DPH6 diphthine--ammonia ligase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DPH6
Official Full Name: diphthine--ammonia ligase
Primary source: SGD:S000004133
Locus tag: YLR143W
See related: AllianceGenome:SGD:S000004133; FungiDB:YLR143W; VEuPathDB:YLR143W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables diphthine-ammonia ligase activity. Involved in peptidyl-diphthamide biosynthetic process from peptidyl-histidine. Located in cytoplasm. Orthologous to human DPH6 (diphthamine biosynthesis 6). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See DPH6 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (427329..429386)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Through identification of Dph6 and Dph7 as components required for the amidation step of the diphthamide pathway, our work paves the way for a detailed mechanistic understanding of diphthamide formation.
Title: The amidation step of diphthamide biosynthesis in yeast requires DPH6, a gene identified through mining the DPH1-DPH5 interaction network.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables diphthine-ammonia ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables diphthine-ammonia ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables diphthine-ammonia ligase activity	IEA Inferred from Electronic Annotation more info
enables diphthine-ammonia ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ligase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in protein histidyl modification to diphthamide	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein histidyl modification to diphthamide	IDA Inferred from Direct Assay more info	PubMed
involved_in protein histidyl modification to diphthamide	IEA Inferred from Electronic Annotation more info
involved_in protein histidyl modification to diphthamide	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: diphthine--ammonia ligase

NP_013244.1

EC 6.3.1.14

Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

427329..429386

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182030.1 → NP_013244.1 TPA: diphthine--ammonia ligase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013244.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYD7, Q07261, Q12429

UniProtKB/TrEMBL

A7A141, B3LTA3, C7GMJ4, C8ZDB2, G2WIY1, N1P181

Conserved Domains (3) summary

cd06155
Location:313 → 417

eu_AANH_C_1; A group of hypothetical eukaryotic proteins, characterized by the presence of an adenine nucleotide alpha hydrolase (AANH)-like domain located N-terminal to two distinctly different YjgF-YER057c-UK114-like domains. This CD contains the first of these ...

cd06156
Location:449 → 571

eu_AANH_C_2; A group of hypothetical eukaryotic proteins, characterized by the presence of an adenine nucleotide alpha hydrolase (AANH)-like domain located N-terminal to two distinctly different YjgF-YER057c-UK114-like domains. This CD contains the second of these ...

TIGR00290
Location:1 → 264

MJ0570_dom; MJ0570-related uncharacterized domain