HEK2 Hek2p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: HEK2
Official Full Name: Hek2p
Primary source: SGD:S000000128
Locus tag: YBL032W
See related: AllianceGenome:SGD:S000000128; FungiDB:YBL032W; VEuPathDB:YBL032W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: KHD1
Summary: Enables mRNA binding activity. Involved in intracellular mRNA localization; mRNA stabilization; and telomere maintenance via telomerase. Located in P-body and chromosome, telomeric region. Orthologous to human PCBP3 (poly(rC) binding protein 3) and PCBP4 (poly(rC) binding protein 4). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See HEK2 in Genome Data Viewer

Location:: chromosome: II

Exon count:: 1

Sequence:: Chromosome: II; NC_001134.8 (160184..161329)

Chromosome II - NC_001134.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results suggest interdependence of Khd1p and mRNA for their targeting to the ER and presents additional evidence for signal sequence-independent, RBP-mediated mRNA targeting.
Title: Signal sequence-independent targeting of MID2 mRNA to the endoplasmic reticulum by the yeast RNA-binding protein Khd1p.
Mutations or physiological perturbations altering nuclear pore integrity decrease the levels of the nuclear pore complex (NPC)-associated SUMO protease Ulp1 and trigger the accumulation of sumoylated versions of Hek2 unable to bind NPC mRNAs. Study results support the existence of a quality control mechanism involving Ulp1 as a sensor of NPC integrity and Hek2 as a repressor of NPC biogenesis in budding yeast.
Title: A SUMO-dependent feedback loop senses and controls the biogenesis of nuclear pore subunits.
Khd1 and Ccr4 modulate a signal from Rho1 in the cell wall by regulating the expression of RhoGEF and RhoGAP.
Title: RNA-binding protein Khd1 and Ccr4 deadenylase play overlapping roles in the cell wall integrity pathway in Saccharomyces cerevisiae.
results suggest that MTL1 mRNA bears a cis-acting element involved in destabilization by the decapping enzyme and the 5'-3' exonuclease, and Khd1p stabilizes MTL1 mRNA through binding to this element
Title: Stability control of MTL1 mRNA by the RNA-binding protein Khd1p in yeast.
Khd1p differentially affects gene expression possibly due to combinatorial arrangement with additional factors reflecting the redundant structure of post-transcriptional regulatory systems.
Title: Distinct roles for Khd1p in the localization and expression of bud-localized mRNAs in yeast.
Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast.
Title: Epistatic interaction between the K-homology domain protein HEK2 and SIR1 at HMR and telomeres in yeast.
casein kinase Yck1p phosphorylates Khd1p at the plasma membrane, disrupting the Khd1p-RNA complex and releasing its translational repression on the ASH1 mRNA.
Title: Local activation of yeast ASH1 mRNA translation through phosphorylation of Khd1p by the casein kinase Yck1p.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA 3'-UTR AU-rich region binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in 3'-UTR-mediated mRNA stabilization	IBA Inferred from Biological aspect of Ancestor more info
involved_in chromatin organization	IEA Inferred from Electronic Annotation more info
involved_in intracellular mRNA localization	IDA Inferred from Direct Assay more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA stabilization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mRNA stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in telomere maintenance	IGI Inferred from Genetic Interaction more info	PubMed
involved_in telomere maintenance via telomerase	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in P-body	IDA Inferred from Direct Assay more info	PubMed
located_in P-body	IEA Inferred from Electronic Annotation more info
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in chromosome, telomeric region	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Hek2p

NP_009521.1

RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K; increases mRNA stability by binding (CNN)n repeat elements

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001134.8 Reference assembly

Range

160184..161329

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178272.1 → NP_009521.1 TPA: Hek2p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009521.1

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZKR5, D6VPW7, E9P8X5, P38199

UniProtKB/TrEMBL

G2W8X6, N1P8S7

Conserved Domains (3) summary

smart00322
Location:257 → 330

KH; K homology RNA-binding domain

cd22455
Location:44 → 113

KH-I_Rnc1_rpt1; first type I K homology (KH) RNA-binding domain found in Schizosaccharomyces pombe RNA-binding protein Rnc1 and similar proteins

cd22456
Location:158 → 226

KH-I_Rnc1_rpt2; second type I K homology (KH) RNA-binding domain found in Schizosaccharomyces pombe RNA-binding protein Rnc1 and similar proteins