SMC3 cohesin subunit SMC3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SMC3
Official Full Name: cohesin subunit SMC3
Primary source: SGD:S000003610
Locus tag: YJL074C
See related: AllianceGenome:SGD:S000003610; FungiDB:YJL074C; VEuPathDB:YJL074C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein kinase binding activity. Contributes to ATP hydrolysis activity. Involved in nuclear division and replication-born double-strand break repair via sister chromatid exchange. Located in nucleus. Part of nuclear mitotic cohesin complex. Human ortholog(s) of this gene implicated in Cornelia de Lange syndrome 3. Orthologous to human SMC3 (structural maintenance of chromosomes 3). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SMC3 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (299157..302849, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
Title: Conformational dynamics of cohesin/Scc2 loading complex are regulated by Smc3 acetylation and ATP binding.
A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
Title: A model for Scc2p stimulation of cohesin's ATPase and its inhibition by acetylation of Smc3p.
DNA passes through cohesin's hinge as well as its Smc3-kleisin interface.
Title: DNA passes through cohesin's hinge as well as its Smc3-kleisin interface.
Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
Title: Smc3 acetylation, Pds5 and Scc2 control the translocase activity that establishes cohesin-dependent chromatin loops.
The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.
Title: The Interplay of Cohesin and the Replisome at Processive and Stressed DNA Replication Forks.
Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
Title: Functional control of Eco1 through the MCM complex in sister chromatid cohesion.
ATP-binding and Smc1-Smc3 heterodimerization promote conformational changes within the ATPase that are transmitted to the Smc coiled-coil domains.
Title: The structure of the cohesin ATPase elucidates the mechanism of SMC-kleisin ring opening.
The authors show here that Scc2 also actively maintains cohesin on chromosomes during G1 in S. cerevisiae cells. It does so by blocking a Wapl-independent release reaction that requires opening the cohesin ring at its Smc3/Scc1 interface as well as the D loop of Smc1's ATPase.
Title: Scc2 counteracts a Wapl-independent mechanism that releases cohesin from chromosomes during G1.
The X. laevis Eco2 bound to its primary and secondary Smc3 substrates demonstrate the plasticity of the substrate-binding site, which confers substrate specificity by concerted conformational changes of the central beta-hairpin and the C-terminal extension.
Title: Structural Basis of Eco1-Mediated Cohesin Acetylation.
Smc3 deacetylation by Hos1, triggered by Scc1 cleavage, facilitates efficient dissolution of sister chromatid cohesion during early metaphase.
Title: Smc3 Deacetylation by Hos1 Facilitates Efficient Dissolution of Sister Chromatid Cohesion during Early Anaphase.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
contributes_to ATP hydrolysis activity	IDA Inferred from Direct Assay more info	PubMed
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables double-stranded DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in G2/M transition of mitotic cell cycle	IPI Inferred from Physical Interaction more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome organization	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in meiotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in reciprocal meiotic recombination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in replication-born double-strand break repair via sister chromatid exchange	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in synaptonemal complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome	IEA Inferred from Electronic Annotation more info
part_of mitotic cohesin complex	IBA Inferred from Biological aspect of Ancestor more info
part_of mitotic cohesin complex	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: cohesin subunit SMC3

NP_012461.1

Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

299157..302849 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181507.1 → NP_012461.1 TPA: cohesin subunit SMC3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012461.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWA9, P47037

UniProtKB/TrEMBL

B3LQ37, N1P196

Conserved Domains (1) summary

COG1196
Location:1 → 1221

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]