SUB1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SUB1
Primary source: SGD:S000004642
Locus tag: YMR039C
See related: AllianceGenome:SGD:S000004642; FungiDB:YMR039C; VEuPathDB:YMR039C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TSP1
Summary: Enables G-quadruplex DNA binding activity and chromatin binding activity. Involved in several processes, including negative regulation of ascospore formation; nucleic acid metabolic process; and regulation of transcription, DNA-templated. Located in nucleus. Orthologous to human SUB1 (SUB1 regulator of transcription). [provided by Alliance of Genome Resources, Apr 2022]
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Genomic context

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Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (348644..349522, complement)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.
Title: Pif1 Helicase Mediates Remodeling of Protein-Nucleic Acid Complexes by Promoting Dissociation of Sub1 from G-Quadruplex DNA and Cdc13 from G-Rich Single-Stranded DNA.
The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain.
Title: The Role of S. cerevisiae Sub1/PC4 in Transcription Elongation Depends on the C-Terminal Region and Is Independent of the ssDNA Binding Domain.
Evolutionarily conserved role for Sub1 and PC4 in the maintenance of yeast and human genome stability has been summarized. (Review)
Title: Sub1/PC4, a multifaceted factor: from transcription to genome stability.
Yeast Sub1 and human PC4 are G-quadruplex binding proteins that suppress genome instability at co-transcriptionally formed G4 DNA.
Title: Yeast Sub1 and human PC4 are G-quadruplex binding proteins that suppress genome instability at co-transcriptionally formed G4 DNA.
demonstrate that physical and genetic interactions exist between Hot1 and several proteins involved in transcriptional and posttranscriptional processes: for example, transcription co-activator Sub1 and elongation complex Spt4/5
Title: Hot1 factor recruits co-activator Sub1 and elongation complex Spt4/5 to osmostress genes.
demonstrate that purified Sub1 protein can protect DNA from oxidative damage in vitro, using the metal ion catalyzed oxidation assay
Title: The Sub1 nuclear protein protects DNA from oxidative damage.
This hypothesis was reinforced by the finding that inactivation of Sub1 transcription initiation factor, which is found predominantly in the regions of transcription initiation, leads to further increase in mutagenesis in the beginning of the genes.
Title: Disruption of Transcriptional Coactivator Sub1 Leads to Genome-Wide Re-distribution of Clustered Mutations Induced by APOBEC in Active Yeast Genes.
results suggest that SUB1 acts as a negative regulator of sporulation.
Title: SUB1 Plays a Negative Role during Starvation Induced Sporulation Program in Saccharomyces cerevisiae.
Sub1, and its human homolog PC4, bind preferentially to G-quadruplexes
Title: Yeast transcription co-activator Sub1 and its human homolog PC4 preferentially bind to G-quadruplex DNA.
Sub1 and Maf1 also exhibit an opposite role on yeast chronological life span
Title: Sub1 and Maf1, two effectors of RNA polymerase III, are involved in the yeast quiescence cycle.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables G-quadruplex DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables chromatin binding	IDA Inferred from Direct Assay more info	PubMed
enables transcription coactivator activity	IBA Inferred from Biological aspect of Ancestor more info
enables transcription coactivator activity	IEA Inferred from Electronic Annotation more info
enables transcription coactivator activity	ISS Inferred from Sequence or Structural Similarity more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA polymerase III preinitiation complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to pheromone	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to stress	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular response to stress	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular response to stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in double-strand break repair via nonhomologous end joining	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of ascospore formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription by RNA polymerase III	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of transcription by RNA polymerase III	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription initiation by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in termination of RNA polymerase II transcription	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of transcription regulator complex	IBA Inferred from Biological aspect of Ancestor more info

General protein information

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Preferred Names: chromatin-binding transcription coactivator SUB1

NP_013753.1

Transcriptional regulator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; negatively regulates sporulation; protein abundance increases in response to DNA replication stress; functionally complemented by human SUB1 (PC4)

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

348644..349522 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182536.1 → NP_013753.1 TPA: chromatin-binding transcription coactivator SUB1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013753.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VZL4, P54000

UniProtKB/TrEMBL

A6ZMA1, B3LLT8, C7GL80, C8ZEP6, G2WK92, N1NYN1

Conserved Domains (1) summary

pfam02229
Location:45 → 94

PC4; Transcriptional Coactivator p15 (PC4)