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SGS1 ATP-dependent DNA helicase SGS1 [ Saccharomyces cerevisiae S288C ]

Gene ID: 855228, updated on 28-Oct-2024

Summary

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Official Symbol
SGS1
Official Full Name
ATP-dependent DNA helicase SGS1
Primary source
SGD:S000004802
Locus tag
YMR190C
See related
AllianceGenome:SGD:S000004802; FungiDB:YMR190C; VEuPathDB:YMR190C
Gene type
protein coding
RefSeq status
REVIEWED
Organism
Saccharomyces cerevisiae S288C (strain: S288C)
Lineage
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary
Enables 3'-5' DNA helicase activity. Involved in several processes, including DNA metabolic process; negative regulation of cell cycle process; and nuclear chromosome segregation. Located in nucleus. Part of RecQ family helicase-topoisomerase III complex. Is active in nucleolus. Used to study Bloom syndrome and Werner syndrome. Human ortholog(s) of this gene implicated in Bloom syndrome. Orthologous to human BLM (BLM RecQ like helicase). [provided by Alliance of Genome Resources, Oct 2024]
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Genomic context

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See SGS1 in Genome Data Viewer
Location:
chromosome: XIII
Exon count:
1
Sequence:
Chromosome: XIII; NC_001145.3 (640915..645258, complement)

Chromosome XIII - NC_001145.3Genomic Context describing neighboring genes Neighboring gene mitochondrial 37S ribosomal protein MRPS17 Neighboring gene glycine decarboxylase subunit P Neighboring gene Spg5p Neighboring gene Gyl1p

Genomic regions, transcripts, and products

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Genomic Sequence:
NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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Related articles in PubMed

  1. Redundancy of DNA helicases in p53-mediated apoptosis. Spillare EA, et al. Oncogene, 2006 Mar 30. PMID 16288211, Free PMC Article
  2. Mitotic inter-homologue junctions accumulate at damaged DNA replication forks in recQ mutants. Carotenuto W, et al. DNA Repair (Amst), 2010 Jun 4. PMID 20346738
  3. Elevation of sister chromatid exchange in Saccharomyces cerevisiae sgs1 disruptants and the relevance of the disruptants as a system to evaluate mutations in Bloom's syndrome gene. Onoda F, et al. Mutat Res, 2000 Apr 28. PMID 10812332
  4. The full-length Saccharomyces cerevisiae Sgs1 protein is a vigorous DNA helicase that preferentially unwinds holliday junctions. Cejka P, et al. J Biol Chem, 2010 Mar 12. PMID 20086270, Free PMC Article
  5. Genomic distribution and functional analyses of potential G-quadruplex-forming sequences in Saccharomyces cerevisiae. Hershman SG, et al. Nucleic Acids Res, 2008 Jan. PMID 17999996, Free PMC Article

See all (493) citations in PubMed

GeneRIFs: Gene References Into Functions

What's a GeneRIF?
  1. Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.
    Title: Checkpoint phosphorylation sites on budding yeast Rif1 protect nascent DNA from degradation by Sgs1-Dna2.
  2. Multifaceted regulation of the sumoylation of the Sgs1 DNA helicase.
    Title: Multifaceted regulation of the sumoylation of the Sgs1 DNA helicase.
  3. C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes of Saccharomyces cerevisiae Bloom's Helicase Homolog Sgs1 SUMOylation-Associated Mutants.
    Title: C-Terminal HA Tags Compromise Function and Exacerbate Phenotypes of Saccharomyces cerevisiae Bloom's Helicase Homolog Sgs1 SUMOylation-Associated Mutants.
  4. Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.
    Title: Comprehensive Synthetic Genetic Array Analysis of Alleles That Interact with Mutation of the Saccharomyces cerevisiae RecQ Helicases Hrq1 and Sgs1.
  5. Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis.
    Title: Phosphorylation of the RecQ Helicase Sgs1/BLM Controls Its DNA Unwinding Activity during Meiosis and Mitosis.
  6. Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases.
    Title: Maintenance of Yeast Genome Integrity by RecQ Family DNA Helicases.
  7. Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway
    Title: Deletion of ULS1 confers damage tolerance in sgs1 mutants through a Top3-dependent D-loop mediated fork restart pathway.
  8. Results suggest that the differential regulation of Sgs1 activity by its protein partners is important to support diverse cellular functions of Sgs1 during the maintenance of genome stability.
    Title: Competing interaction partners modulate the activity of Sgs1 helicase during DNA end resection.
  9. we find that Sgs1 utilizes a novel motor mechanism for disrupting ssDNA intermediates bound by the recombinase protein Rad51. The ability of Sgs1 to disrupt Rad51-ssDNA filaments may explain some of the defects engendered by RECQ helicase deficiencies in human cells
    Title: The RecQ helicase Sgs1 drives ATP-dependent disruption of Rad51 filaments.
  10. DNA nick created by MRX-Sae2 at Ku-blocked DNA double-strand breaks ends can serve as the entry site for Exo1 or Sgs1-Dna2.
    Title: A DNA nick at Ku-blocked double-strand break ends serves as an entry site for exonuclease 1 (Exo1) or Sgs1-Dna2 in long-range DNA end resection.
Submit: New GeneRIF Correction See all GeneRIFs (66)

Interactions

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Products Interactant Other Gene Complex Source Pubs Description

General gene information

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Homology

Gene Ontology Provided by SGD

Function Evidence Code Pubs
enables 3'-5' DNA helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables 3'-5' DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables 3'-5' DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables ATP binding IEA
Inferred from Electronic Annotation
more info
  
enables ATP hydrolysis activity IEA
Inferred from Electronic Annotation
more info
  
enables DNA binding IEA
Inferred from Electronic Annotation
more info
  
enables DNA helicase activity IDA
Inferred from Direct Assay
more info
 PubMed 
enables DNA helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables four-way junction helicase activity IBA
Inferred from Biological aspect of Ancestor
more info
  
enables helicase activity IEA
Inferred from Electronic Annotation
more info
  
enables hydrolase activity IEA
Inferred from Electronic Annotation
more info
  
enables isomerase activity IEA
Inferred from Electronic Annotation
more info
  
enables nucleic acid binding IEA
Inferred from Electronic Annotation
more info
  
enables zinc ion binding RCA
inferred from Reviewed Computational Analysis
more info
 PubMed 
Process Evidence Code Pubs
involved_in DNA double-strand break processing IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in DNA double-strand break processing IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in DNA duplex unwinding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA recombination IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA repair IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA replication IEA
Inferred from Electronic Annotation
more info
  
involved_in DNA topological change IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in DNA unwinding involved in DNA replication IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in DNA unwinding involved in DNA replication IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in G-quadruplex DNA unwinding IDA
Inferred from Direct Assay
more info
 PubMed 
involved_in chromosome organization IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in double-strand break repair via homologous recombination IBA
Inferred from Biological aspect of Ancestor
more info
  
involved_in double-strand break repair via homologous recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in double-strand break repair via homologous recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in gene conversion at mating-type locus IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in meiotic DNA double-strand break processing IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in meiotic chromosome segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic G2/M transition checkpoint IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic intra-S DNA damage checkpoint signaling IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in mitotic intra-S DNA damage checkpoint signaling IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in mitotic sister chromatid segregation IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in negative regulation of meiotic joint molecule formation IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in regulation of reciprocal meiotic recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance via recombination IGI
Inferred from Genetic Interaction
more info
 PubMed 
involved_in telomere maintenance via recombination IMP
Inferred from Mutant Phenotype
more info
 PubMed 
involved_in telomeric 3' overhang formation IGI
Inferred from Genetic Interaction
more info
 PubMed 
Component Evidence Code Pubs
part_of RecQ family helicase-topoisomerase III complex IDA
Inferred from Direct Assay
more info
 PubMed 
part_of RecQ family helicase-topoisomerase III complex IPI
Inferred from Physical Interaction
more info
 PubMed 
is_active_in chromosome IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in cytoplasm IBA
Inferred from Biological aspect of Ancestor
more info
  
is_active_in nucleolus IDA
Inferred from Direct Assay
more info
 PubMed 
located_in nucleolus IEA
Inferred from Electronic Annotation
more info
  
located_in nucleus HDA PubMed 
is_active_in nucleus IBA
Inferred from Biological aspect of Ancestor
more info
  
located_in nucleus IEA
Inferred from Electronic Annotation
more info
  
part_of protein-containing complex IEA
Inferred from Electronic Annotation
more info
  

General protein information

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Preferred Names
ATP-dependent DNA helicase SGS1
NP_013915.1
  • RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

  1. NC_001145.3 Reference assembly

    Range
    640915..645258 complement
    Download
    GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

  1. NM_001182696.1 → NP_013915.1  TPA: ATP-dependent DNA helicase SGS1 [Saccharomyces cerevisiae S288C]

    See identical proteins and their annotated locations for NP_013915.1

    Status: REVIEWED

    UniProtKB/Swiss-Prot
    D6W014, P35187
    UniProtKB/TrEMBL
    C7GRG1
    Conserved Domains (1) summary
    COG0514
    Location:670 → 1350
    RecQ; Superfamily II DNA helicase RecQ [Replication, recombination and repair]
Nucleotide Protein
Heading Accession and Version
Protein Accession Links
GenPept Link UniProtKB Link
P35187.1

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