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DAD4 Dad4p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851919, updated on 28-Oct-2024

Summary

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Official Symbol
DAD4
Official Full Name
Dad4p
Primary source
SGD:S000007604
Locus tag
YDR320C-A
See related
AllianceGenome:SGD:S000007604; FungiDB:YDR320C-A; VEuPathDB:YDR320C-A
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
HSK2
Summary
Contributes to microtubule plus-end binding activity. Involved in attachment of spindle microtubules to kinetochore and positive regulation of microtubule polymerization. Part of DASH complex. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See DAD4 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (1108280..1108498, complement)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Yft2p Neighboring gene Swa2p Neighboring gene asparaginase ASP1 Neighboring gene mitochondrial 54S ribosomal protein YmL35

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. A method for reverse interactome analysis: High-resolution mapping of interdomain interaction network in Dam1 complex and its specific disorganization based on the interaction domain expression. Ikeuchi A, et al. Biotechnol Prog, 2010 Jul-Aug. PMID 20730753
  2. The Dam1 kinetochore complex harnesses microtubule dynamics to produce force and movement. Asbury CL, et al. Proc Natl Acad Sci U S A, 2006 Jun 27. PMID 16777964, Free PMC Article
  3. Protein arms in the kinetochore-microtubule interface of the yeast DASH complex. Miranda JJ, et al. Mol Biol Cell, 2007 Jul. PMID 17460120, Free PMC Article
  4. Phosphoregulation and depolymerization-driven movement of the Dam1 complex do not require ring formation. Gestaut DR, et al. Nat Cell Biol, 2008 Apr. PMID 18364702, Free PMC Article
  5. Ab initio reconstruction of helical samples with heterogeneity, disorder and coexisting symmetries. Ramey VH, et al. J Struct Biol, 2009 Aug. PMID 19447181, Free PMC Article

See all (43) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Results describe two novel subunits of DASH, Hsk2 and Hsk3 (helper of Ask1), and suggest that Hsk3 acts to incorporate Ask1 into the DASH complex.
    Title: Genetic analysis of the kinetochore DASH complex reveals an antagonistic relationship with the ras/protein kinase A pathway and a novel subunit required for Ask1 association.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
contributes_to microtubule binding IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to microtubule plus-end binding IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in attachment of spindle microtubules to kinetochore IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in attachment of spindle microtubules to kinetochore IEA
Inferred from Electronic Annotation
more info
  
involved_in cell division IEA
Inferred from Electronic Annotation
more info
  
involved_in chromosome segregation IEA
Inferred from Electronic Annotation
more info
  
involved_in positive regulation of microtubule polymerization IDA
Inferred from Direct Assay
more info
 PubMed 
Component Evidence Code Pubs
part_of DASH complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of DASH complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of DASH complex IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome IEA
Inferred from Electronic Annotation
more info
  
located_in chromosome, centromeric region IEA
Inferred from Electronic Annotation
more info
  
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytoskeleton IEA
Inferred from Electronic Annotation
more info
  
located_in kinetochore IEA
Inferred from Electronic Annotation
more info
  
located_in microtubule IEA
Inferred from Electronic Annotation
more info
  
located_in mitotic spindle IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in spindle IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Dad4p
NP_710144.3
  • Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    1108280..1108498 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001184470.3 → NP_710144.3  TPA: Dad4p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_710144.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VSV2, P69851
    UniProtKB/TrEMBL
    A6ZYS2, B3LFX7, C7GWL8, C8Z5N2, G2WB59, N1P590
    Conserved Domains (1) summary
    pfam08650
    Location:1 → 71
    DASH_Dad4; DASH complex subunit Dad4
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P69851.1

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