Nsfl1c NSFL1 cofactor [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Nsfl1cprovided by RGD
Official Full Name: NSFL1 cofactorprovided by RGD
Primary source: RGD:619952
See related: EnsemblRapid:ENSRNOG00000008604 AllianceGenome:RGD:619952
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: p47; XY40
Summary: Enables ATPase binding activity and ubiquitin binding activity. Contributes to phospholipid binding activity. Involved in Golgi organization and membrane fusion. Located in cytosol and spindle pole centrosome. Part of VCP-NSFL1C complex. Orthologous to human NSFL1C (NSFL1 cofactor). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Heart (RPKM 311.9), Muscle (RPKM 288.7) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 3q41

Exon count:: 10

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	3	NC_086021.1 (160438708..160463261)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	3	NC_051338.1 (139978306..140002876)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	3	NC_005102.4 (146980923..147005478)

Chromosome 3 - NC_086021.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest that NADPH oxidase-derived cytosolic superoxide is increased in islets upon oleate infusion and leads to beta cell dysfunction; whole-body NADPH oxidase inhibition (via apocynin) decreases superoxide and restores islet function.
Title: NADPH oxidase inhibition prevents beta cell dysfunction induced by prolonged elevation of oleate in rodents.
In the kidneys of bile duct ligated rats melatonin inhibits protein kinase C beta to reduce p47phox superoxide production.
Title: Melatonin regulates L-arginine transport and NADPH oxidase in young rats with bile duct ligation: role of protein kinase C.
p47(F253S) could not form a complex with p97 and did not cause any cisternal regrowth in an in vitro Golgi reassembly assay.
Title: Isolation of a point-mutated p47 lacking binding affinity to p97ATPase.
Taken together with the previous reports about the function of SHP on gluconeogenesis, our results indicate that SHP can mediate the acute antigluconeogenic effect of PPARgamma.
Title: Transcriptional activation of SHP by PPAR-gamma in liver.
fusion between mitotic Golgi membranes induced by adding cytosol or purified p97/p47 is modulated by phosphatidylethanolamine
Title: Phospholipid species act as modulators in p97/p47-mediated fusion of Golgi membranes.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

WI-20603 (e-PCR)
- UniSTS:23761

RH142919 (e-PCR)
- UniSTS:232166

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATPase binding	IPI Inferred from Physical Interaction more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
contributes_to phospholipid binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in Golgi organization	IDA Inferred from Direct Assay more info	PubMed
involved_in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of mitotic spindle orientation	ISO Inferred from Sequence Orthology more info
involved_in membrane fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in membrane fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in negative regulation of protein localization to centrosome	ISO Inferred from Sequence Orthology more info
involved_in nuclear membrane reassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of mitotic centrosome separation	ISO Inferred from Sequence Orthology more info
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
located_in Golgi stack	IEA Inferred from Electronic Annotation more info
part_of VCP-NSFL1C complex	IEA Inferred from Electronic Annotation more info
part_of VCP-NSFL1C complex	IPI Inferred from Physical Interaction more info	PubMed
part_of VCP-NSFL1C complex	ISO Inferred from Sequence Orthology more info
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	NAS Non-traceable Author Statement more info	PubMed
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole centrosome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: NSFL1 cofactor p47

Names: NSFL1 (p97) cofactor (p47); XY body-associated protein XY40; p97 cofactor p47

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_031981.2 → NP_114187.1 NSFL1 cofactor p47

See identical proteins and their annotated locations for NP_114187.1

Status: PROVISIONAL

Source sequence(s)

AB002086

UniProtKB/Swiss-Prot

O35987

UniProtKB/TrEMBL

A0A0G2K911, A6KHI1

Related

ENSRNOP00000074814.2, ENSRNOT00000090211.3

Conserved Domains (3) summary

smart00553
Location:178 → 270

SEP; Domain present in Saccharomyces cerevisiae Shp1, Drosophila melanogaster eyes closed gene (eyc), and vertebrate p47

cd14348
Location:7 → 45

UBA_p47; UBA-like domain found in NSFL1 cofactor p47 and similar proteins

cd17162
Location:289 → 370

UBX_UBXN2C; Ubiquitin regulatory domain X (UBX) found in NSFL1 cofactor (also known as UBX domain-containing protein 2C (UBXN2C) and similar proteins