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DPH5 diphthine synthase [ Saccharomyces cerevisiae S288C ]

Gene ID: 850869, updated on 2-Nov-2024

Summary

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Official Symbol
DPH5
Official Full Name
diphthine synthase
Primary source
SGD:S000004162
Locus tag
YLR172C
See related
AllianceGenome:SGD:S000004162; FungiDB:YLR172C; VEuPathDB:YLR172C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables diphthine methyl ester synthase activity. Involved in protein histidyl modification to diphthamide. Located in cytoplasm. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with short stature, prominent forehead, and feeding difficulties. Orthologous to human DPH5 (diphthamide biosynthesis 5). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See DPH5 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (501260..502162, complement)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Ups2p Neighboring gene Aps1p Neighboring gene Tag1p Neighboring gene isocitrate dehydrogenase (NADP(+)) IDP2

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Biosynthesis of diphthamide in Saccharomyces cerevisiae. Partial purification and characterization of a specific S-adenosylmethionine:elongation factor 2 methyltransferase. Chen JY, et al. J Biol Chem, 1988 Aug 25. PMID 3042777
  2. Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis. Lin Z, et al. J Am Chem Soc, 2014 Apr 30. PMID 24739148, Free PMC Article
  3. QM/MM Studies of Dph5 - A Promiscuous Methyltransferase in the Eukaryotic Biosynthetic Pathway of Diphthamide. Hörberg J, et al. J Chem Inf Model, 2018 Jul 23. PMID 29927239
  4. A chemical genomic screen in Saccharomyces cerevisiae reveals a role for diphthamidation of translation elongation factor 2 in inhibition of protein synthesis by sordarin. Botet J, et al. Antimicrob Agents Chemother, 2008 May. PMID 18285480, Free PMC Article
  5. Diphtheria toxin-resistant mutants of Saccharomyces cerevisiae. Chen JY, et al. Mol Cell Biol, 1985 Dec. PMID 3915773, Free PMC Article

See all (46) citations in PubMed

GeneRIFs: Gene References Into Functions

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables diphthine methyl ester synthase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables diphthine methyl ester synthase activity IEA
Inferred from Electronic Annotation
more info
  
enables diphthine methyl ester synthase activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables methyltransferase activity IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in methylation IEA
Inferred from Electronic Annotation
more info
  
involved_in protein histidyl modification to diphthamide IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in protein histidyl modification to diphthamide IEA
Inferred from Electronic Annotation
more info
  
involved_in protein histidyl modification to diphthamide IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
diphthine synthase
NP_013273.1
  • Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    501260..502162 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182059.1 → NP_013273.1  TPA: diphthine synthase [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013273.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VYH6, P32469, Q6Q5L4
    UniProtKB/TrEMBL
    A7A163, B3RH65, B5VN90, C7GIW2, C8ZDE5, G2WJ05, N1P6Q7
    Conserved Domains (1) summary
    PTZ00175
    Location:1 → 257
    PTZ00175; diphthine synthase; Provisional
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P32469.1

Gene LinkOut

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