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CRN1 Crn1p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851148, updated on 2-Nov-2024

Summary

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Official Symbol
CRN1
Official Full Name
Crn1p
Primary source
SGD:S000004421
Locus tag
YLR429W
See related
AllianceGenome:SGD:S000004421; FungiDB:YLR429W; VEuPathDB:YLR429W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables Arp2/3 complex binding activity; cytoskeletal protein binding activity; and protein-macromolecule adaptor activity. Involved in several processes, including actin cortical patch localization; actin filament organization; and regulation of Arp2/3 complex-mediated actin nucleation. Located in actin cortical patch. Human ortholog(s) of this gene implicated in coronin-1A deficiency. Orthologous to several human genes including CORO6 (coronin 6). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See CRN1 in Genome Data Viewer
Location:
chromosome: XII
Exon count:
1
Sequence:
Chromosome: XII; NC_001144.5 (990777..992732)

Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Tda5p Neighboring gene Mag2p Neighboring gene DNA/RNA helicase SEN1 Neighboring gene Atg23p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The role of Saccharomyces cerevisiae coronin in the actin and microtubule cytoskeletons. Heil-Chapdelaine RA, et al. Curr Biol, 1998 Nov 19. PMID 9822583
  2. The intrinsically disordered region of coronins fine-tunes oligomerization and actin polymerization. Han X, et al. Cell Rep, 2023 Jun 27. PMID 37269287
  3. Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments. Ge P, et al. Nat Struct Mol Biol, 2014 Dec. PMID 25362487, Free PMC Article
  4. Overlapping and distinct functions for cofilin, coronin and Aip1 in actin dynamics in vivo. Lin MC, et al. J Cell Sci, 2010 Apr 15. PMID 20332110, Free PMC Article
  5. Coronin promotes the rapid assembly and cross-linking of actin filaments and may link the actin and microtubule cytoskeletons in yeast. Goode BL, et al. J Cell Biol, 1999 Jan 11. PMID 9885246, Free PMC Article

See all (75) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Using multi-color total internal reflection fluorescence microscopy, the study shows that Crn1 enhances Cof1-mediated severing by accelerating Cof1 binding to actin filament sides.
    Title: Coronin Enhances Actin Filament Severing by Recruiting Cofilin to Filament Sides and Altering F-Actin Conformation.
  2. The study reports cryo-EM structures for ADP-bound and ADP-beryllium fluoride (ADP-BeFx, an ADP-Pi mimic)-bound actin filaments in complex with the beta-propeller domain of yeast coronin 1 (crn1), at 8.6-A resolution.
    Title: Cryo-EM reveals different coronin binding modes for ADP- and ADP-BeFx actin filaments.
  3. development of a direct tethering recruitment model for activation of Arp2/3 complex by Crn1
    Title: Mechanism of a concentration-dependent switch between activation and inhibition of Arp2/3 complex by coronin.
  4. Conservation of the actin-binding surfaces across distant species and in all three major classes of coronin isoforms suggests that the nature of the coronin-actin association may be similar in other family members.
    Title: Functional surfaces on the actin-binding protein coronin revealed by systematic mutagenesis.
  5. Results suggest that that coronin polarizes the spatial distribution and activity of cofilin to promote selective disassembly of older actin filaments.
    Title: Coronin switches roles in actin disassembly depending on the nucleotide state of actin.
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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables Arp2/3 complex binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables actin binding IEA
Inferred from Electronic Annotation
more info
  
enables actin filament binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables actin filament binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables microtubule binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-macromolecule adaptor activity IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in actin cortical patch localization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament organization IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in actin filament organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in actin filament organization IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in microtubule-based process IPI
Inferred from Physical Interaction
more info
 PubMed 
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in negative regulation of Arp2/3 complex-mediated actin nucleation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of Arp2/3 complex-mediated actin nucleation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in actin cortical patch IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Crn1p
NP_013533.1
  • Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001144.5 Reference assembly

    Range
    990777..992732
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182317.1 → NP_013533.1  TPA: Crn1p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013533.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VZ64, Q06440
    UniProtKB/TrEMBL
    B3RHT8, N1NYV0
    Conserved Domains (4) summary
    sd00039
    Location:85 → 138
    7WD40; WD40 repeat [structural motif]
    NF033645
    Location:428 → 600
    pilus_FilE; putative pilus assembly protein FilE
    NF038014
    Location:521 → 641
    Chlamy_inclu_1; inclusion-associated protein
    cl29593
    Location:9 → 424
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q06440.1

Gene LinkOut

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