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APE1 metalloaminopeptidase APE1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 853758, updated on 2-Nov-2024

Summary

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Official Symbol
APE1
Official Full Name
metalloaminopeptidase APE1
Primary source
SGD:S000001586
Locus tag
YKL103C
See related
AllianceGenome:SGD:S000001586; FungiDB:YKL103C; VEuPathDB:YKL103C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as
API; LAP4; YSC1
Summary
Enables metalloaminopeptidase activity. Involved in cytoplasm to vacuole targeting by the Cvt pathway. Located in fungal-type vacuole. Part of Cvt complex. [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See APE1 in Genome Data Viewer
Location:
chromosome: XI
Exon count:
1
Sequence:
Chromosome: XI; NC_001143.9 (246138..247682, complement)

Chromosome XI - NC_001143.9Genomic Context describing neighboring genes Neighboring gene Seg2p Neighboring gene glutamine--fructose-6-phosphate transaminase (isomerizing) GFA1 Neighboring gene uncharacterized protein Neighboring gene uncharacterized protein Neighboring gene protein kinase HSL1

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Transport of a large oligomeric protein by the cytoplasm to vacuole protein targeting pathway. Kim J, et al. J Cell Biol, 1997 May 5. PMID 9151668, Free PMC Article
  2. Structure of yeast Ape1 and its role in autophagic vesicle formation. Su MY, et al. Autophagy, 2015. PMID 26208681, Free PMC Article
  3. Lap4, a vacuolar aminopeptidase I, is involved in cadmium-glutathione metabolism. Adamis PD, et al. Biometals, 2009 Apr. PMID 18716881
  4. Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway. Adachi W, et al. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2007 Mar 1. PMID 17329814, Free PMC Article
  5. The prepropeptide of vacuolar aminopeptidase I is necessary and sufficient to target the fluorescent reporter protein GFP to the vacuole of yeast by the Ccvt pathway. Martinez E, et al. Mol Microbiol, 1999 Jul. PMID 10411723

See all (119) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. APE1/Ref-1 Role in Inflammation and Immune Response.
    Title: APE1/Ref-1 Role in Inflammation and Immune Response.
  2. Ape1 aggregates bind Atg19 and Atg8 in vitro; this could be used as a scaffold for an in vitro assay of autophagosome formation to elucidate the mechanisms of autophagy
    Title: Propeptide of aminopeptidase 1 protein mediates aggregation and vesicle formation in cytoplasm-to-vacuole targeting pathway.
  3. under cadmium stress, Lap4 and gamma-glutamyl transferase seem to work together to assure an efficient glutathione turnover stored in the vacuole
    Title: Lap4, a vacuolar aminopeptidase I, is involved in cadmium-glutathione metabolism.
  4. Mature Ape1 (mApe1) has been expressed, purified and crystallized in two crystal forms. Forms I and II contain 12 and four mApe1 molecules per asymmetric unit, respectively, and mApe1 exists as a tetrahedral dodecamer in both crystal forms.
    Title: Crystallization of Saccharomyces cerevisiae aminopeptidase 1, the major cargo protein of the Cvt pathway.
Submit: New GeneRIF Correction

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables aminopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables metal ion binding IEA
Inferred from Electronic Annotation
more info
  
enables metalloaminopeptidase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables metalloaminopeptidase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables metallopeptidase activity IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in cytoplasm to vacuole targeting by the Cvt pathway IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in protein transport IEA
Inferred from Electronic Annotation
more info
  
involved_in proteolysis IEA
Inferred from Electronic Annotation
more info
  
Component Evidence Code Pubs
part_of Cvt complex IDA
Inferred from Direct Assay
more info
 PubMed 
located_in cytoplasm HDA PubMed 
is_active_in fungal-type vacuole IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in fungal-type vacuole IDA
Inferred from Direct Assay
more info
 PubMed 
located_in vacuole IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
metalloaminopeptidase APE1
NP_012819.1
  • Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to peptidase family M18; often used as marker in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via CVT pathway

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001143.9 Reference assembly

    Range
    246138..247682 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001179669.1 → NP_012819.1  TPA: metalloaminopeptidase APE1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_012819.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6VXI5, P14904, P22060
    UniProtKB/TrEMBL
    A6ZZL4, B3LQZ3, C7GVK8, C8ZC72, G2WHT1, N1NZ36
    Conserved Domains (1) summary
    cd05639
    Location:53 → 502
    M18; M18 peptidase aminopeptidase family
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P14904.2

Gene LinkOut

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