGAP; ArfGAP with GTPase domain, ANK repeat and PH domains

pfam12796
Location:1089 → 1140

Ank_2; Ankyrin repeats (3 copies)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

64782618..64799624

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_063264227.1 → XP_063120297.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X2

UniProtKB/TrEMBL

A0A8I6AM98

Related

ENSRNOP00000093674.1, ENSRNOT00000117693.2
XM_006241500.5 → XP_006241562.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X3

Conserved Domains (8) summary

cd04103
Location:71 → 230

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

smart00010
Location:69 → 236

small_GTPase; Small GTPase of the Ras superfamily; ill-defined subfamily

cd00204
Location:727 → 791

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd01250
Location:338 → 558

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

pfam00169
Location:516 → 553

PH; PH domain

pfam01412
Location:577 → 691

ArfGap; Putative GTPase activating protein for Arf

pfam13857
Location:722 → 775

Ank_5; Ankyrin repeats (many copies)

sd00045
Location:734 → 765

ANK; ANK repeat [structural motif]
XM_006241499.5 → XP_006241561.1 arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2 isoform X1

UniProtKB/TrEMBL

A0A0G2JVS4

Related

ENSRNOP00000069597.2, ENSRNOT00000084012.3

Conserved Domains (8) summary

cd04103
Location:401 → 560

Centaurin_gamma; Centaurin gamma (CENTG) GTPase

smart00010
Location:399 → 566

small_GTPase; Small GTPase of the Ras superfamily; ill-defined subfamily

cd00204
Location:1057 → 1121

ANK; ankyrin repeats; ankyrin repeats mediate protein-protein interactions in very diverse families of proteins. The number of ANK repeats in a protein can range from 2 to over 20 (ankyrins, for example). ANK repeats may occur in combinations with other ...

cd01250
Location:668 → 888

PH_AGAP; Arf-GAP with GTPase, ANK repeat and PH domain-containing protein Pleckstrin homology (PH) domain

pfam00169
Location:846 → 883

PH; PH domain

pfam01412
Location:907 → 1021

ArfGap; Putative GTPase activating protein for Arf

pfam13857
Location:1052 → 1105

Ank_5; Ankyrin repeats (many copies)

sd00045
Location:1064 → 1095

ANK; ANK repeat [structural motif]