NIT2 putative hydrolase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NIT2
Official Full Name: putative hydrolase
Primary source: SGD:S000003662
Locus tag: YJL126W
See related: AllianceGenome:SGD:S000003662; FungiDB:YJL126W; VEuPathDB:YJL126W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables deaminated glutathione amidase activity. Involved in amide catabolic process. Predicted to be located in mitochondrion. Orthologous to human NIT1 (nitrilase 1). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See NIT2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (184501..185424)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Nit2 crystal structure suggests that Nit2 may have broad biological roles in yeast, especially in regard to nitrogen homeostasis.
Title: Structures of enzyme-intermediate complexes of yeast Nit2: insights into its catalytic mechanism and different substrate specificity compared with mammalian Nit2.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables [acetyl-CoA carboxylase]-phosphatase activity	IEA Inferred from Electronic Annotation more info
enables deaminated glutathione amidase activity	IDA Inferred from Direct Assay more info	PubMed
enables deaminated glutathione amidase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in amide catabolic process	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cellular_component	ND No biological Data available more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: putative hydrolase

NP_012409.1

EC 3.5.1.128

Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

184501..185424

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181559.1 → NP_012409.1 TPA: putative hydrolase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012409.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW60, P47016, Q71SQ0

UniProtKB/TrEMBL

A6ZQK5, C7GQ03, C8ZB93

Conserved Domains (1) summary

cd07572
Location:7 → 302

nit; Nit1, Nit 2, and related proteins, and the Nit1-like domain of NitFhit (class 10 nitrilases)