PPZ1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PPZ1
Primary source: FungiDB:YML016C
Locus tag: YML016C
See related: SGD:S000004478; AllianceGenome:SGD:S000004478; VEuPathDB:YML016C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables protein serine/threonine phosphatase activity. Involved in cellular protein localization; cellular sodium ion homeostasis; and protein dephosphorylation. Located in nucleus. Is extrinsic component of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See PPZ1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (239458..241536, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Functional mapping of the N-terminal region of the yeast moonlighting protein Sis2/Hal3 reveals crucial residues for Ppz1 regulation.
Title: Functional mapping of the N-terminal region of the yeast moonlighting protein Sis2/Hal3 reveals crucial residues for Ppz1 regulation.
The toxic effects of yeast Ppz1 phosphatase are counteracted by subcellular relocalization mediated by its regulatory subunit Hal3.
Title: The toxic effects of yeast Ppz1 phosphatase are counteracted by subcellular relocalization mediated by its regulatory subunit Hal3.
Overexpression of budding yeast protein phosphatase Ppz1 impairs translation.
Title: Overexpression of budding yeast protein phosphatase Ppz1 impairs translation.
Site-directed mutagenesis results indicate mutation of ScHal3 L403 has no effect on trimerization and phosphopantothenoyl-cysteine decarboxylase function. ScHal3 L405E fails to form homotrimers, but retains the capacity to bind Cab3. Remarkably, the L405E mutation decreases Hal3's ability to interact with and to inhibit Ppz1, confirming the importance of the oligomer/monomer equilibrium in Hal3's Ppz1 regulating function.
Title: Mutations at the hydrophobic core affect Hal3 trimer stability, reducing its Ppz1 inhibitory capacity but not its PPCDC moonlighting function.
Caffeine sensitivity of the Deltappz1 disruptant is suppressed by disruption of either SAT4 or HAL5. Ppz1 controls Gln3 localization by regulating its phosphorylation state in combination with Sat4 and Hal5.
Title: Saccharomyces cerevisiae protein phosphatase Ppz1 and protein kinases Sat4 and Hal5 are involved in the control of subcellular localization of Gln3 by likely regulating its phosphorylation state.
The aim of this work was to study mechanisms which relate the activity of Ppz1p/Hal3p complex to nonsense suppression efficiency.
Title: [The protein complex Ppz1p/Hal3p and nonsense suppression efficiency in the yeast Saccharomyces cerevisiae].
Overexpression of gene PPZ1 in the yeast Saccharomyces cerevisiae affects the efficiency of nonsense suppression
Title: [Overexpression of gene PPZ1 in the yeast Saccharomyces cerevisiae affects the efficiency of nonsense suppression].
Results indicate that, in addition to their role in regulating Trk1 and 2 potassium transporters, Ppz phosphatases 1 and 2 positively affect the residual low affinity potassium transport mechanisms in yeast.
Title: The Ppz protein phosphatases regulate Trk-independent potassium influx in yeast.
the Ppz1-Hal3 interaction is a sensor of intracellular pH that modulates H+ and K+ homeostasis through the regulation of Trk1p activity
Title: pH-Responsive, posttranslational regulation of the Trk1 potassium transporter by the type 1-related Ppz1 phosphatase.
Results demonstrate the participation of the Hal3-Ppz1 protein complex in regulation of read-through efficiency and manifestation of non-Mendelian anti-suppressor determinant [ISP(+)].
Title: The HAL3-PPZ1 dependent regulation of nonsense suppression efficiency in yeast and its influence on manifestation of the yeast prion-like determinant [ISP(+)].

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables myosin phosphatase activity	IEA Inferred from Electronic Annotation more info
enables phosphoprotein phosphatase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine phosphatase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine phosphatase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein serine/threonine phosphatase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in intracellular sodium ion homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: salt homeostasis regulator

NP_013696.1

EC 3.1.3.16

Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

239458..241536 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182374.1 → NP_013696.1 TPA: salt homeostasis regulator [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013696.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZF8, P26570, Q00979

UniProtKB/TrEMBL

A6ZM46, B3LLN6, B5VPA9, C7GLD4, C8ZEI8, G2WK36, N1NYJ4

Conserved Domains (1) summary

cd07414
Location:362 → 653

MPP_PP1_PPKL; PP1, PPKL (PP1 and kelch-like) enzymes, and related proteins, metallophosphatase domain