UBC35 ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana (thale cress)] - Gene

Summary

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Official Symbol: UBC35
Official Full Name: ubiquitin-conjugating enzyme 35
Primary source: TAIR:AT1G78870
Locus tag: AT1G78870
See related: Araport:AT1G78870
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Arabidopsis thaliana (ecotype: Columbia)
Lineage: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis
Also known as: F9K20.8; F9K20_8; UBC13A; UBIQUITIN CONJUGATING ENZYME 13A; ubiquitin-conjugating enzyme 35
Summary: UBC35/UBC13A encodes a protein that may play a role in DNA damage responses and error-free post-replicative DNA repair by participating in lysine-63-based polyubiquitination reactions. UBC35/UBC13A can form diubiquitin and triubiquitin chains in combination with MMZ1,2,3,4/(UEV1A,B,C,D) in vitro. It can also functionally complement an mms2 ubc13 mutation in budding yeast by increasing the double mutant's viability in the presence of the DNA damaging agent MMS, when it is co-expressed with MMZ / UEV1 genes. A wild type phenotype is restored with MMZ3/UEV1C and MMZ4/UEV1D, but only partial complementation is achieved with MMZ1/UEV1A or MMZ2/UEV1B.
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Genomic context

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See UBC35 in Genome Data Viewer

Location:: chromosome: 1

Exon count:: 7

Sequence:: Chromosome: 1; NC_003070.9 (29650376..29652627)

Chromosome 1 - NC_003070.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_003070.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The results revealed that UBC13 is a key regulator in plant response to low temperature and pathogens.
Title: Arabidopsis UBC13 differentially regulates two programmed cell death pathways in responses to pathogen and low-temperature stress.
UBC13 activity is critical for all major aspects of root development. The double mutant plants were insensitive to auxin treatments and the ubc13 mutant had a reduced auxin response.
Title: UBC13, an E2 enzyme for Lys63-linked ubiquitination, functions in root development by affecting auxin signaling and Aux/IAA protein stability.
Arabidopsis contains two highly conserved and likely duplicated UBC13 genes, AtUBC13A and AtUBC13B. Data suggests the existence of an error-free DNA damage tolerance pathway in plants. [UBC13A]
Title: Arabidopsis thaliana UBC13: implication of error-free DNA damage tolerance and Lys63-linked polyubiquitylation in plants.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

NM_001036221.3

Supported by ESTs:

NM_001334850.1

Supported by ESTs:

NM_001334851.1

Supported by ESTs:

NM_106535.4

Supported by ESTs:

Gene Ontology Provided by TAIR

Function	Evidence Code	Pubs
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables ubiquitin conjugating enzyme activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin-protein transferase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
acts_upstream_of_or_within postreplication repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within response to iron ion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within root epidermal cell differentiation	IGI Inferred from Genetic Interaction more info	PubMed
acts_upstream_of_or_within ubiquitin-dependent protein catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of UBC13-MMS2 complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chloroplast	ISM Inferred from Sequence Model more info
located_in mitochondrion	HDA more info	PubMed
located_in nucleus	ISM Inferred from Sequence Model more info

General protein information

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Preferred Names: ubiquitin-conjugating enzyme 35

NP_001031298.1

ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).

NP_001319409.1

ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 10164 Blast hits to 10157 proteins in 398 species: Archae - 0; Bacteria - 2; Metazoa - 4429; Fungi - 2211; Plants - 1825; Viruses - 23; Other Eukaryotes - 1674 (source: NCBI BLink).

NP_565192.1

ubiquitin-conjugating enzyme 35 (UBC35); FUNCTIONS IN: ubiquitin-protein ligase activity, protein binding; INVOLVED IN: root epidermal cell differentiation, response to cadmium ion, postreplication repair, response to iron ion, ubiquitin-dependent protein catabolic process; LOCATED IN: UBC13-MMS2 complex; EXPRESSED IN: male gametophyte, cultured cell, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Ubiquitin-conjugating enzyme/RWD-like (InterPro:IPR016135), Ubiquitin-conjugating enzyme, E2 (InterPro:IPR000608); BEST Arabidopsis thaliana protein match is: ubiquitin-conjugating enzyme 36 (TAIR:AT1G16890.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_003070.9 Reference assembly

Range

29650376..29652627

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_106535.4 → NP_565192.1 ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_565192.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q2V4C1, Q94A97, Q9ZVA6

UniProtKB/TrEMBL

A0A178WFI9, A0A5S9WVM9

Conserved Domains (1) summary

cl00154
Location:6 → 150

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...
NM_001334851.1 → NP_001321618.1 ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A1P8ARI0
NM_001334850.1 → NP_001319409.1 ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

Status: REVIEWED

UniProtKB/TrEMBL

A0A2H1ZEG2
NM_001036221.3 → NP_001031298.1 ubiquitin-conjugating enzyme 35 [Arabidopsis thaliana]

See identical proteins and their annotated locations for NP_001031298.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q94A97

Conserved Domains (2) summary

COG5078
Location:1 → 108

COG5078; Ubiquitin-protein ligase [Posttranslational modification, protein turnover, chaperones]

cl00154
Location:6 → 108

UBCc; Ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain. This is part of the ubiquitin-mediated protein degradation pathway in which a thiol-ester linkage forms between a conserved cysteine and the C-terminus of ubiquitin and complexes with ubiquitin ...