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RPN14 Rpn14p [ Saccharomyces cerevisiae S288C ]

Gene ID: 852880, updated on 2-Nov-2024

Summary

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Official Symbol
RPN14
Official Full Name
Rpn14p
Primary source
SGD:S000002972
Locus tag
YGL004C
See related
AllianceGenome:SGD:S000002972; FungiDB:YGL004C; VEuPathDB:YGL004C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables protein folding chaperone. Involved in proteasome regulatory particle assembly and ubiquitin-dependent protein catabolic process. Located in cytosol and nucleus. Orthologous to human PAAF1 (proteasomal ATPase associated factor 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See RPN14 in Genome Data Viewer
Location:
chromosome: VII
Exon count:
1
Sequence:
Chromosome: VII; NC_001139.9 (490703..491956, complement)

Chromosome VII - NC_001139.9Genomic Context describing neighboring genes Neighboring gene calcium-transporting ATPase PMC1 Neighboring gene Golgi transport complex subunit COG7 Neighboring gene Cdh1p Neighboring gene Erp6p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Inhibition of 26S proteasome activity by α-synuclein is mediated by the proteasomal chaperone Rpn14/PAAF1. Galka D, et al. Aging Cell, 2024 May. PMID 38415292, Free PMC Article
  2. New crystal structure of the proteasome-dedicated chaperone Rpn14 at 1.6 Å resolution. Kim S, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2012 May 1. PMID 22691779, Free PMC Article
  3. Crystal structure of yeast rpn14, a chaperone of the 19 S regulatory particle of the proteasome. Kim S, et al. J Biol Chem, 2010 May 14. PMID 20236927, Free PMC Article
  4. Conformational dynamics of the Rpt6 ATPase in proteasome assembly and Rpn14 binding. Ehlinger A, et al. Structure, 2013 May 7. PMID 23562395, Free PMC Article
  5. An inducible chaperone adapts proteasome assembly to stress. Hanssum A, et al. Mol Cell, 2014 Aug 21. PMID 25042801, Free PMC Article

See all (51) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Rpn14 binds selectively to Rpt6's four-helix bundle, with surprisingly high affinity
    Title: Conformational dynamics of the Rpt6 ATPase in proteasome assembly and Rpn14 binding.
  2. crystal structure of Rpn14 with an E384A mutation is presented in space group P2(1) at 1.6 A resolution
    Title: New crystal structure of the proteasome-dedicated chaperone Rpn14 at 1.6 Å resolution.
  3. By inspection of structural data along with genetic and biochemical data, we determined the specific residues of Rpn14 and Rpt6 for complementary charge interactions that are required for 19 S RP assembly
    Title: Crystal structure of yeast rpn14, a chaperone of the 19 S regulatory particle of the proteasome.
  4. RPN14 protein involved in the efficient recognition of 26S proteasome for the proteolysis of ubiquitinated Gcn4p.
    Title: Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables protein folding chaperone IDA
Inferred from Direct Assay
more info
 PubMed 
Process Evidence Code Pubs
involved_in proteasome regulatory particle assembly IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in proteasome regulatory particle assembly IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein folding IEA
Inferred from Electronic Annotation
more info
  
involved_in ubiquitin-dependent protein catabolic process IMP
Inferred from Mutant Phenotype
more info
 PubMed 
Component Evidence Code Pubs
located_in cytoplasm HDA PubMed 
located_in cytoplasm IEA
Inferred from Electronic Annotation
more info
  
located_in cytosol IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of proteasome complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
Rpn14p
NP_011511.3
  • Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001139.9 Reference assembly

    Range
    490703..491956 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001180869.3 → NP_011511.3  TPA: Rpn14p [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_011511.3

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VUD4, P53196
    UniProtKB/TrEMBL
    G2WE90, N1P210
    Conserved Domains (2) summary
    sd00039
    Location:96 → 133
    7WD40; WD40 repeat [structural motif]
    cl29593
    Location:133 → 357
    WD40; WD40 domain, found in a number of eukaryotic proteins that cover a wide variety of functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly; typically contains a GH dipeptide 11-24 residues from ...
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P53196.1

Gene LinkOut

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