POB3 FACT complex subunit POB3 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: POB3
Official Full Name: FACT complex subunit POB3
Primary source: SGD:S000004534
Locus tag: YML069W
See related: AllianceGenome:SGD:S000004534; FungiDB:YML069W; VEuPathDB:YML069W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Contributes to histone binding activity and nucleosome binding activity. Involved in DNA-templated DNA replication; chromatin organization; and positive regulation of RNA polymerase II transcription preinitiation complex assembly. Located in chromatin. Part of FACT complex and replication fork protection complex. Used to study cancer. Orthologous to human SSRP1 (structure specific recognition protein 1). [provided by Alliance of Genome Resources, Nov 2024]
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Genomic context

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See POB3 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (135500..137158)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.
Title: Electron microscopy analysis of ATP-independent nucleosome unfolding by FACT.
Intrinsic DNA-bending activity therefore favors nucleosome assembly by FACT over nucleosome reorganization, but excessive activity impairs FACT release, suggesting a quality control checkpoint during nucleosome assembly
Title: Functional roles of the DNA-binding HMGB domain in the histone chaperone FACT in nucleosome reorganization.
Results provide evidence that both FACT and Spt6 are important for transcription globally and may participate during different stages of transcription.
Title: Acetylation-Dependent Recruitment of the FACT Complex and Its Role in Regulating Pol II Occupancy Genome-Wide in Saccharomyces cerevisiae.
FACT remodels the tetranucleosomal unit of chromatin fibers for gene transcription.
Title: FACT Remodels the Tetranucleosomal Unit of Chromatin Fibers for Gene Transcription.
FACT binding results in dramatic ATP-independent, symmetrical and reversible DNA uncoiling that affects at least 70% of the DNA within a nucleosome. Nucleosome unfolding by yFACT (Spt16-Pob3 with Nhp6) is extensive and reversible.
Title: Large-scale ATP-independent nucleosome unfolding by a histone chaperone.
Histone chaperones FACT and Spt6 are important for appropriate H2AZ histone localization and for the functioning of RNA polymerase II.
Title: Histone chaperones FACT and Spt6 prevent histone variants from turning into histone deviants.
Spt6 and FACT have both overlapping and distinct functional interactions with histones and nucleosomes and that binding to each of the components of a nucleosome is not sufficient to produce nucleosome reorganization
Title: The Abundant Histone Chaperones Spt6 and FACT Collaborate to Assemble, Inspect, and Maintain Chromatin Structure in Saccharomyces cerevisiae.
FACT, a heterodimer of Spt16 and Pob3, disrupts nucleosome structure by binding H2A-H2B with conserved peptide motifs.
Title: FACT Disrupts Nucleosome Structure by Binding H2A-H2B with Conserved Peptide Motifs.
Data show that the histone chaperones POB3 protein FACT and chromatin-remodeling histone chaperone Spt6 prevent widespread accumulation of histone H2A.Z.
Title: The Histone Chaperones FACT and Spt6 Restrict H2A.Z from Intragenic Locations.
Pob3-Q308K caused inefficient dissociation of FACT from nucleosomes.
Title: The FACT histone chaperone guides histone H4 into its nucleosomal conformation in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
contributes_to chromatin binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to histone binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to nucleosome binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA-templated DNA replication	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA-templated DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA-templated DNA replication	IPI Inferred from Physical Interaction more info	PubMed
involved_in chromatin organization	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of RNA polymerase II transcription preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
part_of FACT complex	IBA Inferred from Biological aspect of Ancestor more info
part_of FACT complex	IDA Inferred from Direct Assay more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome	IEA Inferred from Electronic Annotation more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: FACT complex subunit POB3

NP_013642.1

Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

135500..137158

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182428.1 → NP_013642.1 TPA: FACT complex subunit POB3 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013642.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZA4, Q04636

UniProtKB/TrEMBL

A6ZLZ6, B3LLI6, B5VP62, C7GW05, C8ZED6, G2WJY6, N1NYY8

Conserved Domains (1) summary

COG5165
Location:1 → 546

POB3; Nucleosome-binding factor SPN, POB3 subunit [Transcription / DNA replication, recombination, and repair / Chromatin structure and dynamics]