GRC3 polynucleotide 5'-hydroxyl-kinase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GRC3
Official Full Name: polynucleotide 5'-hydroxyl-kinase
Primary source: SGD:S000003958
Locus tag: YLL035W
See related: AllianceGenome:SGD:S000003958; FungiDB:YLL035W; VEuPathDB:YLL035W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables polynucleotide 5'-hydroxyl-kinase activity. Involved in cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) and termination of RNA polymerase I transcription. Located in nucleolar chromatin. Orthologous to human NOL9 (nucleolar protein 9). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See GRC3 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (68580..70478)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Identification of specific residues required to support Grc3-directed Las1-mediated pre-rRNA cleavage.
Title: Characterization of the molecular crosstalk within the essential Grc3/Las1 pre-rRNA processing complex.
Grc3 drives Las1 endoribonuclease cleavage to its targeted C2 site both in vitro and in Saccharomyces cerevisiae. The tetrameric Grc3/Las1 complex is a unique example of a protein-guided programmable endoribonuclease.
Title: Grc3 programs the essential endoribonuclease Las1 for specific RNA cleavage.
Authors propose that Las1 and Grc3 function together in a conserved mechanism to modulate rRNA processing and eukaryotic ribosome biogenesis.
Title: Las1 interacts with Grc3 polynucleotide kinase and is required for ribosome synthesis in Saccharomyces cerevisiae.
Grc3 is a polynucleotide kinase; inactivation reduces the efficiency of termination by Pol I.
Title: Role of the RNA/DNA kinase Grc3 in transcription termination by RNA polymerase I.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables polynucleotide 5'-hydroxyl-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables polynucleotide 5'-hydroxyl-kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables polynucleotide 5'-hydroxyl-kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IBA Inferred from Biological aspect of Ancestor more info
involved_in cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IDA Inferred from Direct Assay more info	PubMed
involved_in cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphorylation	IEA Inferred from Electronic Annotation more info
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in rRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in termination of RNA polymerase I transcription	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in nucleolar chromatin	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: polynucleotide 5'-hydroxyl-kinase

NP_013065.1

Polynucleotide kinase present on rDNA; required for efficient transcription termination by RNA polymerase I; functions with Las1p in a conserved mechanism to cleave pre-rRNA at a specific site during rRNA processing and ribosome biogenesis; required for cell growth; mRNA is cell-cycle regulated

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

68580..70478

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181855.1 → NP_013065.1 TPA: polynucleotide 5'-hydroxyl-kinase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013065.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXX0, Q07845, Q9P4W6

UniProtKB/TrEMBL

B3LTD7, N1NYD8

Conserved Domains (1) summary

COG1341
Location:163 → 610

Grc3; Polynucleotide 5'-kinase, involved in rRNA processing [Translation, ribosomal structure and biogenesis]