KSS1 mitogen-activated serine/threonine-protein kinase KSS1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: KSS1
Gene description: mitogen-activated serine/threonine-protein kinase KSS1
Primary source: FungiDB:YGR040W
Locus tag: YGR040W
See related: SGD:S000003272; AllianceGenome:SGD:S000003272; VEuPathDB:YGR040W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables MAP kinase activity. Involved in several processes, including invasive growth in response to glucose limitation; pheromone-dependent signal transduction involved in conjugation with cellular fusion; and signal transduction involved in filamentous growth. Located in cellular bud neck and nucleus. Human ortholog(s) of this gene implicated in Noonan syndrome 13; carcinoma (multiple); and pulmonary hypertension. Orthologous to several human genes including MAPK1 (mitogen-activated protein kinase 1). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See KSS1 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (575398..576504)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Our work highlights Fus3 and Kss1 as important early-stage regulators of the chronological lifespan of yeast
Title: Mitogen-activated protein kinases, Fus3 and Kss1, regulate chronological lifespan in yeast.
Transcriptional induction by Kss1 requires its hyperphosphorylation. Pheromone signaling reduces the negative effect of Kss1 while increasing the positive effect of Fus3. Negative feedback from Fus3 limits activation of Kss1.
Title: Analysis of the thresholds for transcriptional activation by the yeast MAP kinases Fus3 and Kss1.
We find that Kss1 also localizes to the sites of polarized growth and Bem3 interacts with Kss1 at the septin-ring assembly site. TD2 localization is largely mediated by its interaction with the polarisome component Epo1 via heterotypic coiled-coil interaction. This finding reveals a novel role for the polarisome in linking Bem3 to its functional target,Cdc42.
Title: Roles of an N-terminal coiled-coil-containing domain in the localization and function of Bem3, a Rho GTPase-activating protein in budding yeast.
Ahk1 is a scaffold protein in the HKR1 subbranch and prevents incorrect signal flow from Hkr1 to Kss1
Title: Scaffold Protein Ahk1, Which Associates with Hkr1, Sho1, Ste11, and Pbs2, Inhibits Cross Talk Signaling from the Hkr1 Osmosensor to the Kss1 Mitogen-Activated Protein Kinase.
the pea2-M409L mutation results in decreased invasive filamentation and elongation, diminished activity of a Kss1p MAPK pathway reporter, decreased unipolar budding, and diminished binding of the polarisome protein Spa2p.
Title: Pooled segregant sequencing reveals genetic determinants of yeast pseudohyphal growth.
Kss1p interacts physically with Cdc37p
Title: Cdc37p is involved in osmoadaptation and controls high osmolarity-induced cross-talk via the MAP kinase Kss1p.
Activation of the high-osmolarity glycerol pathway results in the phosphorylation of Kss1.
Title: Hog1 mitogen-activated protein kinase (MAPK) interrupts signal transduction between the Kss1 MAPK and the Tec1 transcription factor to maintain pathway specificity.
The reciprocal inhibitory loop between Kss1 and Hog1 allows only one or the other of these MAPKs to be stably activated under various stress conditions.
Title: Glycosylation defects activate filamentous growth Kss1 MAPK and inhibit osmoregulatory Hog1 MAPK.
Results examine the role of docking interactions in determining connectivity of the yeast MAPKs Fus3 and Kss1.
Title: The role of docking interactions in mediating signaling input, output, and discrimination in the yeast MAPK network.
KSS1 is activated by Ste4 and Ste5 and also by 1-butanol.
Title: Differential input by Ste5 scaffold and Msg5 phosphatase route a MAPK cascade to multiple outcomes.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

rep_origin

Loc: 575484-576346

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables MAP kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase activity	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	HDA more info	PubMed
enables protein kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in MAPK cascade	IEA Inferred from Electronic Annotation more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in invasive growth in response to glucose limitation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IBA Inferred from Biological aspect of Ancestor more info
involved_in pheromone-dependent signal transduction involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of division septum assembly	IGI Inferred from Genetic Interaction more info	PubMed
involved_in response to pheromone triggering conjugation with cellular fusion	IDA Inferred from Direct Assay more info	PubMed
involved_in signal transduction involved in filamentous growth	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in periplasmic space	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mitogen-activated serine/threonine-protein kinase KSS1

NP_011554.3

EC 2.7.11.24

Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in some S288C-related strains and functional in W303a strains

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

575398..576504

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181169.3 → NP_011554.3 TPA: mitogen-activated serine/threonine-protein kinase KSS1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011554.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VUH8, P14681, Q45U43

UniProtKB/TrEMBL

A0A8H4C0F3, A6ZV28, B3LII1, B5VIZ4, C7GXM6, C8Z8T6, G2WED0, N1P9U9

Conserved Domains (1) summary

cl21453
Location:7 → 365

PKc_like; Protein Kinases, catalytic domain