Pnpla8 patatin-like phospholipase domain containing 8 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Pnpla8provided by MGI
Official Full Name: patatin-like phospholipase domain containing 8provided by MGI
Primary source: MGI:MGI:1914702
See related: Ensembl:ENSMUSG00000036257 AllianceGenome:MGI:1914702
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Ipla2(gamma); 1200006O19Rik
Summary: Enables calcium-independent phospholipase A2 activity. Involved in cardiolipin metabolic process; regulation of cellular response to oxidative stress; and triglyceride homeostasis. Located in mitochondrion and peroxisome. Orthologous to human PNPLA8 (patatin like phospholipase domain containing 8). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in cortex adult (RPKM 9.4), frontal lobe adult (RPKM 9.1) and 25 other tissues See more
Orthologs: human all
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Genomic context

Location:: 12 B2; 12 20.71 cM

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	12	NC_000078.7 (44315916..44362718)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	12	NC_000078.6 (44269132..44315936)

Chromosome 12 - NC_000078.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Hepatic phosphatidylcholine catabolism driven by PNPLA7 and PNPLA8 supplies endogenous choline to replenish the methionine cycle with methyl groups.
Title: Hepatic phosphatidylcholine catabolism driven by PNPLA7 and PNPLA8 supplies endogenous choline to replenish the methionine cycle with methyl groups.
Gene Deletion of Calcium-Independent Phospholipase A2gamma (iPLA2gamma) Suppresses Adipogenic Differentiation of Mouse Embryonic Fibroblasts.
Title: Gene Deletion of Calcium-Independent Phospholipase A(2)γ (iPLA(2)γ) Suppresses Adipogenic Differentiation of Mouse Embryonic Fibroblasts.
Genetic Ablation of Calcium-independent Phospholipase A2gamma Exacerbates Glomerular Injury in Adriamycin Nephrosis in Mice.
Title: Genetic Ablation of Calcium-independent Phospholipase A(2)γ Exacerbates Glomerular Injury in Adriamycin Nephrosis in Mice.
over-expression of PNPLA8 dramatically decreases hepatic steatosis through increased autophagy in hepatocytes of high-fat diet-fed mice
Title: SREBP-2/PNPLA8 axis improves non-alcoholic fatty liver disease through activation of autophagy.
these results demonstrate the integrated roles of Cardiolipin and iPLA2gamma in lipid second-messenger production and mitochondrial bioenergetics during oxidative stress.
Title: The phospholipase iPLA(2)γ is a major mediator releasing oxidized aliphatic chains from cardiolipin, integrating mitochondrial bioenergetics and signaling.
Because mitochondrial membrane depolarization and calcium are known to activate iPLA2gamma, these results are consistent with salvage of myocardium after Ischemia/Reperfusion by iPLA2gamma loss of function through decreasing mPTP opening, diminishing production of proinflammatory oxidized fatty acids
Title: Cardiac Myocyte-specific Knock-out of Calcium-independent Phospholipase A2γ (iPLA2γ) Decreases Oxidized Fatty Acids during Ischemia/Reperfusion and Reduces Infarct Size.
UCP2, functional due to fatty acids released by redox-activated mt-iPLA2gamma, suppresses mitochondrial superoxide production by its uncoupling action.
Title: Antioxidant activity by a synergy of redox-sensitive mitochondrial phospholipase A2 and uncoupling protein-2 in lung and spleen.
Mitochondrial dysfunction and reduced prostaglandin synthesis in skeletal muscle of Group VIB Ca2+-independent phospholipase A2gamma-deficient mice
Title: Mitochondrial dysfunction and reduced prostaglandin synthesis in skeletal muscle of Group VIB Ca2+-independent phospholipase A2gamma-deficient mice.
the obligatory role of iPLA(2)gamma in neuronal mitochondrial lipid metabolism and membrane structure demonstrating that iPLA(2)gamma loss of function results in a mitochondrial neurodegenerative disorder
Title: Genetic ablation of calcium-independent phospholipase A2{gamma} leads to alterations in hippocampal cardiolipin content and molecular species distribution, mitochondrial degeneration, autophagy, and cognitive dysfunction.
iPLA(2)gamma is essential for maintaining efficient bioenergetic mitochondrial function through tailoring mitochondrial membrane lipid metabolism and composition
Title: Genetic ablation of calcium-independent phospholipase A2gamma leads to alterations in mitochondrial lipid metabolism and function resulting in a deficient mitochondrial bioenergetic phenotype.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Gene trapped (1)
Targeted (1) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables calcium-independent phospholipase A2 activity	IBA Inferred from Biological aspect of Ancestor more info
enables calcium-independent phospholipase A2 activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables calcium-independent phospholipase A2 activity	ISO Inferred from Sequence Orthology more info
enables calcium-independent phospholipase A2 activity	ISS Inferred from Sequence or Structural Similarity more info
enables lysophospholipase activity	IEA Inferred from Electronic Annotation more info
enables phospholipase A1 activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in arachidonate metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in arachidonate metabolic process	ISO Inferred from Sequence Orthology more info
involved_in arachidonate metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in arachidonate secretion	IEA Inferred from Electronic Annotation more info
involved_in arachidonate secretion	ISO Inferred from Sequence Orthology more info
involved_in cardiolipin metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cardiolipin metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fatty acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in fatty acid metabolic process	ISS Inferred from Sequence or Structural Similarity more info
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	ISO Inferred from Sequence Orthology more info
involved_in linoleic acid metabolic process	IEA Inferred from Electronic Annotation more info
involved_in linoleic acid metabolic process	ISO Inferred from Sequence Orthology more info
involved_in lipid homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lipid homeostasis	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylcholine catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylcholine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylethanolamine catabolic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylethanolamine catabolic process	ISO Inferred from Sequence Orthology more info
involved_in prostaglandin biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in prostaglandin biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in regulation of cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in triglyceride homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in triglyceride homeostasis	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	ISO Inferred from Sequence Orthology more info
located_in membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in mitochondrial membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisomal membrane	ISO Inferred from Sequence Orthology more info
located_in peroxisomal membrane	ISS Inferred from Sequence or Structural Similarity more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: calcium-independent phospholipase A2-gamma

Names: iPLA2-gamma; intracellular membrane-associated calcium-independent phospholipase A2 gamma; patatin-like phospholipase domain-containing protein 8

NP_080440.2

EC 3.1.1.5

XP_006516237.1

EC 3.1.1.5

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_026164.2 → NP_080440.2 calcium-independent phospholipase A2-gamma

See identical proteins and their annotated locations for NP_080440.2

Status: VALIDATED

Source sequence(s)

AB044139, AK033938, AK145776, CJ112890

Consensus CDS

CCDS36436.1

UniProtKB/Swiss-Prot

Q3TH33, Q8K1N1, Q8VEC0, Q9DC20

UniProtKB/TrEMBL

E9QAC9

Related

ENSMUSP00000043286.9, ENSMUST00000043082.16

Conserved Domains (1) summary

cd07211
Location:429 → 735

Pat_PNPLA8; Patatin-like phospholipase domain containing protein 8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000078.7 Reference GRCm39 C57BL/6J

Range

44315916..44362718

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006516174.5 → XP_006516237.1 calcium-independent phospholipase A2-gamma isoform X1

UniProtKB/TrEMBL

E9QAC9

Conserved Domains (1) summary

cd07211
Location:429 → 685

Pat_PNPLA8; Patatin-like phospholipase domain containing protein 8