YPT7 Rab family GTPase YPT7 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Gene symbol: YPT7
Gene description: Rab family GTPase YPT7
Primary source: FungiDB:YML001W
Locus tag: YML001W
See related: SGD:S000004460; AllianceGenome:SGD:S000004460; VEuPathDB:YML001W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: AST4; VAM4
Summary: Enables GTPase activity. Involved in several processes, including cytoplasm to vacuole transport by the Cvt pathway; piecemeal microautophagy of the nucleus; and vacuole inheritance. Located in fungal-type vacuole membrane and vacuole-mitochondrion membrane contact site. Human ortholog(s) of this gene implicated in Charcot-Marie-Tooth disease type 2B. Orthologous to human RAB7A (RAB7A, member RAS oncogene family). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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See YPT7 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (267174..267800)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes.
Title: The GTPase activating protein Gyp7 regulates Rab7/Ypt7 activity on late endosomes.
In this study, we demonstrate that activated Ypt7p localizes to the vacuolar membrane in cells lacking all yeast Rab5 genes. We also show that the vps21Delta ypt7Delta double mutant exhibits a much severer vacuolar phenotype in comparison with the vps21Delta or ypt7Delta single mutant.
Title: Rab5-independent activation and function of yeast Rab7-like protein, Ypt7p, in the AP-3 pathway.
Homotypic fusion and protein-sorting/class C vacuole protein-sorting (HOPS/class C Vps) complex can tether low-curvature membranes, that is, liposomes with a diameter of 100 nm, only when the yeast vacuolar Ypt7p is present in both tethered membranes.
Title: The HOPS/class C Vps complex tethers membranes by binding to one Rab GTPase in each apposed membrane.
Guanine nucleotide exchange factor activity of the Mon1-Ccz1 complex towards Ypt7 requires the interface formed by their longin domains.
Title: The Mon1-Ccz1 GEF activates the Rab7 GTPase Ypt7 via a longin-fold-Rab interface and association with PI3P-positive membranes.
phosphorylation of vacuole protein sorting complex (HOPS) by recombinant Yck3p confers strict requirement for GTP-bound Ypt7p for binding phosphorylated HOPS, for optimal membrane tethering and proteoliposome fusion; guanine nucleotide specificity of the vacuolar fusion Rab Ypt7p is conferred through downstream posttranslational modification of its effector complex
Title: Phosphorylation of the effector complex HOPS by the vacuolar kinase Yck3p confers Rab nucleotide specificity for vacuole docking and fusion.
msb3 vacuoles fuse more efficiently than wild-type vacuoles in vitro, suggesting that GAP can also act on Ypt7
Title: The Msb3/Gyp3 GAP controls the activity of the Rab GTPases Vps21 and Ypt7 at endosomes and vacuoles.
yeast retromer trimer antagonizes Ypt7-regulated organelle tethering and fusion of endosomes/vacuoles via recognition of Ypt7
Title: Rab GTPase regulation of retromer-mediated cargo export during endosome maturation.
Ypt7p plays a role beyond homotypic fusion and protein sorting-Class C Vps complex recruitment in membrane fusion
Title: The yeast vacuolar Rab GTPase Ypt7p has an activity beyond membrane recruitment of the homotypic fusion and protein sorting-Class C Vps complex.
Ypt7 functions at the late endosome to coordinate retromer-mediated recycling with the fusion of late endosomes with vacuoles.
Title: The Rab GTPase Ypt7 is linked to retromer-mediated receptor recycling and fusion at the yeast late endosome.
We also show that expression of a mutated variant of the YPT7 gene suppresses the sporulation and autophagy defects of ccz1Delta cells to a quantitatively similar level.
Title: Mutants of the Saccharomyces cerevisiae VPS genes CCZ1 and YPT7 are blocked in different stages of sporulation.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IDA Inferred from Direct Assay more info	PubMed
enables GTPase activity	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in cytoplasm to vacuole targeting by the Cvt pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein localization to vacuole	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info
involved_in regulation of protein-containing complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of vacuole fusion, non-autophagic	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of vacuole fusion, non-autophagic	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde transport, endosome to Golgi	IPI Inferred from Physical Interaction more info	PubMed
involved_in vacuole inheritance	IDA Inferred from Direct Assay more info	PubMed
involved_in vesicle-mediated transport	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in fungal-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in intracellular organelle	IEA Inferred from Electronic Annotation more info
is_active_in late endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in late endosome	IEA Inferred from Electronic Annotation more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in mitochondrial outer membrane	HDA more info	PubMed
located_in mitochondrion	HDA more info	PubMed
located_in multivesicular body	IDA Inferred from Direct Assay more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
is_active_in vacuole	IBA Inferred from Biological aspect of Ancestor more info
located_in vacuole	IEA Inferred from Electronic Annotation more info
located_in vacuole-mitochondrion membrane contact site	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Rab family GTPase YPT7

NP_013713.1

Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7