POL32 DNA polymerase delta subunit POL32 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: POL32
Official Full Name: DNA polymerase delta subunit POL32
Primary source: SGD:S000003804
Locus tag: YJR043C
See related: AllianceGenome:SGD:S000003804; FungiDB:YJR043C; VEuPathDB:YJR043C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: REV5
Summary: Enables DNA-directed DNA polymerase activity and H3-H4 histone complex chaperone activity. Involved in DNA metabolic process. Located in nucleus. Part of zeta DNA polymerase complex. Used to study cancer. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See POL32 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (516461..517513, complement)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

DNA polymerase delta governs parental histone transfer to DNA replication lagging strand.
Title: DNA polymerase delta governs parental histone transfer to DNA replication lagging strand.
Structure and mechanism of B-family DNA polymerase zeta specialized for translesion DNA synthesis.
Title: Structure and mechanism of B-family DNA polymerase ζ specialized for translesion DNA synthesis.
The structure reveals an unexpected arrangement in which the regulatory subunits (Pol31 and Pol32) lie next to the exonuclease domain of Pol3 but do not engage the DNA.
Title: Cryo-EM structure and dynamics of eukaryotic DNA polymerase δ holoenzyme.
PCNA enhanced the nucleotide incorporation rate by >10 fold. Compared to wild-type, a growth-defective yeast PCNA mutant (DD41,42AA) showed substantially less stimulation of the Pol delta nucleotide incorporation rate, identifying the face of PCNA that is important for the acceleration of catalysis.
Title: PCNA accelerates the nucleotide incorporation rate by DNA polymerase δ.
we observed a similar increase in repeat instability in RNase H-deficient cells when we either changed the direction of transcription-replication collisions, or flipped the repeat sequence such that the (UUC)n run occurred in the transcript. The increase in repeat expansions in RNase H-deficient strains was dependent on Rad52 and Pol32 proteins, suggesting that break-induced replication (BIR) is responsible for this effe
Title: RNA-DNA hybrids promote the expansion of Friedreich's ataxia (GAA)n repeats via break-induced replication.
Rev3 role in Pol32 regulation in the course of mutagenesis
Title: A novel variant of DNA polymerase ζ, Rev3ΔC, highlights differential regulation of Pol32 as a subunit of polymerase δ versus ζ in Saccharomyces cerevisiae.
Pol31 and Pol32 subunits of yeast DNA polymerase delta are also essential subunits of DNA polymerase zeta.
Title: Pol31 and Pol32 subunits of yeast DNA polymerase δ are also essential subunits of DNA polymerase ζ.
A rad3/XPD mutation causes TFIIH retention at DNA damage and reduces gap filling. This rad3/XPD mutation causes replication fork breakage and double strand breakes rescued by Rad51- or Pol32-dependent recombination.
Title: A postincision-deficient TFIIH causes replication fork breakage and uncovers alternative Rad51- or Pol32-mediated restart mechanisms.
propose an orientation for the accessory Pol31-Pol32N subunits relative to the Pol3 catalytic core that best agrees with the experimental scattering profile
Title: Structural insights into yeast DNA polymerase delta by small angle X-ray scattering.
Translocations rely on homologous recombination (HR) proteins, such as DNA repair enzymes, as well as on the break-induced replication-specific protein Pol32 and on Srs2.
Title: Chromosomal translocations caused by either pol32-dependent or pol32-independent triparental break-induced replication.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA-directed DNA polymerase activity	IDA Inferred from Direct Assay more info	PubMed
enables DNA-directed DNA polymerase activity	IEA Inferred from Electronic Annotation more info
contributes_to DNA-directed DNA polymerase activity	TAS Traceable Author Statement more info	PubMed
enables H3-H4 histone complex chaperone activity	IDA Inferred from Direct Assay more info	PubMed
enables H3-H4 histone complex chaperone activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to single-stranded DNA 3'-5' DNA exonuclease activity	TAS Traceable Author Statement more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA amplification	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication, removal of RNA primer	IDA Inferred from Direct Assay more info	PubMed
involved_in RNA-templated DNA biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in base-excision repair	TAS Traceable Author Statement more info	PubMed
involved_in double-strand break repair via break-induced replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in error-prone translesion synthesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in lagging strand elongation	TAS Traceable Author Statement more info	PubMed
involved_in leading strand elongation	TAS Traceable Author Statement more info	PubMed
involved_in nucleotide-excision repair	TAS Traceable Author Statement more info	PubMed

Component	Evidence Code	Pubs
part_of delta DNA polymerase complex	TAS Traceable Author Statement more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of zeta DNA polymerase complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: DNA polymerase delta subunit POL32

NP_012577.1

Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; parental H3-H4 histone chaperone that mediates transfer of parental histones to the lagging strand via Pol1p during DNA replication; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001142.9 Reference assembly

Range

516461..517513 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001181701.1 → NP_012577.1 TPA: DNA polymerase delta subunit POL32 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012577.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VWL4, P47110

UniProtKB/TrEMBL

G2WH47

Conserved Domains (1) summary

pfam09507
Location:125 → 348

CDC27; DNA polymerase subunit Cdc27