STE2 alpha-factor pheromone receptor STE2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: STE2
Official Full Name: alpha-factor pheromone receptor STE2
Primary source: SGD:S000001868
Locus tag: YFL026W
See related: AllianceGenome:SGD:S000001868; FungiDB:YFL026W; VEuPathDB:YFL026W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables mating-type alpha-factor pheromone receptor activity. Involved in cytogamy and pheromone-dependent signal transduction involved in conjugation with cellular fusion. Located in plasma membrane. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See STE2 in Genome Data Viewer

Location:: chromosome: VI

Exon count:: 1

Sequence:: Chromosome: VI; NC_001138.5 (82580..83875)

Chromosome VI - NC_001138.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001138.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast alpha-Factor Receptor.
Title: Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast α-Factor Receptor.
Activation mechanism of the class D fungal GPCR dimer Ste2.
Title: Activation mechanism of the class D fungal GPCR dimer Ste2.
Structure of the class D GPCR Ste2 dimer coupled to two G proteins.
Title: Structure of the class D GPCR Ste2 dimer coupled to two G proteins.
Interaction between Ste2p and Gpa1p takes palce before ligand binding via the Ste2p C-terminal domain and the Gpa1p N-terminal domain.
Title: GPCR-Gα protein precoupling: Interaction between Ste2p, a yeast GPCR, and Gpa1p, its Gα protein, is formed before ligand binding via the Ste2p C-terminal domain and the Gpa1p N-terminal domain.
yeast pheromone receptor Ste2 forms predominantly tetramers at average expression levels of 2 to 25 molecules per pixel and a mixture of tetramers and octamers at expression levels of 25-100 molecules per pixel. Ste2 is a class D GPCR found in the yeast Saccharomyces cerevisiae of the mating type a, and binds the pheromone a-factor secreted secreted by cells of the mating type a
Title: Quaternary structure of the yeast pheromone receptor Ste2 in living cells.
The main difference between the Ste2 and Ste3 receptor observed in this work was that while hyperactive mutations were found for Ste3, no gain-of-function mutant for the Ste2 receptor was found. this fi nding is an outcome of the different strategies the two genes have adopted for their expression.
Title: Analysis of random PCR-originated mutants of the yeast Ste2 and Ste3 receptors.
Results suggest that the G-protein-coupled receptor, Ste2 binds the inhibitory G-protein signaling, Sst2 forming a complex with antagonistic functions.
Title: Yeast GPCR signaling reflects the fraction of occupied receptors, not the number.
Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast
Title: Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast.
results extend previous observations and quantify the contributions of Ste2p variants to mediating cell cycle arrest versus downstream mating functionalities.
Title: Quantification of mutation-derived bias for alternate mating functionalities of the Saccharomyces cerevisiae Ste2p pheromone receptor.
we propose that the N-terminus of Ste2p plays multiple regulatory roles in controlling receptor function.
Title: The N-terminus of the yeast G protein-coupled receptor Ste2p plays critical roles in surface expression, signaling, and negative regulation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables G protein-coupled receptor activity	IEA Inferred from Electronic Annotation more info
enables mating-type alpha-factor pheromone receptor activity	IDA Inferred from Direct Assay more info	PubMed
enables mating-type alpha-factor pheromone receptor activity	IGI Inferred from Genetic Interaction more info	PubMed
enables mating-type alpha-factor pheromone receptor activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mating-type factor pheromone receptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables mating-type factor pheromone receptor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in G protein-coupled receptor signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in cytogamy	IDA Inferred from Direct Assay more info	PubMed
involved_in cytogamy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in response to pheromone	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of G protein-coupled receptor homodimeric complex	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: alpha-factor pheromone receptor STE2

NP_116627.2

Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells