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EIR1 Auxin efflux carrier family protein [ Arabidopsis thaliana (thale cress) ]

Gene ID: 835813, updated on 18-Sep-2024

Summary

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Official Symbol
EIR1
Official Full Name
Auxin efflux carrier family protein
Primary source
TAIR:AT5G57090
Locus tag
AT5G57090
See related
Araport:AT5G57090
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Arabidopsis thaliana (ecotype: Columbia)
Lineage
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as
AGR; AGR1; AGRAVITROPIC ROOT; AGRAVITROPIC ROOT 1; ARABIDOPSIS THALIANA PIN-FORMED 2; ATPIN2; AUXIN TRANSPORT PROTEIN EIR1; ETHYLENE INSENSITIVE ROOT 1; MM31; MUL3.3; MUL3_3; PIN-FORMED 2; PIN2; POLAR-AUXIN-TRANSPORT EFFLUX COMPONENT AGRAVITROPIC 1; WAV6; WAVY ROOTS 6
Summary
Encodes an auxin efflux carrier that is similar to bacterial membrane transporters. Root-specific role in the transport of auxin. Acts downstream of CTR1 and ethylene biosynthesis, in the same pathway as EIN2 and AUX1, and independent from EIN3 and EIN5/AIN1 pathway. In the root, the protein localizes apically in epidermal and lateral root cap cells and predominantly basally in cortical cells. Functions may be regulated by phosphorylation status. EIR1 expression is induced by brassinolide treatment in the brassinosteroid-insensitive br1 mutant. Gravistimulation resulted in asymmetric PIN2 distribution, with more protein degraded at the upper side of the gravistimulated root. Protein turnover is affected by the proteasome and by endosomal cycling. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. Membrane sterol composition is essential for the acquisition of PIN2 polarity.
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Genomic context

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See EIR1 in Genome Data Viewer
Location:
chromosome: 5
Exon count:
9
Sequence:
Chromosome: 5; NC_003076.8 (23100516..23105228)

Chromosome 5 - NC_003076.8Genomic Context describing neighboring genes Neighboring gene hydroxyproline-rich glycoprotein family protein Neighboring gene uncharacterized protein Neighboring gene Nucleotide/sugar transporter family protein Neighboring gene autoinhibited Ca2+ -ATPase

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_003076.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. PIN2 turnover in Arabidopsis root epidermal cells explored by the photoconvertible protein Dendra2. Jásik J, et al. PLoS One, 2013. PMID 23637828, Free PMC Article
  2. Evolutionary Conserved Cysteines Function as cis-Acting Regulators of Arabidopsis PIN-FORMED 2 Distribution. Retzer K, et al. Int J Mol Sci, 2017 Oct 29. PMID 29109378, Free PMC Article
  3. Effects of Auxins on PIN-FORMED2 (PIN2) Dynamics Are Not Mediated by Inhibiting PIN2 Endocytosis. Jásik J, et al. Plant Physiol, 2016 Oct. PMID 27506239, Free PMC Article
  4. The E3 ubiquitin ligase SCFTIR1/AFB and membrane sterols play key roles in auxin regulation of endocytosis, recycling, and plasma membrane accumulation of the auxin efflux transporter PIN2 in Arabidopsis thaliana. Pan J, et al. Plant Cell, 2009 Feb. PMID 19218398, Free PMC Article
  5. Tricho- and atrichoblast cell files show distinct PIN2 auxin efflux carrier exploitations and are jointly required for defined auxin-dependent root organ growth. Löfke C, et al. J Exp Bot, 2015 Aug. PMID 26041320, Free PMC Article

See all (77) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis.
    Title: Polarly localized Bro1 domain proteins regulate PIN-FORMED abundance and root gravitropic growth in Arabidopsis.
  2. Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.
    Title: Nonspecific phospholipases C3 and C4 interact with PIN-FORMED2 to regulate growth and tropic responses in Arabidopsis.
  3. Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport.
    Title: Modulation of plant root growth by nitrogen source-defined regulation of polar auxin transport.
  4. The Arabidopsis STE20/Hippo kinase SIK1 regulates polarity independently of PIN proteins.
    Title: The Arabidopsis STE20/Hippo kinase SIK1 regulates polarity independently of PIN proteins.
  5. The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis.
    Title: The AtCRK5 Protein Kinase Is Required to Maintain the ROS NO Balance Affecting the PIN2-Mediated Root Gravitropic Response in Arabidopsis.
  6. Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.
    Title: Analysis of Membrane Proteins Transport from Endosomal Compartments to Vacuoles.
  7. Glucose-TOR signaling regulates PIN2 stability to orchestrate auxin gradient and cell expansion in Arabidopsis root.
    Title: Glucose-TOR signaling regulates PIN2 stability to orchestrate auxin gradient and cell expansion in Arabidopsis root.
  8. Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.
    Title: Salicylic Acid Targets Protein Phosphatase 2A to Attenuate Growth in Plants.
  9. Nickel Toxicity Targets Cell Wall-Related Processes and PIN2-Mediated Auxin Transport to Inhibit Root Elongation and Gravitropic Responses in Arabidopsis.
    Title: Nickel Toxicity Targets Cell Wall-Related Processes and PIN2-Mediated Auxin Transport to Inhibit Root Elongation and Gravitropic Responses in Arabidopsis.
  10. The polar distribution of PIN2 (also known as EIR 1) is influenced by PLC2 in the course of auxin-mediated root development in Arabidopsis
    Title: Phosphatidylinositol-specific phospholipase C2 functions in auxin-modulated root development.
Submit: New GeneRIF Correction See all GeneRIFs (62)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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NM_001345238.1
NM_125091.4

Gene Ontology Provided by TAIR

Function Evidence Code Pubs
enables auxin efflux transmembrane transporter activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables identical protein binding ISS
Inferred from Sequence or Structural Similarity
more info
  
enables protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables protein homodimerization activity ISS
Inferred from Sequence or Structural Similarity
more info
  
Process Evidence Code Pubs
acts_upstream_of_or_within auxin export across the plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
acts_upstream_of_or_within auxin polar transport IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within positive gravitropism IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to auxin IMP
Inferred from Mutant Phenotype
more info
 PubMed 
acts_upstream_of_or_within response to ethylene IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in response to glucose IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within response to hypoxia IEP
Inferred from Expression Pattern
more info
 PubMed 
acts_upstream_of_or_within transmembrane transport IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in transmembrane transport IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
located_in basal part of cell IDA
Inferred from Direct Assay
more info
 PubMed 
located_in basal plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 
located_in chloroplast ISM
Inferred from Sequence Model
more info
  
located_in lytic vacuole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in plasma membrane IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
Auxin efflux carrier family protein
NP_568848.1
  • ETHYLENE INSENSITIVE ROOT 1 (EIR1); CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT2G01420.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_003076.8 Reference assembly

    Range
    23100516..23105228
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_125091.4 → NP_568848.1  Auxin efflux carrier family protein [Arabidopsis thaliana]

    See identical proteins and their annotated locations for NP_568848.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    O82810, Q9LU77, Q9SYT2
    UniProtKB/TrEMBL
    A0A178UL78, A0A384LBE9, A0A5S9YES6, Q67YW0
    Conserved Domains (2) summary
    pfam03547
    Location:9 → 190
    Mem_trans; Membrane transport protein
    cl22894
    Location:486 → 646
    Mem_trans; Membrane transport protein

  2. NM_001345238.1 → NP_001331451.1  Auxin efflux carrier family protein [Arabidopsis thaliana]

    Status: REVIEWED

    UniProtKB/TrEMBL
    A0A1P8BDV9, A0A5S9YES6
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q9LU77.2

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