RPB7 DNA-directed RNA polymerase II subunit RPB7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RPB7
Official Full Name: DNA-directed RNA polymerase II subunit RPB7
Primary source: SGD:S000002812
Locus tag: YDR404C
See related: AllianceGenome:SGD:S000002812; FungiDB:YDR404C; VEuPathDB:YDR404C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables single-stranded DNA binding activity; single-stranded RNA binding activity; and translation initiation factor binding activity. Contributes to RNA-dependent RNA polymerase activity. Involved in regulation of gene expression and transcription initiation at RNA polymerase II promoter. Located in P-body and nucleus. Part of RNA polymerase II, core complex. Used to study cancer. Orthologous to human POLR2G (RNA polymerase II subunit G). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RPB7 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1276654..1277169, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rpb7 represses transcription-coupled nucleotide excision repair.
Title: Rpb7 represses transcription-coupled nucleotide excision repair.
Rpb4/7, a key element of RNA pol II to coordinate mRNA synthesis in the nucleus with cytoplasmic functions in Saccharomyces cerevisiae.
Title: Rpb4/7, a key element of RNA pol II to coordinate mRNA synthesis in the nucleus with cytoplasmic functions in Saccharomyces cerevisiae.
Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.
Title: Structural visualization of de novo transcription initiation by Saccharomyces cerevisiae RNA polymerase II.
Numerous Post-translational Modifications of RNA Polymerase II Subunit Rpb4/7 Link Transcription to Post-transcriptional Mechanisms.
Title: Numerous Post-translational Modifications of RNA Polymerase II Subunit Rpb4/7 Link Transcription to Post-transcriptional Mechanisms.
The results show that the Rpb4/7 heterodimer in Saccharomyces cerevisiae plays a key role in controlling phosphorylation of the carboxy terminal domain of the Rpb1 subunit of RNA polymerase II.
Title: Rpb4/7 facilitates RNA polymerase II CTD dephosphorylation.
The interplay between Ccr4-Not and Rpb4/7 described here suggests a mechanism for how the cell coordinates mRNA synthesis and decay
Title: The Rpb4/7 module of RNA polymerase II is required for carbon catabolite repressor protein 4-negative on TATA (Ccr4-not) complex to promote elongation.
Rpb4 and Rpb7 subunits dissociate from RNA polymerase II upon interaction with specific transcriptional elongation-associated proteins that are recruited to the hyperphosphorylated form of the C-terminal RNA polymerase II domain.
Title: Quantitative proteomics demonstrates that the RNA polymerase II subunits Rpb4 and Rpb7 dissociate during transcriptional elongation.
growing RNA is leading toward Rpb4, not Rpb3, supporting the notion that RNA exits through the proposed pol II RNA exit channel 1
Title: Mapping RNA exit channel on transcribing RNA polymerase II by FRET analysis.
the complete Pol II associates with DNA in vivo, consistent with functional roles of Rpb4/7 throughout the transcription cycle.
Title: Genome-associated RNA polymerase II includes the dissociable Rpb4/7 subcomplex.
RPB4/RPB7 is a nucleo-cytoplasmic shuttling heterodimer that interacts with Pol II and with mRNAs and is required for mRNA decay in the cytoplasm.
Title: Transcription in the nucleus and mRNA decay in the cytoplasm are coupled processes.

Submit: New GeneRIF Correction See all GeneRIFs (15)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to RNA-dependent RNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables single-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded DNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables single-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables single-stranded RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables single-stranded RNA binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables translation initiation factor binding	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated transcription initiation	IEA Inferred from Electronic Annotation more info
involved_in RNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in nuclear-transcribed mRNA catabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in nuclear-transcribed mRNA catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of nuclear-transcribed mRNA poly(A) tail shortening	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of translational initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in transcription initiation at RNA polymerase II promoter	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription initiation at RNA polymerase II promoter	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
is_active_in P-body	IBA Inferred from Biological aspect of Ancestor more info
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IEA Inferred from Electronic Annotation more info
part_of RNA polymerase II, core complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RNA polymerase II, core complex	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
part_of nuclear DNA-directed RNA polymerase complex	IEA Inferred from Electronic Annotation more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DNA-directed RNA polymerase II subunit RPB7

NP_010692.3

RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1276654..1277169 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180712.3 → NP_010692.3 TPA: DNA-directed RNA polymerase II subunit RPB7 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010692.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VT36, P34087

UniProtKB/TrEMBL

A6ZZ03, B3LFP8, C7GSC0, C8Z5W7, G2WBE2, N1P5I1

Conserved Domains (1) summary

COG1095
Location:1 → 171

RPB7; DNA-directed RNA polymerase, subunit E'/Rpb7 [Transcription]