SUI2 translation initiation factor eIF2 subunit alpha [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SUI2
Official Full Name: translation initiation factor eIF2 subunit alpha
Primary source: SGD:S000003767
Locus tag: YJR007W
See related: AllianceGenome:SGD:S000003767; FungiDB:YJR007W; VEuPathDB:YJR007W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to GTP binding activity and methionyl-initiator methionine tRNA binding activity. Involved in formation of translation preinitiation complex. Located in cytoplasmic stress granule. Part of eukaryotic 48S preinitiation complex; eukaryotic translation initiation factor 2 complex; and multi-eIF complex. Used to study leukodystrophy. Human ortholog(s) of this gene implicated in Alzheimer's disease. Orthologous to human EIF2S1 (eukaryotic translation initiation factor 2 subunit alpha). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See SUI2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (451020..451934)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eIF2alpha interactions with mRNA control accurate start codon selection by the translation preinitiation complex.
Title: eIF2α interactions with mRNA control accurate start codon selection by the translation preinitiation complex.
Data show that translation initiation factor 2alpha [eukaryotic translation initiation factor 2alpha (eIF2alpha; sui2) was phosphorylated at serine51 (Ser51) by of eIF2alpha kinase and downregulated cellular protein synthesis.
Title: Phosphorylation of translation initiation factor eIF2α at Ser51 depends on site- and context-specific information.
Study used single-particle electron cryomicroscopy to resolve the structures of phosphorylated (eIF2alphaP) and unphosphorylated (eIF2) from Saccharomyces cerevisiae bound with its nucleotide exchange factor eIF2B. The structures reveal that eIF2 undergoes large rearrangements to promote binding of eIF2alpha to the regulatory core of eIF2B comprised of the eIF2B alpha, beta and delta subunits.
Title: The structural basis of translational control by eIF2 phosphorylation.
These data uncover competition between eIF2B and eIF5 for eIF2-GTP/Met-tRNAi ternary complexes and identify that phosphorylated eIF2-GTP translation initiation intermediate complexes can be inhibited by eIF2B.
Title: Fail-safe control of translation initiation by dissociation of eIF2α phosphorylated ternary complexes.
Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2alpha phosphorylation.
Title: Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2α phosphorylation.
Data indicate that Cdc123 is a specific assembly factor for eukaryotic translation initiation factor 2 (eIF) subunit alpha (sui2), subunit beta (sui3) and subunit gamma (GCD11), which is indispensable for the onset of protein synthesis.
Title: Translation initiation requires cell division cycle 123 (Cdc123) to facilitate biogenesis of the eukaryotic initiation factor 2 (eIF2).
study found that phosphorylation of eIF2alpha by methylglyoxal occurred independently of the target of rapamycin (TOR) pathway
Title: Methylglyoxal activates Gcn2 to phosphorylate eIF2alpha independently of the TOR pathway in Saccharomyces cerevisiae.
Mutationally disrupting the hydrophobic network in various ways constitutively activates eIF2alpha phosphorylation in vivo and bypasses the requirement for a key tRNA binding motif (m2) and Thr-882 in Gcn2.
Title: A network of hydrophobic residues impeding helix alphaC rotation maintains latency of kinase Gcn2, which phosphorylates the alpha subunit of translation initiation factor 2.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
contributes_to GTP binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
contributes_to methionyl-initiator methionine tRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables ribosome binding	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IBA Inferred from Biological aspect of Ancestor more info
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in formation of translation preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in translational initiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytosol	IEA Inferred from Electronic Annotation more info
part_of eukaryotic 48S preinitiation complex	IBA Inferred from Biological aspect of Ancestor more info
part_of eukaryotic 48S preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic translation initiation factor 2 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of eukaryotic translation initiation factor 2 complex	IMP Inferred from Mutant Phenotype more info	PubMed
part_of multi-eIF complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF2 subunit alpha

NP_012540.3

Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

451020..451934

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181664.3 → NP_012540.3 TPA: translation initiation factor eIF2 subunit alpha [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012540.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWI2, E9P8T6, P20459

UniProtKB/TrEMBL

A6ZPX4, B3LQA6, C7GUT4, D3UF74, G2WH17, N1P3B9

Conserved Domains (1) summary

PTZ00248
Location:5 → 290

PTZ00248; eukaryotic translation initiation factor 2 subunit 1; Provisional