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MUS81 Mus81p [ Saccharomyces cerevisiae S288C ]

Gene ID: 851994, updated on 29-Jun-2024

Summary

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Gene symbol
MUS81
Gene description
Mus81p
Primary source
FungiDB:YDR386W
Locus tag
YDR386W
See related
SGD:S000002794; AllianceGenome:SGD:S000002794; VEuPathDB:YDR386W
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
SLX3
Summary
Enables enzyme inhibitor activity. Contributes to crossover junction endodeoxyribonuclease activity. Involved in DNA metabolic process and negative regulation of helicase activity. Located in nucleus. Part of Holliday junction resolvase complex. Orthologous to human MUS81 (MUS81 structure-specific endonuclease subunit). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See MUS81 in Genome Data Viewer
Location:
chromosome: IV
Exon count:
1
Sequence:
Chromosome: IV; NC_001136.10 (1246084..1247982)

Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene putative ammonium permease ATO3 Neighboring gene elongation factor 2 Neighboring gene Cin10p Neighboring gene amphiphysin

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. The possible function of Flp1 in homologous recombination repair in Saccharomyces cerevisiae. Phung HTT, et al. AIMS Genet, 2018. PMID 31435519, Free PMC Article
  2. Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage. Saugar I, et al. Cell Rep, 2017 Aug 15. PMID 28813668
  3. The Mus81-Mms4 structure-selective endonuclease requires nicked DNA junctions to undergo conformational changes and bend its DNA substrates for cleavage. Mukherjee S, et al. Nucleic Acids Res, 2014 Jun. PMID 24744239, Free PMC Article
  4. A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4. Ehmsen KT, et al. Nucleic Acids Res, 2009 Apr. PMID 19211663, Free PMC Article
  5. Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease. Ehmsen KT, et al. Nucleic Acids Res, 2008 Apr. PMID 18281703, Free PMC Article

See all (167) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.
    Title: MUS81 cleaves TOP1-derived lesions and other DNA-protein cross-links.
  2. The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
    Title: The cruciform DNA-binding protein Crp1 stimulates the endonuclease activity of Mus81-Mms4 in Saccharomyces cerevisiae.
  3. Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
    Title: Mus81-Mms4 endonuclease is an Esc2-STUbL-Cullin8 mitotic substrate impacting on genome integrity.
  4. Study presents evidence that yeast Mus81-Mms4 endonuclease contributes to cell viability in the absence of functional telomerase. This involvement is not through recombination-mediated telomere lengthening as proposed in humans, but rather through an indirect role involving replication stress. These and other results suggest that Mus81 localization to the telomere may be a promising indicator for telomere dysfunction.
    Title: Saccharomyces cerevisiae Mus81-Mms4 prevents accelerated senescence in telomerase-deficient cells.
  5. These results strongly support the notion that Rad52 and Mus81-Mms4 collaborate and work jointly in processing of homologous recombination intermediates.
    Title: Saccharomyces cerevisiae Mus81-Mms4 and Rad52 can cooperate in the resolution of recombination intermediates.
  6. Identification of new interacting partners and modulators of Mus81-Mms4 nuclease, RFC, and PCNA imply the cooperation of these factors in resolution of stalled replication forks and branched DNA structures emanating from the restarted replication forks under conditions of replication stress.
    Title: Role of PCNA and RFC in promoting Mus81-complex activity.
  7. The data suggest that subnuclear relocalization is relevant for the function of Mus81-Mms4 complex in response to DNA damage and, probably, of the endonucleases that colocalize with it.
    Title: Subnuclear Relocalization of Structure-Specific Endonucleases in Response to DNA Damage.
  8. The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation.
    Title: Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis.
  9. The data point to the possibility that the involvement of Esc2 in the metabolism of sister chromatid junctions is, in part, via modulation of the activity of the Mus81 complex.
    Title: Esc2 promotes Mus81 complex-activity via its SUMO-like and DNA binding domains.
  10. Our data suggest that Srs2 and Mus81-Mms4 have critical roles in preventing the formation of (or in resolving) toxic inter-homolog joint molecules, which could otherwise interfere with chromosome segregation and lead to genetic instability.
    Title: Srs2 and Mus81-Mms4 Prevent Accumulation of Toxic Inter-Homolog Recombination Intermediates.
Submit: New GeneRIF Correction See all GeneRIFs (31)

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables 3'-flap endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
contributes_to DNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
contributes_to crossover junction DNA endonuclease activity IBA
Inferred from Biological aspect of Ancestor
more info
  
contributes_to crossover junction DNA endonuclease activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables crossover junction DNA endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables endonuclease activity IEA
Inferred from Electronic Annotation
more info
  
enables enzyme inhibitor activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
Process Evidence Code Pubs
involved_in DNA catabolic process IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA damage response IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA topological change IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in double-strand break repair IEA
Inferred from Electronic Annotation
more info
  
involved_in double-strand break repair via break-induced replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in double-strand break repair via break-induced replication IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in double-strand break repair via break-induced replication IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in meiotic cell cycle IEA
Inferred from Electronic Annotation
more info
  
involved_in mitotic intra-S DNA damage checkpoint signaling IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in reciprocal meiotic recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in resolution of meiotic recombination intermediates IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in resolution of meiotic recombination intermediates IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of Holliday junction resolvase complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of Holliday junction resolvase complex IDA
Inferred from Direct Assay
more info
 PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IMP
Inferred from Mutant Phenotype
more info
 PubMed 

General protein information

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Preferred Names
Mus81p
NP_010674.3
  • Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA and DNA-protein cross-links; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates function of Mms4p-Mus81p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001136.10 Reference assembly

    Range
    1246084..1247982
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180694.3 → NP_010674.3  TPA: Mus81p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_010674.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VT20, Q04149
    UniProtKB/TrEMBL
    A0A8H8UNT3, A6ZYY7, B3LFR4, C7GSD6, C8Z5V0, N1P8S9
    Conserved Domains (2) summary
    COG1948
    Location:314 → 601
    MUS81; ERCC4-type nuclease [Replication, recombination and repair]
    cd21036
    Location:132 → 224
    WH_MUS81; winged helix domain found in crossover junction endonuclease MUS81 and similar proteins
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q04149.1

Gene LinkOut

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