THI20 trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: THI20
Gene description: trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase
Primary source: FungiDB:YOL055C
Locus tag: YOL055C
See related: SGD:S000005416; AllianceGenome:SGD:S000005416; VEuPathDB:YOL055C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables hydroxymethylpyrimidine kinase activity; phosphomethylpyrimidine kinase activity; and thiaminase activity. Involved in thiamine biosynthetic process and thiamine catabolic process. Located in cytosol. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See THI20 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (224420..226075, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The TenA domain of THI20 possesses some features of both classes, consistent with its ability to hydrolyze both thiamin and the thiamin-degradation product 2-methyl-4-amino-5-aminomethylpyrimidine.
Title: Structure of trifunctional THI20 from yeast.
It is proposed that the thiaminase II activity of Thi20p is involved in the thiamin salvage pathway by catalyzing the hydrolysis of HMP precursors in S. cerevisiae.
Title: Involvement of thiaminase II encoded by the THI20 gene in thiamin salvage of Saccharomyces cerevisiae.
Thi20 is a trifunctional protein with thiamin biosynthetic and degradative activity
Title: Thi20, a remarkable enzyme from Saccharomyces cerevisiae with dual thiamin biosynthetic and degradation activities.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables hydroxymethylpyrimidine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables hydroxymethylpyrimidine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables hydroxymethylpyrimidine kinase activity	IEA Inferred from Electronic Annotation more info
enables hydroxymethylpyrimidine kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphomethylpyrimidine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables phosphomethylpyrimidine kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables phosphomethylpyrimidine kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphomethylpyrimidine kinase activity	IGI Inferred from Genetic Interaction more info	PubMed
enables thiaminase activity	IDA Inferred from Direct Assay more info	PubMed
enables thiaminase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in thiamine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in thiamine biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in thiamine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in thiamine catabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in thiamine diphosphate biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase

NP_014586.1

EC 2.7.1.49

EC 2.7.4.7

EC 3.5.99.2

Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

224420..226075 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183310.1 → NP_014586.1 TPA: trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014586.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W212, Q05664, Q08224, Q6B2F0

UniProtKB/TrEMBL

A0A6A5Q6K6, A6ZNF5, B3LJ04, B5VRJ6, C7GK22, C8ZHQ8, G2WMH9, N1NWB8

Conserved Domains (2) summary

TIGR04306
Location:343 → 550

salvage_TenA; thiaminase II

COG0351
Location:22 → 296

ThiD; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase [Coenzyme transport and metabolism]