BRE1 E3 ubiquitin-protein ligase BRE1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: BRE1
Official Full Name: E3 ubiquitin-protein ligase BRE1
Primary source: SGD:S000002232
Locus tag: YDL074C
See related: AllianceGenome:SGD:S000002232; FungiDB:YDL074C; VEuPathDB:YDL074C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA replication origin binding activity; scaffold protein binding activity; and ubiquitin protein ligase activity. Involved in several processes, including DNA metabolic process; mitotic DNA damage checkpoint signaling; and subtelomeric heterochromatin formation. Located in chromatin. Is active in nucleus. Human ortholog(s) of this gene implicated in breast cancer. Orthologous to several human genes including RNF40 (ring finger protein 40). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See BRE1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (324047..326149, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.
Title: Bre1/RNF20 promotes Rad51-mediated strand exchange and antagonizes the Srs2/FBH1 helicases.
Structural basis for the Rad6 activation by the Bre1 N-terminal domain.
Title: Structural basis for the Rad6 activation by the Bre1 N-terminal domain.
Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex.
Title: Structure and functional determinants of Rad6-Bre1 subunits in the histone H2B ubiquitin-conjugating complex.
RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
Title: RPA-mediated recruitment of Bre1 couples histone H2B ubiquitination to DNA replication and repair.
Bre1 mediated-uH2B promotes DSB repair through facilitating histone eviction and subsequent loading of repair proteins
Title: Bre1-dependent H2B ubiquitination promotes homologous recombination by stimulating histone eviction at DNA breaks.
Bre1 mediates the ubiquitination of histone H2B by regulating Lge1 stability
Title: Bre1 mediates the ubiquitination of histone H2B by regulating Lge1 stability.
Bre1's function in G1 and S phases contributes to chromatid cohesion establishment, but it is not required for cohesion maintenance in G2 phase.
Title: E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication in Saccharomyces cerevisiae.
By docking onto the NCP surface in this distinct orientation, Bre1 positions the Rad6 active site directly over H2B Lys123. The Spt-Ada-Gcn5 acetyltransferase (SAGA) H2B deubiquitinase module competes with Bre1 for binding to the NCP acidic patch, indicating regulatory control. Our study reveals a mechanism that ensures site-specific NCP ubiquitination and fine-tuning of opposing enzymatic activities
Title: Structural mechanism for the recognition and ubiquitination of a single nucleosome residue by Rad6-Bre1.
Rad6-Bre1 pathway regulates both telomere length and recombination through its role in histone H2B lysine 123 mono-ubiquitination
Title: Rad6-Bre1-mediated H2B ubiquitination regulates telomere replication by promoting telomere-end resection.
Results identified Rai1, in regulating early transcription elongation genome-wide and a novel role for the histone modifier, Bre1, in regulating Pol II dynamics at polyA sites.
Title: Subgenic Pol II interactomes identify region-specific transcription elongation regulators.

Submit: New GeneRIF Correction See all GeneRIFs (19)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables scaffold protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin protein ligase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in double-strand break repair via homologous recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in meiotic DNA double-strand break formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic G1 DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info
involved_in regulation of DNA-templated DNA replication initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance via recombination	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription by RNA polymerase II	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
part_of HULC complex	IBA Inferred from Biological aspect of Ancestor more info
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
is_active_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: E3 ubiquitin-protein ligase BRE1

NP_010209.1

EC 2.3.2.27

E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

324047..326149 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180133.1 → NP_010209.1 TPA: E3 ubiquitin-protein ligase BRE1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010209.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRS5, Q07457

UniProtKB/TrEMBL

C8Z4J6, N1PA99

Conserved Domains (4) summary

COG1196
Location:314 → 600

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd16499
Location:646 → 687

RING-HC_BRE1_like; RING finger, HC subclass, found in yeast Bre1 and its homologs from eukaryotes

pfam08647
Location:441 → 535

BRE1; BRE1 E3 ubiquitin ligase

NF033930
Location:147 → 416

pneumo_PspA; pneumococcal surface protein A