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FOB1 replication fork barrier binding protein FOB1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 851688, updated on 2-Nov-2024

Summary

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Official Symbol
FOB1
Official Full Name
replication fork barrier binding protein FOB1
Primary source
SGD:S000002517
Locus tag
YDR110W
See related
AllianceGenome:SGD:S000002517; FungiDB:YDR110W; VEuPathDB:YDR110W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
HRM1
Summary
Enables rDNA spacer replication fork barrier binding activity. Involved in several processes, including DNA recombination; nucleolus organization; and regulation of DNA metabolic process. Located in nucleolus and rDNA heterochromatin. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See FOB1 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (676102..677802)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Trs85p Neighboring gene putative phosphotransferase Neighboring gene alanine transaminase ALT2 Neighboring gene securin

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Kobayashi T, et al. Genes Cells, 1996 May. PMID 9078378
  2. Quantification of the dynamic behaviour of ribosomal DNA genes and nucleolus during yeast Saccharomyces cerevisiae cell cycle. Dauban L, et al. J Struct Biol, 2019 Nov 1. PMID 31449968
  3. FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the Saccharomyces cerevisiae ribosomal DNA locus. Di Felice F, et al. Nucleic Acids Res, 2005. PMID 16269824, Free PMC Article
  4. The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Kobayashi T. Mol Cell Biol, 2003 Dec. PMID 14645529, Free PMC Article
  5. Behead and live long or the tale of cathepsin L. Streubel MK, et al. Yeast, 2018 Feb. PMID 29044689, Free PMC Article

See all (173) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.
    Title: Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.
  2. Smc5/6 in the rDNA modulates lifespan independently of Fob1.
    Title: Smc5/6 in the rDNA modulates lifespan independently of Fob1​.
  3. Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance
    Title: Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance.
  4. We conclude that Fob1 and Pol I make independent contributions to establishment of silencing, though Pol I also reinforces Fob1-dependent silencing.
    Title: RNA Polymerase I and Fob1 contributions to transcriptional silencing at the yeast rDNA locus.
  5. the replication terminator protein Fob1 of Saccharomyces cerevisiae promoted chromosome kissing that initiated rDNA recombination and controlled the replicative life span (RLS).
    Title: Mechanism of regulation of 'chromosome kissing' induced by Fob1 and its physiological significance.
  6. deletion of FOB1 corrects the genome-wide replication defects, nucleolar structure, and rDNA segregation that occur in a eco1 mutant that has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation.
    Title: The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.
  7. Replication fork arrest and rDNA silencing are two independent and separable functions of the replication terminator protein Fob1 of Saccharomyces cerevisiae.
    Title: Replication fork arrest and rDNA silencing are two independent and separable functions of the replication terminator protein Fob1 of Saccharomyces cerevisiae.
  8. Data show that viability of mother cells in liquid culture is regulated by SIR2 and FOB1, two opposing regulators of RLS in yeast.
    Title: The mother enrichment program: a genetic system for facile replicative life span analysis in Saccharomyces cerevisiae.
  9. Checkpoint proteins regulated the initiation and progression of recombination at Fob1-Ter (Ter sites are replication barrier sites) complexes.
    Title: Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrest.
  10. The binding to DNA of Fob1p itself may be the cause of the DNA topoisomerase I activity in the rDNA enhancer.
    Title: FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the Saccharomyces cerevisiae ribosomal DNA locus.
Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables rDNA spacer replication fork barrier binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables rDNA spacer replication fork barrier binding IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in chromatin organization IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in chromosome segregation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in maintenance of DNA repeat elements IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in maintenance of rDNA IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in negative regulation of DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of DNA recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein localization to nucleolar rDNA repeats IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rDNA chromatin condensation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in rDNA heterochromatin formation IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in rDNA heterochromatin formation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in replication fork arrest at rDNA repeats IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in rDNA heterochromatin IDA
Inferred from Direct Assay
more info
 PubMed 

General protein information

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Preferred Names
replication fork barrier binding protein FOB1
NP_010395.1
  • Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking and recombinational hotspot activity that results in concerted evolution of the rDNA, termed homogenization; role in recruitment of condensin to the replication fork barrier (RFB), contributing to the faithful segregation of rDNA repeats; proposed role in recovery from low rDNA copy number; related to retroviral integrases

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    676102..677802
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180418.1 → NP_010395.1  TPA: replication fork barrier binding protein FOB1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010395.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VS96, O13329, Q04587
    UniProtKB/TrEMBL
    B3LGG5, B5VG08, C7GIL9, C8Z526, G2WAL3, N1PAD5
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
O13329.2

Gene LinkOut

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