HMA2 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: HMA2
Primary source: Araport:AT4G30110
Locus tag: AT4G30110
See related: TAIR:AT4G30110
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: ARABIDOPSIS HEAVY METAL ATPASE 2; ATHMA2; F6G3.140; F6G3_140; heavy metal atpase 2
Summary: encodes a protein similar to Zn-ATPase, a P1B-type ATPases transport zinc
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Genomic context

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See HMA2 in Genome Data Viewer

Location:: chromosome: 4

Exon count:: 10

Sequence:: Chromosome: 4; NC_003075.7 (14720062..14725311, complement)

Chromosome 4 - NC_003075.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003075.7

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Arabidopsis thaliana Zn2+-efflux ATPases HMA2 and HMA4 are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina BMM.
Title: Arabidopsis thaliana Zn2+-efflux ATPases HMA2 and HMA4 are required for resistance to the necrotrophic fungus Plectosphaerella cucumerina BMM.
HMA2 and MTP2 act together in Zn deficiency.
Title: Systemic Upregulation of MTP2- and HMA2-Mediated Zn Partitioning to the Shoot Supplements Local Zn Deficiency Responses.
To test whether developing Arabidopsis seeds depend on AtHMA2 and AtHMA4 for proper zinc unloading from the seed coat, the authors analysed the amount of zinc present in the maternal and filial parts of the seed, respectively, in wild-type and mutant seeds lacking functional AtHMA2, AtHMA4, or both.
Title: Mother-plant-mediated pumping of zinc into the developing seed.
The molecular structures of zinc-binding- HMA4 and -HMA7 proteins have been determined and compared in order to provide insights into the structural basis for the differences in their metal binding properties.
Title: Metal binding affinities of Arabidopsis zinc and copper transporters: selectivities match the relative, but not the absolute, affinities of their amino-terminal domains.
observations suggest that the N-terminal domain of HMA2 is essential for function in planta while the C-terminal domain, although not essential for function, may contain a signal important for the subcellular localization of the protein.
Title: Functional analysis of the heavy metal binding domains of the Zn/Cd-transporting ATPase, HMA2, in Arabidopsis thaliana.
The near-complete abolition of root-to-shoot Cd translocation resulting from the loss of function of HMA2 and HMA4 demonstrates they are the major mechanism for Cd translocation in Arabidopsis thaliana.
Title: HMA P-type ATPases are the major mechanism for root-to-shoot Cd translocation in Arabidopsis thaliana.
plant Zn2+-ATPase N-terminal metal binding domains (N-MBDs) have folding & function similar to Cu+-ATPase N-MBDs. unique Zn2+ coordination via two thiols and a carboxyl group provides selective binding of the activating metals to these regulatory domains
Title: Novel Zn2+ coordination by the regulatory N-terminus metal binding domain of Arabidopsis thaliana Zn(2+)-ATPase HMA2.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001341993.1

Supported by ESTs:

NM_119157.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables ABC-type cadmium transporter activity	ISS Inferred from Sequence or Structural Similarity more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables P-type cadmium transporter activity	IEA Inferred from Electronic Annotation more info
enables P-type zinc transporter activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within intracellular zinc ion homeostasis	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: heavy metal atpase 2

NP_001320088.1

heavy metal atpase 2 (HMA2); CONTAINS InterPro DOMAIN/s: Heavy metal transport/detoxification protein (InterPro:IPR006121), ATPase, P-type, ATPase-associated domain (InterPro:IPR008250), ATPase, P-type, heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating (InterPro:IPR006404), ATPase, P-type, H+ transporting proton pump (InterPro:IPR000695), Haloacid dehalogenase-like hydrolase (InterPro:IPR005834), ATPase, P-type, K/Mg/Cd/Cu/Zn/Na/Ca/Na/H-transporter (InterPro:IPR001757), ATPase, P-type, heavy metal translocating (InterPro:IPR006416), ATPase, P-type phosphorylation site (InterPro:IPR018303); BEST Arabidopsis thaliana protein match is: heavy metal atpase 4 (TAIR:AT2G19110.1); Has 39991 Blast hits to 32019 proteins in 3203 species: Archae - 837; Bacteria - 26240; Metazoa - 4077; Fungi - 2516; Plants - 2059; Viruses - 5; Other Eukaryotes - 4257 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003075.7 Reference assembly

Range

14720062..14725311 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001341993.1 → NP_001320088.1 heavy metal atpase 2 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8LPW1, Q9SZW4

UniProtKB/TrEMBL

A0A178UVQ4, A0A5S9XZD2, A0A654FU59, A0A7G2F4X3
NM_119157.4 → NP_194740.1 heavy metal atpase 2 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_194740.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q8LPW1, Q9SZW4

UniProtKB/TrEMBL

A0A178UVQ4, A0A5S9XZD2, A0A654FU59, A0A7G2F4X3

Conserved Domains (4) summary

COG2608
Location:6 → 68

CopZ; Copper chaperone CopZ [Inorganic ion transport and metabolism]

COG4087
Location:525 → 607

COG4087; Soluble P-type ATPase [General function prediction only]

TIGR01512
Location:140 → 695

ATPase-IB2_Cd; heavy metal-(Cd/Co/Hg/Pb/Zn)-translocating P-type ATPase

pfam00122
Location:161 → 380

E1-E2_ATPase; E1-E2 ATPase