Nell2 neural EGFL like 2 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nell2provided by RGD
Official Full Name: neural EGFL like 2provided by RGD
Primary source: RGD:620999
See related: EnsemblRapid:ENSRNOG00000006235 AllianceGenome:RGD:620999
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Summary: Enables heparin binding activity; identical protein binding activity; and protein kinase C binding activity. Involved in several processes, including development of secondary female sexual characteristics; neuron cellular homeostasis; and positive regulation of hormone secretion. Located in several cellular components, including dendrite; extracellular space; and perikaryon. Orthologous to human NELL2 (neural EGFL like 2). [provided by Alliance of Genome Resources, Apr 2022]
Expression: Restricted expression toward (RPKM 754.9) See more
Orthologs: human mouse all
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Genomic context

Location:: 7q35

Exon count:: 22

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	7	NC_086025.1 (128222333..128542127, complement)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	7	NC_051342.1 (126343089..126662903, complement)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	7	NC_005106.4 (136526497..136853820, complement)

Chromosome 7 - NC_086025.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression.
Title: Transcriptional Regulatory Role of NELL2 in Preproenkephalin Gene Expression.
NELL2 Function in Axon Development of Hippocampal Neurons.
Title: NELL2 Function in Axon Development of Hippocampal Neurons.
Report shows that neural tissue-enriched multifunctional protein, NELL2 is a regulatory component of feeding behavior, possibly through a modulation of neuronal activity in the hypothalamus.
Title: A Role of Central NELL2 in the Regulation of Feeding Behavior in Rats.
Data indicate that RNA interference of NEL-like protein 2 (NELL2) reduced NELL2 and gonadotropin-releasing hormone (GnRH) expression at multiple stages of sexual development.
Title: Effects of NELL2 on the regulation of GnRH expression and puberty in female rats.
Results suggest that the N-terminal region of NELL2 determines both the pattern of its intracellular expression and transport and may affect the cellular activity of cells in a paracrine or autocrine manner.
Title: The molecular mechanism of NELL2 movement and secretion in hippocampal progenitor HiB5 cells.
It was shown that Nell2 expression at both mRNA and protein levels is localized to retinal ganglion cells. Nell2 had a molecular weight of ~ 140 kDa and 90 kDa, corresponding to the glycosylated and non-glycosylated form, respectively.
Title: The neuronal EGF-related gene Nell2 interacts with Macf1 and supports survival of retinal ganglion cells after optic nerve injury.
involved in the maintenance of the normal female reproductive cycle
Title: Regulation of the female rat estrous cycle by a neural cell-specific epidermal growth factor-like repeat domain containing protein, NELL2.
These results suggest that NELL2-Tsp,a novel alternative splice variant of rat NELL2, may act as a negative regulator of wild-type NELL2.
Title: Identification and characterization of a truncated isoform of NELL2.
NELL2 is a new component of glutamatergic neurons and is involved in a major, glutamate-dependent, process of neuroendocrine regulation.
Title: NELL2, a neuron-specific EGF-like protein, is selectively expressed in glutamatergic neurons and contributes to the glutamatergic control of GnRH neurons at puberty.
highest levels of NELL2 were detected in the hippocampus and the cerebellum. In the cerebellar cortex NELL2 was observed only in the GABAergic Purkinje cells not in the excitatory granular cells.
Title: Region- and neuronal phenotype-specific expression of NELL2 in the adult rat brain.

Submit: New GeneRIF Correction See all GeneRIFs (13)

General gene information

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Markers

D7Got112 (e-PCR)
- UniSTS:114763

BF420438 (e-PCR)
- UniSTS:248662

BI277674 (e-PCR)
- UniSTS:243820

RH130208 (e-PCR)
- UniSTS:213495

RH130209 (e-PCR)
- UniSTS:213496

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables calcium ion binding	IEA Inferred from Electronic Annotation more info
enables calcium ion binding	ISO Inferred from Sequence Orthology more info
enables calcium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables heparin binding	IBA Inferred from Biological aspect of Ancestor more info
enables heparin binding	IDA Inferred from Direct Assay more info	PubMed
enables identical protein binding	IEA Inferred from Electronic Annotation more info
enables identical protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables identical protein binding	ISO Inferred from Sequence Orthology more info
enables protein kinase C binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase C binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in commissural neuron axon guidance	IEA Inferred from Electronic Annotation more info
involved_in commissural neuron axon guidance	ISO Inferred from Sequence Orthology more info
involved_in commissural neuron axon guidance	ISS Inferred from Sequence or Structural Similarity more info
involved_in development of secondary female sexual characteristics	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in fertilization	ISO Inferred from Sequence Orthology more info
involved_in fertilization	ISS Inferred from Sequence or Structural Similarity more info
involved_in neurogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in neuron cellular homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of hormone secretion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of growth	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in dendrite	IDA Inferred from Direct Assay more info	PubMed
located_in extracellular region	ISO Inferred from Sequence Orthology more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in perikaryon	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: protein kinase C-binding protein NELL2

Names: NEL-like 2; NEL-like protein 2; rCG50753-like

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031070.3 → NP_112332.2 protein kinase C-binding protein NELL2 precursor

See identical proteins and their annotated locations for NP_112332.2

Status: VALIDATED

Source sequence(s)

JAXUCZ010000007

UniProtKB/Swiss-Prot

A1E5S7, C8CB44, Q561K2, Q62918, Q8R417

UniProtKB/TrEMBL

A0AAA9YW69

Related

ENSRNOP00000008985.5, ENSRNOT00000008985.10

Conserved Domains (6) summary

smart00179
Location:558 → 592

EGF_CA; Calcium-binding EGF-like domain

smart00210
Location:32 → 217

TSPN; Thrombospondin N-terminal -like domains

pfam07645
Location:605 → 636

EGF_CA; Calcium-binding EGF domain

pfam12947
Location:489 → 524

EGF_3; EGF domain

cl00057
Location:438 → 480

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...

cl17735
Location:277 → 333

VWC; von Willebrand factor type C domain

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086025.1 Reference GRCr8

Range

128222333..128542127 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_008765798.2 → XP_008764020.1 protein kinase C-binding protein NELL2 isoform X1

See identical proteins and their annotated locations for XP_008764020.1

UniProtKB/Swiss-Prot

A1E5S7, C8CB44, Q561K2, Q62918, Q8R417

UniProtKB/TrEMBL

A0AAA9YW69

Related

ENSRNOP00000098277.2, ENSRNOT00000120642.2

Conserved Domains (6) summary

smart00179
Location:558 → 592

EGF_CA; Calcium-binding EGF-like domain

smart00210
Location:32 → 217

TSPN; Thrombospondin N-terminal -like domains

pfam07645
Location:605 → 636

EGF_CA; Calcium-binding EGF domain

pfam12947
Location:489 → 524

EGF_3; EGF domain

cl00057
Location:438 → 480

vWFA; Von Willebrand factor type A (vWA) domain was originally found in the blood coagulation protein von Willebrand factor (vWF). Typically, the vWA domain is made up of approximately 200 amino acid residues folded into a classic a/b para-rossmann type of ...

cl17735
Location:277 → 333

VWC; von Willebrand factor type C domain