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Kars1 lysyl-tRNA synthetase 1 [ Mus musculus (house mouse) ]

Gene ID: 85305, updated on 2-Nov-2024

Summary

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Official Symbol
Kars1provided by MGI
Official Full Name
lysyl-tRNA synthetase 1provided by MGI
Primary source
MGI:MGI:1934754
See related
Ensembl:ENSMUSG00000031948 AllianceGenome:MGI:1934754
Gene type
protein coding
RefSeq status
VALIDATED
Organism
Mus musculus
Lineage
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as
Kars; LysRS; D8Wsu108e; mKIAA0070; D8Ertd698e
Summary
Enables scaffold protein binding activity. Predicted to be involved in several processes, including ERK1 and ERK2 cascade; diadenosine tetraphosphate biosynthetic process; and lysyl-tRNA aminoacylation. Located in mitochondrion. Part of aminoacyl-tRNA synthetase multienzyme complex. Is expressed in several structures, including alimentary system; central nervous system; cranium; genitourinary system; and sensory organ. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease recessive intermediate B and autosomal recessive nonsyndromic deafness 89. Orthologous to human KARS1 (lysyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Nov 2024]
Expression
Ubiquitous expression in CNS E11.5 (RPKM 43.4), placenta adult (RPKM 35.9) and 28 other tissues See more
Orthologs
human all
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Genomic context

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See Kars1 in Genome Data Viewer
Location:
8 E1; 8 58.27 cM
Exon count:
17
Annotation release Status Assembly Chr Location
RS_2024_02 current GRCm39 (GCF_000001635.27) 8 NC_000074.7 (112720071..112737986, complement)
108.20200622 previous assembly GRCm38.p6 (GCF_000001635.26) 8 NC_000074.6 (111993439..112011354, complement)

Chromosome 8 - NC_000074.7Genomic Context describing neighboring genes Neighboring gene STARR-seq mESC enhancer starr_22729 Neighboring gene GABA type A receptor associated protein like 2 Neighboring gene adenosine deaminase, tRNA-specific 1 Neighboring gene CapStarr-seq enhancer MGSCv37_chr8:114500469-114500652 Neighboring gene STARR-positive B cell enhancer ABC_E6687 Neighboring gene STARR-positive B cell enhancer ABC_E1376 Neighboring gene telomeric repeat binding factor 2, interacting protein Neighboring gene heterogeneous nuclear ribonucleoprotein A3 pseudogene Neighboring gene predicted gene 9564

Genomic regions, transcripts, and products

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Go to nucleotide: Graphics FASTA GenBank

Expression

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  • Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
  • Description: RNA profiling data sets generated by the Mouse ENCODE project.
  • BioProject: PRJNA66167
  • Publication: PMID 25409824
  • Analysis date: n/a

Bibliography

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Related articles in PubMed

  1. KARS Mutations Impair Brain Myelination by Inducing Oligodendrocyte Deficiency: One Potential Mechanism and Improvement by Melatonin. Yu L, et al. J Pineal Res, 2024 Aug. PMID 39087379
  2. Lysyl-Transfer RNA Synthetase Induces the Maturation of Dendritic Cells through MAPK and NF-κB Pathways, Strongly Contributing to Enhanced Th1 Cell Responses. Lim HX, et al. J Immunol, 2018 Nov 1. PMID 30275047
  3. Nonconventional involvement of LysRS in the molecular mechanism of USF2 transcriptional activity in FcepsilonRI-activated mast cells. Lee YN, et al. Mol Cell Biol, 2005 Oct. PMID 16199869, Free PMC Article
  4. Noncanonical roles of membranous lysyl-tRNA synthetase in transducing cell-substrate signaling for invasive dissemination of colon cancer spheroids in 3D collagen I gels. Nam SH, et al. Oncotarget, 2015 Aug 28. PMID 26091349, Free PMC Article
  5. Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis. Nam SH, et al. J Clin Invest, 2018 Nov 1. PMID 30188867, Free PMC Article

See all (47) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. KARS Mutations Impair Brain Myelination by Inducing Oligodendrocyte Deficiency: One Potential Mechanism and Improvement by Melatonin.
    Title: KARS Mutations Impair Brain Myelination by Inducing Oligodendrocyte Deficiency: One Potential Mechanism and Improvement by Melatonin.
  2. KRS in colon cancer cells remodels the microenvironment to promote metastasis, which can thus be therapeutically targeted at these bidirectional KRS-dependent communications of cancer spheroids with environmental cues.
    Title: Lysyl-tRNA synthetase-expressing colon spheroids induce M2 macrophage polarization to promote metastasis.
  3. these results indicated that KRS effectively induced the maturation and activation of dendritic cells
    Title: Lysyl-Transfer RNA Synthetase Induces the Maturation of Dendritic Cells through MAPK and NF-κB Pathways, Strongly Contributing to Enhanced Th1 Cell Responses.
  4. mitoKARS is the first described member of a group of mitochondrial proteins whose interaction with mutant SOD1 contributes to mitochondrial dysfunction in ALS
    Title: Lysyl-tRNA synthetase is a target for mutant SOD1 toxicity in mitochondria.
Submit: New GeneRIF Correction

Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics  (MGI)
  • Endonuclease-mediated (4) 
  • Gene trapped (1) 
  • Targeted (2) 

Pathways from PubChem

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Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Markers

Homology

Gene Ontology Provided by MGI

Function Evidence Code Pubs
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding ISO
Inferred from Sequence Orthology
more info
  
enables ATP:ADP adenylyltransferase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables ATP:ADP adenylyltransferase activity ISO
Inferred from Sequence Orthology
more info
  
enables ATP:ADP adenylyltransferase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables amino acid binding ISO
Inferred from Sequence Orthology
more info
  
enables identical protein binding ISO
Inferred from Sequence Orthology
more info
  
enables lysine-tRNA ligase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables lysine-tRNA ligase activity ISO
Inferred from Sequence Orthology
more info
  
enables lysine-tRNA ligase activity ISS
Inferred from Sequence or Structural Similarity
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables protein homodimerization activity ISO
Inferred from Sequence Orthology
more info
  
enables scaffold protein binding IPI
Inferred from Physical Interaction
more info
 PubMed 
enables tRNA binding IBA
Inferred from Biological aspect of Ancestor
more info
  
enables tRNA binding ISO
Inferred from Sequence Orthology
more info
  
Process Evidence Code Pubs
involved_in ERK1 and ERK2 cascade ISO
Inferred from Sequence Orthology
more info
  
involved_in basophil activation involved in immune response IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in basophil activation involved in immune response ISO
Inferred from Sequence Orthology
more info
  
involved_in diadenosine tetraphosphate biosynthetic process IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in diadenosine tetraphosphate biosynthetic process ISO
Inferred from Sequence Orthology
more info
  
involved_in diadenosine tetraphosphate biosynthetic process ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in lysyl-tRNA aminoacylation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in lysyl-tRNA aminoacylation ISO
Inferred from Sequence Orthology
more info
  
involved_in lysyl-tRNA aminoacylation ISS
Inferred from Sequence or Structural Similarity
more info
  
involved_in positive regulation of DNA-templated transcription ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of inflammatory response to antigenic stimulus ISO
Inferred from Sequence Orthology
more info
  
involved_in positive regulation of macrophage activation IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in positive regulation of macrophage activation ISO
Inferred from Sequence Orthology
more info
  
involved_in response to X-ray ISO
Inferred from Sequence Orthology
more info
  
Component Evidence Code Pubs
part_of aminoacyl-tRNA synthetase multienzyme complex IBA
Inferred from Biological aspect of Ancestor
more info
  
part_of aminoacyl-tRNA synthetase multienzyme complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of aminoacyl-tRNA synthetase multienzyme complex ISO
Inferred from Sequence Orthology
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in cytoplasm ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISO
Inferred from Sequence Orthology
more info
  
located_in cytosol ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in extracellular region IEA
Inferred from Electronic Annotation
more info
  
located_in extracellular space ISO
Inferred from Sequence Orthology
more info
  
located_in mitochondrion HDA PubMed 
is_active_in mitochondrion IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in mitochondrion ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISO
Inferred from Sequence Orthology
more info
  
located_in nucleus ISS
Inferred from Sequence or Structural Similarity
more info
  
located_in plasma membrane IEA
Inferred from Electronic Annotation
more info
  
located_in plasma membrane ISO
Inferred from Sequence Orthology
more info
  

General protein information

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Preferred Names
lysine--tRNA ligase
NP_001124340.1
NP_001273313.1
NP_001350358.1
NP_001350359.1
NP_444322.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

  1. NM_001130868.2 → NP_001124340.1  lysine--tRNA ligase isoform 1

    See identical proteins and their annotated locations for NP_001124340.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 4. Variants 1, 3, 4, and 5 all encode the same isoform (1).
    Source sequence(s)
    AC114648, AK167693, W77297
    Consensus CDS
    CCDS52677.1
    UniProtKB/TrEMBL
    Q8CFK5, Q8R2P8
    Related
    ENSMUSP00000090808.6, ENSMUST00000093120.13
    Conserved Domains (1) summary
    PLN02502
    Location:42 → 604
    PLN02502; lysyl-tRNA synthetase

  2. NM_001286384.1 → NP_001273313.1  lysine--tRNA ligase isoform 1

    See identical proteins and their annotated locations for NP_001273313.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 4. Variants 1, 3, 4, and 5 all encode the same isoform (1).
    Source sequence(s)
    AC114648, AK167693, W77297
    Consensus CDS
    CCDS52677.1
    UniProtKB/TrEMBL
    Q8CFK5, Q8R2P8
    Related
    ENSMUSP00000126268.2, ENSMUST00000164470.3
    Conserved Domains (1) summary
    PLN02502
    Location:42 → 604
    PLN02502; lysyl-tRNA synthetase

  3. NM_001363429.1 → NP_001350358.1  lysine--tRNA ligase isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (5) differs in the 5' UTR compared to variant 4. Variants 1, 3, 4, and 5 all encode the same isoform (1).
    Source sequence(s)
    AC114648
    Consensus CDS
    CCDS52677.1
    UniProtKB/TrEMBL
    Q8CFK5, Q8R2P8
    Conserved Domains (1) summary
    PLN02502
    Location:42 → 604
    PLN02502; lysyl-tRNA synthetase

  4. NM_001363430.1 → NP_001350359.1  lysine--tRNA ligase isoform 1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (4) represents the longest transcript and encodes the longer isoform (1). Variants 1, 3, 4, and 5 all encode the same isoform (1).
    Source sequence(s)
    AC114648
    Consensus CDS
    CCDS52677.1
    UniProtKB/TrEMBL
    Q8CFK5, Q8R2P8
    Conserved Domains (1) summary
    PLN02502
    Location:42 → 604
    PLN02502; lysyl-tRNA synthetase

  5. NM_053092.3 → NP_444322.1  lysine--tRNA ligase isoform 2

    See identical proteins and their annotated locations for NP_444322.1

    Status: VALIDATED

    Description
    Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 4. The resulting isoform (2) is shorter compared to isoform 1.
    Source sequence(s)
    AC114648, AK167693, W77297
    Consensus CDS
    CCDS40481.1
    UniProtKB/Swiss-Prot
    Q99MN1
    UniProtKB/TrEMBL
    Q3TIV6, Q8C292
    Related
    ENSMUSP00000034426.7, ENSMUST00000034426.14
    Conserved Domains (4) summary
    PLN02502
    Location:12 → 575
    PLN02502; lysyl-tRNA synthetase
    cd00775
    Location:235 → 573
    LysRS_core; Lys_tRNA synthetase (LysRS) class II core domain. Class II LysRS is a dimer which attaches a lysine to the 3' OH group of ribose of the appropriate tRNA. Its assignment to class II aaRS is based upon its structure and the presence of three ...
    cd04322
    Location:123 → 232
    LysRS_N; LysRS_N: N-terminal, anticodon recognition domain of lysyl-tRNA synthetases (LysRS). These enzymes are homodimeric class 2b aminoacyl-tRNA synthetases (aaRSs). This domain is a beta-barrel domain (OB fold) involved in binding the tRNA anticodon stem-loop. ...
    pfam08496
    Location:14 → 45
    Peptidase_S49_N; Peptidase family S49 N-terminal

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

  1. NC_000074.7 Reference GRCm39 C57BL/6J

    Range
    112720071..112737986 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)
Nucleotide Protein Strain
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
Q99MN1.1 GenPept UniProtKB/Swiss-Prot:Q99MN1

Gene LinkOut

The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.

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