EXO70 GTP-Rho binding exocyst subunit EXO70 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: EXO70
Official Full Name: GTP-Rho binding exocyst subunit EXO70
Primary source: SGD:S000003621
Locus tag: YJL085W
See related: AllianceGenome:SGD:S000003621; FungiDB:YJL085W; VEuPathDB:YJL085W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphatidylinositol-4,5-bisphosphate binding activity and small GTPase binding activity. Involved in several processes, including Golgi to plasma membrane transport; Rho protein signal transduction; and exocyst assembly. Located in cellular bud neck; cellular bud tip; and plasma membrane. Part of exocyst and incipient cellular bud site. Orthologous to human EXOC7 (exocyst complex component 7). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See EXO70 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (272827..274698)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Exocyst structural changes associated with activation of tethering downstream of Rho/Cdc42 GTPases.
Title: Exocyst structural changes associated with activation of tethering downstream of Rho/Cdc42 GTPases.
Blocking actin-dependent delivery of secretory vesicles in act1-3 cells results in loss of Sec3p from the purified complex. The results are consistent with a model in which Sec3p and Exo70p can function exclusively on the plasma membrane while the other subunits are brought to them on secretory vesicles.
Title: Two subunits of the exocyst, Sec3p and Exo70p, can function exclusively on the plasma membrane.
Data indicate structural details of the interaction of exocyst complex Sec3p and Exo70p with lipid bilayers containing phosphatidylinositol (4,5)-bisphosphate (PIP2) by molecular dynamics simulations.
Title: Membrane targeting of the yeast exocyst complex.
Exo70p directly and specifically binds to the polarity determinant scaffold protein Bem1p. The interaction involves multiple domains of both Exo70p and Bem1p.
Title: Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p.
Data show that Exo70 has the biochemical properties expected of a direct effector for both Rho3 and Cdc42, and suggest that interaction with the Exo70 component of the exocyst is a key event in spatial regulation of exocytosis by Rho GTPases.
Title: The Exo70 subunit of the exocyst is an effector for both Cdc42 and Rho3 function in polarized exocytosis.
The results suggest that either Exo70p or Sec3p must associate with the plasma membrane for the exocyst to function as a vesicle tether.
Title: An internal domain of Exo70p is required for actin-independent localization and mediates assembly of specific exocyst components.
Exo70 mediates the assembly of the exocyst complex at the plasma membrane, which is a crucial step in the tethering of post-Golgi secretory vesicles for exocytosis.
Title: Exo70 interacts with phospholipids and mediates the targeting of the exocyst to the plasma membrane.
structure provides a first view of the Exo70 protein family and provides a framework to study the molecular function of this exocyst component
Title: Crystal structure of the S.cerevisiae exocyst component Exo70p.
The structure of the exocyst subunit Exo70p is presented.
Title: The structures of exocyst subunit Exo70p and the Exo84p C-terminal domains reveal a common motif.
Exo70p functions primarily at early stages of the cell cycle in Bgl2p vesicle secretion, which is critical for polarized cell growth.
Title: Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables phosphatidylinositol-4,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4,5-bisphosphate binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	IDA Inferred from Direct Assay more info	PubMed
enables small GTPase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in Golgi to plasma membrane transport	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in Rho protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
involved_in Rho protein signal transduction	IGI Inferred from Genetic Interaction more info	PubMed
involved_in Rho protein signal transduction	IPI Inferred from Physical Interaction more info	PubMed
involved_in exocyst assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in exocyst localization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cellular bud	IEA Inferred from Electronic Annotation more info
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IEA Inferred from Electronic Annotation more info
located_in cellular bud tip	HDA more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasmic vesicle	IEA Inferred from Electronic Annotation more info
part_of exocyst	IBA Inferred from Biological aspect of Ancestor more info
part_of exocyst	IDA Inferred from Direct Assay more info	PubMed
part_of exocyst	IEA Inferred from Electronic Annotation more info
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed
located_in mating projection tip	HDA more info	PubMed
located_in plasma membrane	IDA Inferred from Direct Assay more info	PubMed
located_in prospore membrane	HDA more info	PubMed
located_in transport vesicle	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: GTP-Rho binding exocyst subunit EXO70

NP_012450.1

Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

272827..274698

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181518.1 → NP_012450.1 TPA: GTP-Rho binding exocyst subunit EXO70 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012450.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VW98, E9P961, P19658

UniProtKB/TrEMBL

A6ZPN9, B3LQ27, C7GR28, G2WGT2, N1P190

Conserved Domains (1) summary

pfam03081
Location:236 → 620

Exo70; Exo70 exocyst complex subunit