TIM44 protein translocase subunit TIM44 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: TIM44
Gene description: protein translocase subunit TIM44
Primary source: FungiDB:YIL022W
Locus tag: YIL022W
See related: SGD:S000001284; AllianceGenome:SGD:S000001284; VEuPathDB:YIL022W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: ISP45; MIM44; MPI1
Summary: Enables chaperone binding activity and protein-macromolecule adaptor activity. Involved in protein import into mitochondrial matrix. Located in mitochondrion. Part of PAM complex, Tim23 associated import motor. Used to study thyroid gland Hurthle cell carcinoma. Orthologous to human TIMM44 (translocase of inner mitochondrial membrane 44). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See TIM44 in Genome Data Viewer

Location:: chromosome: IX

Exon count:: 1

Sequence:: Chromosome: IX; NC_001141.2 (311165..312460)

Chromosome IX - NC_001141.2 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001141.2

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Structural basis of mitochondrial protein import by the TIM23 complex.
Title: Structural basis of mitochondrial protein import by the TIM23 complex.
Using site-specific in vivo crosslinking and genetic approaches in Saccharomyces cerevisiae, the authors found that both domains of Tim44 interact with the major matrix-exposed loop of Tim23, with the C-terminal domain (CTD) binding Tim17 as well.
Title: Dual interaction of scaffold protein Tim44 of mitochondrial import motor with channel-forming translocase subunit Tim23.
These data suggest that the translocation channel and the import motor of the TIM23 complex communicate through rearrangements of the two domains of Tim44 that are stimulated by translocating proteins.
Title: Protein translocation channel of mitochondrial inner membrane and matrix-exposed import motor communicate via two-domain coupling protein.
Tim23 mitochondrial motor components are tethered to translocon via the scaffold Tim44, with regulatory protein Pam17 having independent interaction site.
Title: Architecture of the TIM23 inner mitochondrial translocon and interactions with the matrix import motor.
Genetic and biochemical analyses reveal synthetic interactions between PAM17 and TIM44.
Title: Pam17 and Tim44 act sequentially in protein import into the mitochondrial matrix.
Tim44 is not simply a scaffold for binding of motor subunits but plays a differential role in the recruitment of PAM modules to the inner membrane translocase.
Title: Mitochondrial protein import motor: differential role of Tim44 in the recruitment of Pam17 and J-complex to the presequence translocase.
The N-terminal 167-amino-acid segment of mature Tim44 is sufficient for both interaction with mtHsp70 and destabilization of a Tim44-mtHsp70 complex caused by client protein binding.
Title: Residues of Tim44 involved in both association with the translocon of the inner mitochondrial membrane and regulation of mitochondrial Hsp70 tethering.
binding of Tim44 to mHsp70 prevents the formation of a complex between the latter and Tim14/Pam18-Tim16/Pam16.
Title: The interplay between components of the mitochondrial protein translocation motor studied using purified components.
interactions between Tim44 and mtHsp70, controlled by polypeptide binding, are required for efficient translocation across the mitochondrial inner membrane
Title: Regulated interactions of mtHsp70 with Tim44 at the translocon in the mitochondrial inner membrane.
To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, yeast Tim44p has been crystallized.
Title: Preliminary crystallographic studies of yeast mitochondrial peripheral membrane protein Tim44p.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables protein-folding chaperone binding	IBA Inferred from Biological aspect of Ancestor more info
enables protein-folding chaperone binding	IEA Inferred from Electronic Annotation more info
enables protein-folding chaperone binding	IMP Inferred from Mutant Phenotype more info	PubMed
enables protein-folding chaperone binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in protein import into mitochondrial matrix	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein import into mitochondrial matrix	IEA Inferred from Electronic Annotation more info
involved_in protein import into mitochondrial matrix	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of PAM complex, Tim23 associated import motor	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrial inner membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial inner membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein translocase subunit TIM44

NP_012242.1

Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001141.2 Reference assembly

Range

311165..312460

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179372.1 → NP_012242.1 TPA: protein translocase subunit TIM44 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012242.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VVQ7, Q01852

UniProtKB/TrEMBL

A0A6A5Q2Y5, A6ZVP5, B3LTN0, B5VKR9, C7GPX0, G2WGC2, N1P463

Conserved Domains (1) summary

TIGR00984
Location:56 → 421

3a0801s03tim44; mitochondrial import inner membrane, translocase subunit