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MED8 Med8p [ Saccharomyces cerevisiae S288C ]

Gene ID: 852492, updated on 28-Oct-2024

Summary

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Official Symbol
MED8
Official Full Name
Med8p
Primary source
SGD:S000000397
Locus tag
YBR193C
See related
AllianceGenome:SGD:S000000397; FungiDB:YBR193C; VEuPathDB:YBR193C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; TBP-class protein binding activity; and transcription corepressor activity. Involved in negative regulation of transcription by RNA polymerase II and positive regulation of transcription by RNA polymerase II. Part of core mediator complex. [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See MED8 in Genome Data Viewer
Location:
chromosome: II
Exon count:
1
Sequence:
Chromosome: II; NC_001134.8 (609082..609753, complement)

Chromosome II - NC_001134.8Genomic Context describing neighboring genes Neighboring gene ribosomal 60S subunit protein L21A Neighboring gene Rim2p Neighboring gene Aim4p Neighboring gene Msi1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001134.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Analysis by atomic force microscopy of Med8 binding to cis-acting regulatory elements of the SUC2 and HXK2 genes of saccharomyces cerevisiae. Moreno-Herrero F, et al. FEBS Lett, 1999 Oct 15. PMID 10526178
  2. Med8, a subunit of the mediator CTD complex of RNA polymerase II, directly binds to regulatory elements of SUC2 and HXK2 genes. Chaves RS, et al. Biochem Biophys Res Commun, 1999 Jan 19. PMID 9918841
  3. Mediator factor Med8p interacts with the hexokinase 2: implication in the glucose signalling pathway of Saccharomyces cerevisiae. de la Cera T, et al. J Mol Biol, 2002 Jun 7. PMID 12054864
  4. Rgt1, a glucose sensing transcription factor, is required for transcriptional repression of the HXK2 gene in Saccharomyces cerevisiae. Palomino A, et al. Biochem J, 2005 Jun 1. PMID 15705057, Free PMC Article
  5. Med8, Med18, and Med20 subunits of the Mediator head domain are interdependent upon each other for folding and complex formation. Adler D, et al. Proc Natl Acad Sci U S A, 2009 Dec 8. PMID 19934057, Free PMC Article

See all (88) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Deletion of med8C, med18, or med20 causes similar changes in the yeast transcriptome.
    Title: Structure-system correlation identifies a gene regulatory Mediator submodule.
  2. We identified Rgt1 as a transcription factor that, together with the Med8 protein, is essential for repression of the HXK2 gene in the absence of glucose.
    Title: Rgt1, a glucose sensing transcription factor, is required for transcriptional repression of the HXK2 gene in Saccharomyces cerevisiae.
  3. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.
    Title: Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables TBP-class protein binding IDA
Inferred from Direct Assay
more info
 PubMed 
enables protein-macromolecule adaptor activity IMP
Inferred from Mutant Phenotype
more info
 PubMed 
enables transcription coregulator activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables transcription coregulator activity IEA
Inferred from Electronic Annotation
more info
  
enables transcription corepressor activity IPI
Inferred from Physical Interaction
more info
 PubMed 
Process Evidence Code Pubs
involved_in negative regulation of transcription by RNA polymerase II IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in positive regulation of transcription by RNA polymerase II IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in regulation of transcription by RNA polymerase II IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in regulation of transcription by RNA polymerase II IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of core mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of core mediator complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of mediator complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of mediator complex IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Med8p
NP_009752.3
  • Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001134.8 Reference assembly

    Range
    609082..609753 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001178541.3 → NP_009752.3  TPA: Med8p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_009752.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VQI7, P38304
    UniProtKB/TrEMBL
    A6ZLD3, C7GKF2, D3UET7, G2W9J4, N1P9K0
    Conserved Domains (1) summary
    pfam10232
    Location:32 → 127
    Med8; Mediator of RNA polymerase II transcription complex subunit 8
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P38304.3

Gene LinkOut

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