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SPB1 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase [ Saccharomyces cerevisiae S288C ]

Gene ID: 850304, updated on 28-Oct-2024

Summary

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Official Symbol
SPB1
Official Full Name
27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
Primary source
SGD:S000000559
Locus tag
YCL054W
See related
AllianceGenome:SGD:S000000559; FungiDB:YCL054W; VEuPathDB:YCL054W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables rRNA (guanine) methyltransferase activity and rRNA (uridine-2'-O-)-methyltransferase activity. Involved in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); and rRNA methylation. Located in nucleolus. Part of preribosome, large subunit precursor. Orthologous to human FTSJ3 (FtsJ RNA 2'-O-methyltransferase 3). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See SPB1 in Genome Data Viewer
Location:
chromosome: III
Exon count:
1
Sequence:
Chromosome: III; NC_001135.5 (31449..33974)

Chromosome III - NC_001135.5Genomic Context describing neighboring genes Neighboring gene Kar4p Neighboring gene Rdt1p Neighboring gene Pbn1p Neighboring gene Lre1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Spb1p is a putative methyltransferase required for 60S ribosomal subunit biogenesis in Saccharomyces cerevisiae. Kressler D, et al. Nucleic Acids Res, 1999 Dec 1. PMID 10556316, Free PMC Article
  2. Spb1p-directed formation of Gm2922 in the ribosome catalytic center occurs at a late processing stage. Lapeyre B, et al. Mol Cell, 2004 Nov 19. PMID 15546625
  3. Functional redundancy of Spb1p and a snR52-dependent mechanism for the 2'-O-ribose methylation of a conserved rRNA position in yeast. Bonnerot C, et al. Mol Cell, 2003 Nov. PMID 14636587
  4. Trm7p catalyses the formation of two 2'-O-methylriboses in yeast tRNA anticodon loop. Pintard L, et al. EMBO J, 2002 Apr 2. PMID 11927565, Free PMC Article
  5. The poly(A) binding protein is required for poly(A) shortening and 60S ribosomal subunit-dependent translation initiation. Sachs AB, et al. Cell, 1989 Sep 8. PMID 2673535

See all (84) citations in PubMed

GeneRIFs: Gene References Into Functions

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (guanine) methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (guanine) methyltransferase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables rRNA (guanosine-2'-O-)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (uridine-2'-O-)-methyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables rRNA (uridine-2'-O-)-methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
enables rRNA (uridine-2'-O-)-methyltransferase activity IGI
Inferred from Genetic Interaction
more info
 PubMed 
enables rRNA methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA 2'-O-methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA methylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in rRNA methylation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in rRNA methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in rRNA methylation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in rRNA methylation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA processing IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosome biogenesis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
is_active_in nucleolus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of preribosome, large subunit precursor IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of preribosome, large subunit precursor IDA
Inferred from Direct Assay
more info
 PubMed 
part_of preribosome, large subunit precursor IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
27S pre-rRNA (guanosine2922-2'-O)-methyltransferase
NP_009877.1
  • AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001135.5 Reference assembly

    Range
    31449..33974
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178698.1 → NP_009877.1  TPA: 27S pre-rRNA (guanosine2922-2'-O)-methyltransferase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009877.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQW3, P25582
    UniProtKB/TrEMBL
    A6ZTE1, C7GT08, G2W9X4, N1PAN5
    Conserved Domains (3) summary
    COG0293
    Location:1 → 206
    RlmE; 23S rRNA U2552 (ribose-2'-O)-methylase RlmE/FtsJ [Translation, ribosomal structure and biogenesis]
    pfam07780
    Location:621 → 835
    Spb1_C; Spb1 C-terminal domain
    pfam11861
    Location:236 → 383
    DUF3381; Domain of unknown function (DUF3381)
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P25582.2

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