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REA1 AAA family ATPase midasin [ Saccharomyces cerevisiae S288C ]

Gene ID: 850796, updated on 18-Sep-2024

Summary

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Official Symbol
REA1
Official Full Name
AAA family ATPase midasin
Primary source
SGD:S000004096
Locus tag
YLR106C
See related
AllianceGenome:SGD:S000004096; FungiDB:YLR106C; VEuPathDB:YLR106C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
MDN1
Summary
Enables ATP hydrolysis activity. Involved in rRNA processing; regulation of ribosomal subunit export from nucleus; and ribonucleoprotein complex subunit organization. Located in nucleoplasm. Orthologous to human MDN1 (midasin AAA ATPase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See REA1 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (349006..363738, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene tRNA splicing endonuclease subunit SEN2 Neighboring gene ncRNA Neighboring gene RNA exonuclease Neighboring gene ncRNA

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1. Mickolajczyk KJ, et al. Proc Natl Acad Sci U S A, 2020 Aug 4. PMID 32694211, Free PMC Article
  2. The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1. Sosnowski P, et al. Elife, 2018 Nov 26. PMID 30460895, Free PMC Article
  3. Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. Hocine S, et al. Nat Methods, 2013 Feb. PMID 23263691, Free PMC Article
  4. Expression and genomic analysis of midasin, a novel and highly conserved AAA protein distantly related to dynein. Garbarino JE, et al. BMC Genomics, 2002 Jul 8. PMID 12102729, Free PMC Article
  5. Real-time observation of transcription initiation and elongation on an endogenous yeast gene. Larson DR, et al. Science, 2011 Apr 22. PMID 21512033, Free PMC Article

See all (97) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1.
    Title: Long-range intramolecular allostery and regulation in the dynein-like AAA protein Mdn1.
  2. The CryoEM molecular structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1 has been presented.
    Title: The CryoEM structure of the Saccharomyces cerevisiae ribosome maturation factor Rea1.
  3. Release of Nug2 from pre-60S particles requires both its K(+)-dependent GTPase activity and the remodelling ATPase activity of Rea1.
    Title: Coupled GTPase and remodelling ATPase activities form a checkpoint for ribosome export.
  4. Data suggest that Rea1 functions at successive maturation steps to remove ribosomal factors at critical transition points, first driving the exit of early pre-60S particles from the nucleolus and then driving late pre-60S particles from the nucleus.
    Title: The AAA-ATPase Rea1 drives removal of biogenesis factors during multiple stages of 60S ribosome assembly.
  5. Rea1 aligns with its interacting partners on the preribosome to effect a necessary step on the path to the export-competent 60S subunit.
    Title: Mechanochemical removal of ribosome biogenesis factors from nascent 60S ribosomal subunits.
  6. Rea1 is a dynein-related nuclear AAA-ATPase is involved in late rRNA processing and nuclear export of 60 S subunits
    Title: Rea1, a dynein-related nuclear AAA-ATPase, is involved in late rRNA processing and nuclear export of 60 S subunits.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Process Evidence Code Pubs
involved_in protein-RNA complex remodeling IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein-RNA complex remodeling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rRNA processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of ribosomal subunit export from nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosomal large subunit assembly IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in ribosomal large subunit assembly IEA
Inferred from Electronic Annotation
more info
  
involved_in ribosomal large subunit assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in ribosomal large subunit export from nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
Component Evidence Code Pubs
located_in mitochondrion HDA PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleoplasm IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleoplasm IEA
Inferred from Electronic Annotation
more info
  
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of preribosome, large subunit precursor IBA
Inferred from Biological aspect of Ancestor
more info
  

General protein information

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Preferred Names
AAA family ATPase midasin
NP_013207.1
  • Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex; involved with interaction partners Rsa4p and Ytm1p, in the ATP-dependent remodeling of the pre-60S particle at successive maturation steps during ribosomal biogenesis; involved in the removal of biogenesis factors including GTPase Nog2p prior to nuclear export; contains a hexameric AAA-motor head domain and a long flexible tail with a MIDAS (metal ion-dependent adhesion site) domain

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    349006..363738 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001181993.1 → NP_013207.1  TPA: AAA family ATPase midasin [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013207.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYA6, Q12019, Q7LGX0
    UniProtKB/TrEMBL
    N1NY63
    Conserved Domains (1) summary
    COG5271
    Location:89 → 4910
    MDN1; Midasin, AAA ATPase with vWA domain, involved in ribosome maturation [Translation, ribosomal structure and biogenesis]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q12019.1

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