TFB1 TFIIH/NER complex subunit TFB1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: TFB1
Official Full Name: TFIIH/NER complex subunit TFB1
Primary source: SGD:S000002719
Locus tag: YDR311W
See related: AllianceGenome:SGD:S000002719; FungiDB:YDR311W; VEuPathDB:YDR311W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphatidylinositol-3-phosphate binding activity and phosphatidylinositol-5-phosphate binding activity. Involved in nucleic acid metabolic process and phosphorylation of RNA polymerase II C-terminal domain. Located in cytosol and nucleus. Part of nucleotide-excision repair factor 3 complex; transcription factor TFIIH core complex; and transcription factor TFIIH holo complex. Orthologous to human GTF2H1 (general transcription factor IIH subunit 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TFB1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1085065..1086993)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Interplay of the Tfb1 pleckstrin homology domain with Rad2 and Rad4 in transcription coupled and global genomic nucleotide excision repair.
Title: Interplay of the Tfb1 pleckstrin homology domain with Rad2 and Rad4 in transcription coupled and global genomic nucleotide excision repair.
DNA opening is catalyzed by Ssl2, the dsDNA translocase subunit of the basal transcription factor TFIIH. ATP hydrolysis by Ssl2 opens a 6-base-pair (bp) bubble that grows to 13 bp in the presence of NTPs. These observations support a two-step model wherein ATP-dependent Ssl2 translocation leads to a 6-bp open complex that RNA polymerase II expands via NTP-dependent RNA transcription.
Title: TFIIH generates a six-base-pair open complex during RNAP II transcription initiation and start-site scanning.
Structure determination of a Rad2-Tfb1PH complex indicates that Rad2 binds to TFIIH using a similar motif as TFIIEalpha uses to bind TFIIH in the pre-initiation complex.
Title: Structural and functional characterization of interactions involving the Tfb1 subunit of TFIIH and the NER factor Rad2.
NMR structure of the amino-terminal domain of the Tfb1 subunit
Title: 1H, 15N, and 13C resonance assignment of the amino-terminal domain of the Tfb1 subunit of yeast TFIIH.
The pleckstrin homology domain (residues 1-115) from the TFB1 subunit of TFIIH is sufficient for binding to the activation domain of herpes simplex virus protein VP16.
Title: NMR structure of the amino-terminal domain from the Tfb1 subunit of TFIIH and characterization of its phosphoinositide and VP16 binding sites.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol-3-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-5-phosphate binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IBA Inferred from Biological aspect of Ancestor more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IEA Inferred from Electronic Annotation more info
involved_in nucleotide-excision repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase I	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
part_of nucleotide-excision repair factor 3 complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIH core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIH core complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIH core complex	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIH holo complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIH holo complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: TFIIH/NER complex subunit TFB1

NP_010597.3

Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1085065..1086993

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180619.3 → NP_010597.3 TPA: TFIIH/NER complex subunit TFB1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010597.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VSU0, E9P948, P32776

UniProtKB/TrEMBL

A6ZYQ9, B3LFY5, B5VGJ1, C7GQW1, C8Z5M2, G2WB49, N1P6V8

Conserved Domains (3) summary

smart00751
Location:245 → 295

BSD; domain in transcription factors and synapse-associated proteins

pfam03909
Location:177 → 225

BSD; BSD domain

pfam08567
Location:9 → 105

PH_TFIIH; TFIIH p62 subunit, N-terminal domain